Microspheres based on inulin for the controlled release of serine protease inhibitors: preparation, characterization and in vitro release

Poulain, Nathalie; Dez, Isabelle; Perrio, Cécile; Lasne, Marie‐Claire; Prud'Homme, Marie-Pascale; Nakache, E.

doi:10.1016/s0168-3659(03)00251-7

Cited by 53 publications

(41 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The amount of entrapped Hb was determined indirectly, i.e., by measuring the difference amount of Hb between the initial amount of Hb (Hb total ) and amount of free Hb in the supernatant (Hb free ). So the EE% was expressed as following equation [11]: EE% ¼ ðHb total À Hb free Þ=Hb total Â 100%.…”

Section: Determination Of Ee% and Pce%mentioning

confidence: 99%

Preparation of hemoglobin-loaded nano-sized particles with porous structure as oxygen carriers

et al. 2007

View full text Add to dashboard Cite

Section: Determination Of Ee% and Pce%mentioning

confidence: 99%

Preparation of hemoglobin-loaded nano-sized particles with porous structure as oxygen carriers

et al. 2007

View full text Add to dashboard Cite

“…In particular, the choice of inulin as a starting polymer was based on the peculiar properties of this polysaccharide; it is specifically degraded into the colon, non-toxic (including its degradation products), shows a prebiotic effect on the intestinal bacterial population, is widely used in the food industry, is FDA approved and has been proposed as a starting material for the production of drug delivery systems. [4,7,[29][30][31][32][33][34][35][36][37][38][39] Taking into consideration the interesting physicochemical properties of INUDVSA-TT hydrogels, such as cell compatibility, pH-sensitivity and biodegradability, they were soaked with 2-metoxyestradiol (2-ME), chosen as an anti-cancer model drug. 2-ME is an endogenous metabolite of estrogen, which is synthesized in vivo by hydroxylation at the 2-position of 17b-estradiol by cytochrome P450 enzymes, and subsequent catechol-O-methyltransferase (COMT) mediated O-methylation.…”

Section: Introductionmentioning

confidence: 99%

Hydrogels for Potential Colon Drug Release by Thiol‐ene Conjugate Addition of a New Inulin Derivative

Pitarresi

Tripodo

Calabrese

et al. 2008

Macromolecular Bioscience

View full text Add to dashboard Cite

Inulin was chosen as a starting polymer for biocompatible, pH-sensitive and biodegradable hydrogels. Three INUDVSA-TT hydrogels were obtained by crosslinking inulin derivatives with trimethylolpropane tris(3-mercaptopropionate) under varying conditions. The resulting hydrogels were cell compatible, as demonstrated by MTS and trypan blue exclusion assays acting on Caco-2 cells, and were biodegraded by inulinase and esterase, thus suggesting their use as colonic drug delivery systems. 2-Methoxyestradiol, an anti-cancer drug, was soaked in INUDVSA-TT hydrogels and its in vitro release and apoptotic effect on Caco-2 cells were evaluated.

show abstract

“…A similar procedure had previously been adopted by others. 29 The maximum solubility measured for khusimyl dansylate (36.3 mg KD/ml anhydrous alcohol) was much greater than that used in the release assays (2.08 × 10 −3 mg KD/ml ethanol). Also, vetiver oil presents high solubility, around 80%, in ethanol.…”

Section: Resultsmentioning

confidence: 87%

Encapsulation and release of a fluorescent probe, khusimyl dansylate, obtained from vetiver oil by complex coacervation

Prata

Menut

Leydet

et al. 2007

Flavour & Fragrance J

View full text Add to dashboard Cite

ABSTRACT:The essential oil of vetiver [Vetiveria zizanoides (L.) Nash ex. Small] is widely used in the perfume industry, owing to its pleasant, long-lasting, woody aroma. If this substance can be encapsulated in microparticles so that its release can be controlled, the effective duration of its properties should be extended for a much longer period of time. The present study was thus designed to investigate the encapsulation of this vetiver essential oil in microparticles. Since the detection of the effective release of such a complex mixture from these microparticles into the receiving medium can be problematic, an identifiable probe can be released with it to facilitate evaluation of the progression of the release process. Zizanoic acid is one of the compounds found in vetiver oil which depreciates its sensorial quality. This acid was thus extracted and reduced to the corresponding alcohol, khusimol, which was combined with dansyl chloride to form a fluorescent ester, khusimyl dansylate (KD). The vetiver oil and the fluorescent probe were then encapsulated (100:1) in microparticles produced by the complex coacervation of gum Arabic and gelatin. The microparticles showed spherical shape, multinuclear distribution of the core material and high encapsulation efficiency (95%). Two versions of these microparticles, moist and freeze-dried ones, were tested for the release of the KD into an ethanol medium. The moist particles released the whole KD after 5 h, although only 80% of the fluorescent probe was released with the freeze-dried microparticles at that time, probably due to the constriction caused by freeze-drying. The release of the components of vetiver oil, under the same experimental conditions, was followed, in parallel, by gas chromatography and the results obtained were compared and discussed.

show abstract

Microspheres based on inulin for the controlled release of serine protease inhibitors: preparation, characterization and in vitro release

Cited by 53 publications

References 31 publications

Preparation of hemoglobin-loaded nano-sized particles with porous structure as oxygen carriers

Preparation of hemoglobin-loaded nano-sized particles with porous structure as oxygen carriers

Hydrogels for Potential Colon Drug Release by Thiol‐ene Conjugate Addition of a New Inulin Derivative

Encapsulation and release of a fluorescent probe, khusimyl dansylate, obtained from vetiver oil by complex coacervation

Contact Info

Product

Resources

About