Microsporogenesis of Rps8/rps8 heterozygous soybean lines

Ortega, María A.; Dorrance, Anne E.

doi:10.1007/s10681-011-0422-1

Cited by 8 publications

(4 citation statements)

References 38 publications

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“…The mapping was similar for the LOD range and peak SNP across generations and in some populations across isolates. This phenomenon has been reported previously in studies of crosses with PIs and Williams ( Gordon et al., 2007a ; Ortega and Dorrance, 2011 ). This could be in part due to meiotic incompatibility ( Ortega and Dorrance, 2011 ) or for other chromosomal challenges in the complex R gene loci.…”

Section: Discussionsupporting

confidence: 84%

Comparison of Rps loci toward isolates, singly and combined inocula, of Phytophthora sojae in soybean PI 407985, PI 408029, PI 408097, and PI424477

Clevinger,

Biyashev,

Schmidt

et al. 2024

Front. Plant Sci.

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For soybean, novel single dominant Resistance to Phytophthora sojae (Rps) genes are sought to manage Phytophthora root and stem rot. In this study, resistance to P. sojae was mapped individually in four recombinant inbred line (RIL) populations derived from crosses of the susceptible cultivar Williams with PI 407985, PI 408029, PI 408097, and PI424477 previously identified as putative novel sources of disease resistance. Each population was screened for resistance with five to seven isolates of P. sojae separately over multiple F7–F10 generations. Additionally, three of the populations were screened with inoculum from the combination of three P. sojae isolates (PPR), which comprised virulence to 14 Rps genes. Over 2,300 single-nucleotide polymorphism markers were used to construct genetic maps in each population to identify chromosomal regions associated with resistance to P. sojae. Resistance segregated as one or two genes to the individual isolates and one gene toward PPR in each population and mapped to chromosomes 3, 13, or 18 in one or more of the four RIL populations. Resistance to five isolates mapped to the same chromosome 3 region are as follows: OH7 (PI 424477 and PI408029), OH12168, OH7/8, PPR (PI 407985), and 1.S.1.1 (PI408029). The resistance regions on chromosome 13 also overlapped for OH1, OH25, OH-MIA (PI424477), PPR (PI 424477, PI 407985, and PI 408097), PPR and OH0217 (PI 408097), and OH4 (PI 408029), but were distinct for each population suggesting multiple genes confer resistance. Two regions were identified on chromosome 18 but all appear to map to known loci; notably, resistance to the combined inoculum (PPR) did not map at this locus. However, there are putative new alleles in three of four populations, three on chromosome 3 and two on chromosome 13 based on mapping location but also known virulence in the isolate used. This characterization of all the Rps genes segregating in these populations to these isolates will be informative for breeding, but the combined inoculum was able to map a novel loci. Furthermore, within each of these P. sojae isolates, there was virulence to more than the described Rps genes, and the effectiveness of the novel genes requires testing in larger populations.

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Section: Discussionsupporting

confidence: 84%

Comparison of Rps loci toward isolates, singly and combined inocula, of Phytophthora sojae in soybean PI 407985, PI 408029, PI 408097, and PI424477

Clevinger,

Biyashev,

Schmidt

et al. 2024

Front. Plant Sci.

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“…In the case of the zebra finch, the germline-restricted chromosome fragments remain detectable in the cell cytoplasm as a densely packed chromatin body until the end of meiosis ( Goday and Pigozzi, 2010 ; Schoenmakers et al, 2010 ). Micronucleation is also observed in plant microsporogenesis, for example, of Avena sativa and Glycine max , when part of chromosomes remains beyond the nucleus during division, further separating as affected small microsporocytes, and eventually giving small sterile pollen ( Baptista-Giacomelli et al, 2000 ; Ortega and Dorrance, 2011 ). Despite careful analysis, we have not detected any small microsporocytes during microsporogenesis of tobacco plants ( Mursalimov and Deineko, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Cytomixis doesn’t induce obvious changes in chromatin modifications and programmed cell death in tobacco male meiocytes

et al. 2015

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Cytomixis is a poorly studied process of nuclear migration between plant cells. It is so far unknown what drives cytomixis and what is the functional state of the chromatin migrating between cells. Using immunostaining, we have analyzed the distribution of posttranslational histone modifications (methylation, acetylation, and phosphorylation) that reflect the functional state of chromatin in the tobacco microsporocytes involved in cytomixis. We demonstrate that the chromatin in the cytomictic cells does not differ from the chromatin in intact microsporocytes according to all 14 analyzed histone modification types. We have also for the first time demonstrated that the migrating chromatin contains normal structures of the synaptonemal complex (SC) and lacks any signs of apoptosis. As has been shown, the chromatin migrating between cells in cytomixis is neither selectively heterochromatized nor degraded both before its migration to another cell and after it enters a recipient cell as micronuclei. We also showed that cytomictic chromatin contains marks typical for transcriptionally active chromatin as well as heterochromatin. Moreover, marks typical for chromosome condensation, SC formation and key proteins required for the formation of bivalents were also detected at migrated chromatin.

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“…Soybean resistance to P. sojae is mainly controlled by two mechanisms, involving complete or partial resistance genes [2,3]. The former type of resistance is related to a single dominant resistance gene [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19], with P. sojae interacting with Rps genes in a gene-for-gene system preventing disease development in plants [20], while the latter involves multiple genes and limits damage to the plant [3,21].…”

Section: Introductionmentioning

confidence: 99%

Fine mapping of a Phytophthora -resistance locus RpsGZ in soybean using genotyping-by-sequencing

Jiang

Cheng

Cai

et al. 2020

Preprint

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Background: Phytophthora root rot (PRR) caused by Phytophthora sojae ( P. sojae ), is one of the most serious limitation to soybean-production in the world. Identification of resistant gene(s) and incorporating them into elite varieties are an effective way for breeding to prevent soybean from being harmed by this disease. A valuable mapping population of 228 F 8:11 recombinant inbred lines (RILs) derived from a cross of resistant cultivar Guizao1 and susceptible cultivar BRSMG68 and a high-density genetic linkage map with an average distance of 0.81 centimorgan (cM) between adjacent bin markers in this population were used to map and explore the candidate gene(s).Results: In this study, the PRR resistance in Guizao1 was controlled by a single Mendelian locus, and was fine mapped to a 367.371-kb genomic region on chromosome 3 that harbours 19 genes, including 7 disease resistance (R)-like genes in the reference Willliams 82 genome. Quantitative real-time PCR assays of possible candidate genes revealed that Glyma.03g05300 was likely involved in PRR resistance.Conclusions: These findings of fine mapping of a novel Rps locus will serve as a basis for cloning, transferring of resistant genes and breeding of P. sojae resistant soybean cultivars through marker-assisted selection.

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Microsporogenesis of Rps8/rps8 heterozygous soybean lines

Cited by 8 publications

References 38 publications

Comparison of Rps loci toward isolates, singly and combined inocula, of Phytophthora sojae in soybean PI 407985, PI 408029, PI 408097, and PI424477

Comparison of Rps loci toward isolates, singly and combined inocula, of Phytophthora sojae in soybean PI 407985, PI 408029, PI 408097, and PI424477

Cytomixis doesn’t induce obvious changes in chromatin modifications and programmed cell death in tobacco male meiocytes

Fine mapping of a Phytophthora -resistance locus RpsGZ in soybean using genotyping-by-sequencing

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