Microstructural analysis of demineralized primary enamel after in vitro toothbrushing

Neves, Aline de Almeida; Castro, Rodolfo; Coutinho, Eduardo; Primo, Laura Guimarães

doi:10.1590/s1517-74912002000200008

Cited by 14 publications

(20 citation statements)

References 14 publications

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“…This softened layer was vulnerable to mechanical forces, and was removed by the brushing procedure, resulting in exposure of a harder enamel surface. The scanning electron microscopy images also corroborate this hypothesis 26 . Due to this fact, the wear increased when eroded enamel was subjected to abrasion, but the %SMHC was lower when compared to that of erosion alone.…”

Section: Discussionsupporting

confidence: 67%

Influence of toothbrushing on enamel softening and abrasive wear of eroded bovine enamel: an in situ study

Ríos

Honório²,

Magalhães³

et al. 2006

Braz. oral res.

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This study assessed the surface softening and abrasive wear of eroded bovine enamel with or without the influence of toothbrushing. Five volunteers took part in this in situ study of 5 days. They wore acrylic palatal appliances containing 6 bovine enamel blocks divided in two rows with 3 blocks, which corresponded to the studied groups: erosion without toothbrushing (GI) and erosion with toothbrushing (GII). The blocks were subjected to erosion by immersion of the appliances in a cola drink for 10 minutes, 4 times a day. After that, no treatment was performed in one row (GI), whereas the other row was brushed (GII). The appliance was then replaced into the mouth. Enamel alterations were determined using profilometry and microhardness tests. Data were tested using paired Student's t test (p < 0.05). The mean wear values (µm) and percentage of superficial microhardness change (%SMHC) were respectively: GI -2.77 ± 1.21/91.61 ± 3.68 and GII -3.80 ± 0.91/58.77 ± 11.47. There was a significant difference in wear (p = 0.001) and %SMHC (p = 0.001) between the groups. It was concluded that the wear was more pronounced when associated to toothbrushing abrasion. However, toothbrushing promoted less %SMHC due to the removal of the altered superficial enamel layer. DESCRIPTORS: Tooth erosion; Tooth abrasion; Beverages. RESUMO:Este estudo avaliou o amolecimento superficial e o desgaste do esmalte bovino erodido com ou sem influência da escovação. Cinco voluntários fizeram parte deste estudo in situ de 5 dias. Eles usaram aparelhos palatinos de acrílico contendo 6 blocos de esmalte bovino divididos em 2 fileiras com 3 blocos, cada fileira correspondeu a um grupo em estudo: erosão sem escovação dentária (GI) e erosão seguida de escovação dentária (GII). Os blocos foram submetidos à erosão por imersão do aparelho em uma bebida à base de cola por 10 minutos, 4 vezes ao dia. Depois disso, nenhum tratamento foi realizado em uma fileira (GI), enquanto a outra fileira foi escovada (GII). Em seguida, o aparelho foi recolocado na boca. Alterações do esmalte foram determinadas por teste de perfilometria e microdureza. Os dados foram analisados usando-se teste t de Student (p < 0,05). O valor médio de desgaste (µm) e da porcentagem de perda de microdureza superficial (%PDS) foi respectivamente: GI -2,77 ± 1,21/91,61 ± 3,68 e GII -3,80 ± 0,91/58,77 ± 11,47. Houve uma diferença estatisticamente significante no desgaste (p = 0,001) e na %PDS (p = 0,001) entre grupos. Conclui-se que o desgaste foi mais pronunciado quando associado à abrasão pela escovação. Entretanto, a escovação promoveu menor %PDS, devido à remoção da camada de esmalte superficial alterada.

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Section: Discussionsupporting

confidence: 67%

Influence of toothbrushing on enamel softening and abrasive wear of eroded bovine enamel: an in situ study

Ríos

Honório²,

Magalhães³

et al. 2006

Braz. oral res.

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“…Low concentrations of carbamide peroxide promote varying degrees of surface porosity and structural change, depending on the bleaching agent 4,10,13,24 . The use of abrasive-containing dentifrice might result in enamel microwear 14,21 . The slight abrasion in this enamel may remove the superficial degraded layer and promote a "new" surface, even though, with high roughness values 14 .…”

Section: Discussionmentioning

confidence: 99%

“…The use of abrasive-containing dentifrice might result in enamel microwear 14,21 . The slight abrasion in this enamel may remove the superficial degraded layer and promote a "new" surface, even though, with high roughness values 14 . The bleached enamel surfaces treated with abrasive dentifrices with or without fluoride were markedly altered in our study.…”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of human dental enamel surface roughness bleached with 35% carbamide peroxide and submitted to abrasive dentifrice brushing

Worschech

Rodrigues

Martins

et al. 2003

Pesqui. Odontol. Bras.

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The aim of this in vitro study was to evaluate the surface roughness of human enamel bleached with 35% carbamide peroxide at different times and submitted to different superficial cleaning treatments: G1 - not brushed; G2 - brushed with fluoride abrasive dentifrice; G3 - brushed with a non-fluoride abrasive dentifrice; G4 - brushed without dentifrice. Sixty fragments of human molar teeth with 4 x 4 mm were obtained using a diamond disc. The specimens were polished with sandpaper and abrasive pastes. A perfilometer was used to measure roughness average (Ra) values of the initial surface roughness and at each 7-day-interval after the beginning of treatment. The bleaching was performed on the surface of the fragments for 1 hour a week, and the surface cleaning treatment for 3 minutes daily. The samples were stored in individual receptacles with artificial saliva. Analysis of variance and the Tukey test revealed significant differences in surface roughness values for G2 and G3, which showed an increase in roughness over time; G1 and G4 showed no significant roughness differences. The bleaching with 35% carbamide peroxide did not alter the enamel surface roughness, but when the bleaching treatment was performed combined with brushing with abrasive dentifrices, there was a significant increase in roughness values.

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“…For instance, the characteristics of demineralized primary enamel subjected to brushing with a dentifrice with or without fluoride. 6 Due to a lack of clear conclusions, this study was conducted to further evaluate the remineralization potential of caseinphosphopeptid-amorphous calcium fluoride phosphate (CPP-ACPF) paste on enamel lesions using SEM. The results emphasize the importance of using fluoride in combination with phosphate to treat caries at an early stage for permanent teeth; hence, an approach to managing comprehensive caries prevention is proposed to provide an initial healthy dentition for children and to contribute to the findings of in vitro studies in the dentistry field, which is still in need of supporting evidence.…”

Section: Using Scanning Electron Microscopy (Sem) Manymentioning

confidence: 99%

The Effect of Casein Phosphopeptide-amorphous Calcium Fluoride Phosphate on the Remineralization of Artificial Caries Lesions: An In Vitro Study

Nhu¹,

Hong²,

Le³

et al. 2017

J Dent Indones

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The studies on electron microstructure of the effect of the use of products that contain casein phosphopeptideamorphous calcium fluoride phosphate (CPP-ACPF) on enamel remineralization are still needed. It is important method to observe of the morphological changes of teeth in different conditions. Objective: To evaluate the remineralization potential of paste on enamel lesions using scanning electron microscopy (SEM). Methods: Sixty enamel specimens were prepared from extracted human premolars. The specimens were placed in a demineralizing solution for four days to produce artificial carious lesions. The specimens were then randomly assigned to two study groups: group A (control group) and group B. Group B was incubated in remineralizing paste (CPP-ACPF) for 30 minutes per day for 10 days. The control group received no intervention with remineralizing paste. All 60 specimens were stored in artificial saliva at 37 0 C. After remineralization, the samples were observed using SEM. Results: The statistical analysis showed a decrease in the lesion area between the demineralized and remineralized samples, but no significant difference was observed in the lesion depth for group B. There was a significant increase observed in both the lesion depth and lesion area for group A (p = 0.03). Conclusion: The results showed the capacity of CPP-ACPF in supplying calcium and phosphate to the enamel, decreasing the dissolution of the enamel surface and increasing the remineralization of the enamel surface.Keywords: CPP-ACPF, enamel, fluoride, remineralization, scanning electron microscopy How to cite this article: Ngoc VTN, Thuy PTH, Anh LQ, Son TM, Phuong NTT. The effect of casein phosphopeptideamorphous calcium fluoride phosphate on the remineralization of artificial caries lesions: an in vitro study. J Dent Indones. 2017;24(2): 45-49

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Microstructural analysis of demineralized primary enamel after in vitro toothbrushing

Cited by 14 publications

References 14 publications

Influence of toothbrushing on enamel softening and abrasive wear of eroded bovine enamel: an in situ study

Influence of toothbrushing on enamel softening and abrasive wear of eroded bovine enamel: an in situ study

In vitro evaluation of human dental enamel surface roughness bleached with 35% carbamide peroxide and submitted to abrasive dentifrice brushing

The Effect of Casein Phosphopeptide-amorphous Calcium Fluoride Phosphate on the Remineralization of Artificial Caries Lesions: An In Vitro Study

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