The demand for weight reduction, cost savings, more fuel efficiency, and reduced greenhouse gas has led to the increasing application of tailor welded blanks (TWBs) in the automotive industry. The purpose of this study is to investigate the strain rate effect on the tensile behavior of TWB consisting of dissimilar thickness sheets. Hence, a fiber laser was used for welding St14 steel sheets. The weld zone was examined using metallographic and microhardness tests. For investigating the tensile properties, uniaxial tensile tests were performed at strain rates ranging from 0.001 to 10 s−1. It has been found that the presence of Bainitic microstructure in the weld zone increases its hardness. Tensile test results indicate that yield strength and ultimate tensile strength of base metals and TWB enhanced with increasing strain rate. The yield and ultimate tensile strength of TWB are higher than base metals at different strain rates. As the strain rate increases, the total elongation of the TWB decreases, but in base metals, it decreases first and then increases at higher strain rates. In TWB and base metals, by increasing strain rate, uniform elongation decreases, but post-uniform elongation increases. For analysis of plastic deformation of thick and thin parts of TWB, indexes of limiting thickness ratio and difference of ratios (DR) were used. DR decreases with increasing strain rate, which indicates a decrease in the plastic deformation of the thick part of TWB and is consistent with the experimental findings.