Controlling the quality of cheese involves monitoring its taste, texture, stability and appearance. One method of doing so involves the analysis of cheese microstructure. This research investigated the use of confocal Raman microscopy for observing the microstructure of processed cheese including the distribution of additives therein. Reference Raman spectra were obtained for several additives commonly present in processed cheese including: trisodium citrate (TSC), sorbic acid, paprika and corn starch. Low and high spatial resolution Raman images were obtained from commercial and custom formulated processed cheeses. These were created using band integrals and principal component analysis scores values. Results of this investigation show that fat, protein, water, TSC, paprika and starch distributions can be imaged effectively using the many Raman imaging methods, including high spatial resolution Raman microscopy. Copyright © 2016 John Wiley & Sons, Ltd.