Microtiterplate phosphate assay based on luminescence quenching of a terbium complex amenable to decay time detection

Turel, Matejka; Duerkop, Axel; Yegorova, A. V.; Karasyov, Alexander A.; Scripinets, Yulia; Lobnik, Aleksandra

doi:10.1016/j.aca.2010.06.034

Cited by 8 publications

(6 citation statements)

References 47 publications

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“…In several previous studies, the effect of the buffer on the fluorescence intensity has been demonstrated [ 43 , 44 ]. The effect of buffer type on the RhB-EDA fluorescence response to Ag + ion has been tested.…”

Section: Resultsmentioning

confidence: 99%

Design and Investigation of Optical Properties of N-(Rhodamine-B)-Lactam-Ethylenediamine (RhB-EDA) Fluorescent Probe

Soršak

Valh

Urek

et al. 2018

Sensors

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This study presents chemical modification of a Rhodamine B (RhB) sensor probe by ethylenediamine (EDA), and investigation of its spectral as well as sensor properties to the various metals. The synthesised N-(Rhodamine-B)-lactam-ethylenediamine (RhB-EDA) fluorescent probe shows interesting optical sensor properties, and high sensitivity and selectivity to Ag+ ions among all the tested metal ions (K+, Mg2+, Cu2+, Ni2+, Fe2+, Pb2+, Na+, Mn2+, Li+, Al3+, Co2+, Hg2+, Sr2+, Ca2+, Ag+, Cd2+ and Zn2+), while the well-known Rhodamine B (RhB) fluorescent probe shows much less sensitivity to Ag+ ions, but high sensitivity to Fe2+ ions. The novel fluorescent sensor probe RhB-EDA has the capabilities to sense Ag+ ions up to µM ranges by using the fluorescence quenching approach. The probe displayed a dynamic response to Ag+ in the range of 0.43 × 10−3–10−6 M with a detection limit of 0.1 μM. The sensing system of an RhB-EDA novel fluorescent probe was optimised according to the spectral properties, effect of pH and buffer, photostability, incubation time, sensitivity, and selectivity. Since all the spectral and sensing properties were tested in green aqueous media, although many other similar sensor systems rely on organic solvent solutions, the RhB-EDA sensing probe may be a good candidate for measuring Ag+ ions in real-life applications.

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Section: Resultsmentioning

confidence: 99%

Design and Investigation of Optical Properties of N-(Rhodamine-B)-Lactam-Ethylenediamine (RhB-EDA) Fluorescent Probe

Soršak

Valh

Urek

et al. 2018

Sensors

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“…This method allows for the determination of phosphate anions in the micromolar range. Further tests indicate that the quenching mechanism is a static one [168].…”

Section: Quantitative Determination Of Lanthanide and Miscellaneous Ionsmentioning

confidence: 95%

Quinolone Complexes with Lanthanide Ions: An Insight into their Analytical Applications and Biological Activity

2020

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Quinolones comprise a series of synthetic bactericidal agents with a broad spectrum of activity and good bioavailability. An important feature of these molecules is their capacity to bind metal ions in complexes with relevant biological and analytical applications. Interestingly, lanthanide ions possess extremely attractive properties that result from the behavior of the internal 4f electrons, behavior which is not lost upon ionization, nor after coordination. Subsequently, a more detailed discussion about metal complexes of quinolones with lanthanide ions in terms of chemical and biological properties is made. These complexes present a series of characteristics, such as narrow and highly structured emission bands; large gaps between absorption and emission wavelengths (Stokes shifts); and long excited-state lifetimes, which render them suitable for highly sensitive and selective analytical methods of quantitation. Moreover, quinolones have been widely prescribed in both human and animal treatments, which has led to an increase in their impact on the environment, and therefore to a growing interest in the development of new methods for their quantitative determination. Therefore, analytical applications for the quantitative determination of quinolones, lanthanide and miscellaneous ions and nucleic acids, along with other applications, are reviewed here.

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“…Compared with other spectrophotometric methods for phosphates determination using organic molecules, Cys-capped AgNPs-CdS in this report for the detection of phosphates resulted in a much lower detection limit and a wider linear range. [22][23][24][25] In order to examine the selectivity of the method, the inuences of other anions on the Ce 3+ quenched uorescence of CdS, Cit-capped AgNPs-CdS and Cys-capped AgNPs-CdS were investigated. As shown in Fig.…”

Section: Effect Of Ce 3+ On the Uorescence Of Cys-capped Cds Qds In ...mentioning

confidence: 99%

Novel switchable sensor for phosphate based on the distance-dependant fluorescence coupling of cysteine-capped cadmium sulfide quantum dots and silver nanoparticles

Wang

Jiao

Zhu

et al. 2013

Analyst

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A novel switchable sensor was developed for the determination of phosphate based on Ce(3+) induced aggregation and phosphate triggered disaggregation of cysteine (Cys)-capped CdS quantum dots (QDs) and silver nanoparticles (AgNPs). The rare earth metal Ce(3+) could aggregate a mixture of QDs and AgNPs, which induced electron or energy transfer between CdS QDs and AgNPs and serious fluorescence quenching. However, phosphate dissociated the formed aggregation of CdS QDs and AgNPs, restoring the enhanced fluorescence of Cys-capped CdS triggered by AgNPs. Although, CdS QDs alone could also be used to detect phosphate through the aggregation-disaggregation mechanism adjusted by Ce(3+) and phosphate. It was found that the distance-dependent interaction between AgNPs and CdS QDs driven by Ce(3+) and phosphate could lead to enhanced quenching or enhancement of the fluorescence of Cys-capped CdS to form a more sensitive detection system for phosphate. The developed method was applied in the detection of phosphate in real water samples with acceptable and satisfactory results.

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Microtiterplate phosphate assay based on luminescence quenching of a terbium complex amenable to decay time detection

Cited by 8 publications

References 47 publications

Design and Investigation of Optical Properties of N-(Rhodamine-B)-Lactam-Ethylenediamine (RhB-EDA) Fluorescent Probe

Design and Investigation of Optical Properties of N-(Rhodamine-B)-Lactam-Ethylenediamine (RhB-EDA) Fluorescent Probe

Quinolone Complexes with Lanthanide Ions: An Insight into their Analytical Applications and Biological Activity

Novel switchable sensor for phosphate based on the distance-dependant fluorescence coupling of cysteine-capped cadmium sulfide quantum dots and silver nanoparticles

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