Microtubule Protein: Tubulin

Gozes, Illana; Littauer, Uriel Z.

doi:10.1111/j.1365-3083.1982.tb03770.x

Cited by 9 publications

(2 citation statements)

References 138 publications

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“…Microtubules are essential for cellular viability, maintaining cell shape and enabling cellular signaling, intracellular transport, mitosis, and cellular movement (Gozes and Littauer 1978;Gozes and Littauer 1982;Luduena 1998;Sharp et al 2000). The major subunit of microtubules, tubulin, is a heterodimer composed of two subunits α and β that exhibit cell-specific microheterogeneity (Gozes and Littauer 1978;Gozes and Sweadner 1981).…”

Section: Introductionmentioning

confidence: 99%

A Novel Method for Analyzing Mitochondrial Movement: Inhibition by Paclitaxel in a Pheochromocytoma Cell Model

Shprung

Gozes

2008

J Mol Neurosci

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A method was developed to assess mitochondrial movement in the living cell that is dependent, in part, on microtubule and/or associating protein interactions. The leader sequence from cytochrome-c was used to drive DsRed2 fluorescent proteins to accumulate in the mitochondria, thus enabling to follow mitochondrial (cytochrome-c's) movement. For calculating the percentage of mitochondrial movement, an image-processing program was used (ImageJ). Paclitaxel, an antitumor agent, is a potent microtubule-stabilizing agent that increases the stability of tubulin polymers, inhibiting mitosis and mitochondrial activity in dividing cells. Here, we tested whether paclitaxel inhibits mitochondrial movement in pheochromocytoma cells (a neuronal model, when tested in a differentiated state). While a 2-day exposure to paclitaxel resulted in cellular toxicity (measured as inhibition of mitochondrial activity), 2-3 h exposure to paclitaxel were sufficient to inhibit mitochondrial movement as assessed in 10-20-s imaging sessions in living cells. Mitotracker deep-red staining validated the staining obtained with DsRed2-cytochrome-c and identified intact mitochondria. Results showed a significant paclitaxel dose-dependent inhibition of mitochondrial movement. This new method should enable further assessment of microtubule-interacting drugs and other cytoskeletal components for their potential influence of mitochondrial movement as a test for activity and side effects.

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Section: Introductionmentioning

confidence: 99%

A Novel Method for Analyzing Mitochondrial Movement: Inhibition by Paclitaxel in a Pheochromocytoma Cell Model

Shprung

Gozes

2008

J Mol Neurosci

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“…Tubulin, the NAP binding protein in astrocytes ) exhibits specific isotypes in the developing brain and the neuritic processes (Gozes and Littauer 1978;Gozes and Sweadner 1981;Gozes and Barnstable 1982;Gozes and Littauer 1982;Joshi and Cleveland 1989;Banerjee et al 1990;Joshi and Cleveland 1990;Panda et al 1994). In higher eukaryotic cells, the heterodimer tubulin subunits are coded by a number of genes, e.g.…”

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confidence: 99%

Peptide neuroprotection through specific interaction with brain tubulin

Divinski

Holtser-Cochav

Vulih-Schultzman

et al. 2006

Journal of Neurochemistry

103

110

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This study aimed to identify the neuronal target for the potent neuroprotective peptide NAP. When added to pheochromocytoma cells (neuronal model), NAP was found in the intracellular milieu and was co-localized with microtubules. NAP induced neurite outgrowth and protected primary neurons against microtubule-associated ZnCl 2 toxicity. Rapid microtubule reorganization into distinct microtubules ensued after NAP addition to both pheochromocytoma cells and primary cerebral cortical neurons, but not to fibrobalsts. While binding neuronal tubulin and protecting pheochromocytoma cells against oxidative stress, NAP did not bind tubulin extracted from fibroblasts, nor did it protect those cells against oxidative stress. Affinity chromatography identified the brain-specific bIII-tubulin as a major NAP binding protein. Paclitaxel (a microtubule aggregating agent that interacts with b-tubulin) reduced NAP tubulin binding. Thus, the underlying mechanism for the neuroprotection offered by NAP is targeting neuronal microtubules that are essential for neuronal survival and function.

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From Concept to Potential Therapeutics: Neuroprotective Peptides

Gozes¹,

Tiong²

2007

Handbook of Neurochemistry and Molecular Neurobiology

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Microtubule Protein: Tubulin

Cited by 9 publications

References 138 publications

A Novel Method for Analyzing Mitochondrial Movement: Inhibition by Paclitaxel in a Pheochromocytoma Cell Model

A Novel Method for Analyzing Mitochondrial Movement: Inhibition by Paclitaxel in a Pheochromocytoma Cell Model

Peptide neuroprotection through specific interaction with brain tubulin

From Concept to Potential Therapeutics: Neuroprotective Peptides

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