Microvascular proliferation of brain metastases mimics glioblastomas in squash cytology

Gi, Toshihiro; Sato, Yuichiro; Tokumitsu, Takako; Yamashita, Atushi; Moriguchi-Goto, Sayaka; Takeshima, Hideo; Sato, Shinya; Asada, Yujiro

doi:10.1111/cyt.12405

Cited by 10 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6a ). This was of interest given the relationship between microvascular proliferation and high-grade glioma 35 , potentially suggesting localized cellular niches of more aggressive tumour features within metastases. Endothelial cell proliferation appeared to be suppressed via interactions with CD8 + T cells—an effect that was specific to BrM-cores (Fig.…”

Section: Single-cell Interaction Networkmentioning

confidence: 99%

Single-cell spatial immune landscapes of primary and metastatic brain tumours

Karimi

Maritan

et al. 2023

Nature

181

103

View full text Add to dashboard Cite

Single-cell technologies have enabled the characterization of the tumour microenvironment at unprecedented depth and have revealed vast cellular diversity among tumour cells and their niche. Anti-tumour immunity relies on cell–cell relationships within the tumour microenvironment1,2, yet many single-cell studies lack spatial context and rely on dissociated tissues3. Here we applied imaging mass cytometry to characterize the immunological landscape of 139 high-grade glioma and 46 brain metastasis tumours from patients. Single-cell analysis of more than 1.1 million cells across 389 high-dimensional histopathology images enabled the spatial resolution of immune lineages and activation states, revealing differences in immune landscapes between primary tumours and brain metastases from diverse solid cancers. These analyses revealed cellular neighbourhoods associated with survival in patients with glioblastoma, which we leveraged to identify a unique population of myeloperoxidase (MPO)-positive macrophages associated with long-term survival. Our findings provide insight into the biology of primary and metastatic brain tumours, reinforcing the value of integrating spatial resolution to single-cell datasets to dissect the microenvironmental contexture of cancer.

show abstract

Section: Single-cell Interaction Networkmentioning

confidence: 99%

Single-cell spatial immune landscapes of primary and metastatic brain tumours

Karimi

Maritan

et al. 2023

Nature

181

103

View full text Add to dashboard Cite

show abstract

“…Our results showed that tumor microvasculature of MLC had significantly larger microvessel diameters compared to PCNSLs, whereas microvessel size did not significantly differ between MLC tumors and GBMs nor between PCNSLs and GBMs. A histopathological study by Gi et al demonstrated that metastatic brain tumors, similar K to high-grade gliomas, are characterized by a large, thick, dilated glomeruloid microvasculature compared to normal brain tissue or malignant lymphomas [26], which could explain the non-significant difference in the mean vessel diameters between GBMs and MLCs. The lack of statistical significance in the comparison of microvessel size and density between GMBs and PCNSLs could be attributed to the small sample size but partially also to the fact that GBMs seem to alter their microvasculature as they evolve, with smaller tumors presenting with smaller vessels more similar to PCNSLs (which are less vascular and exhibit a characteristic angiocentric pattern in which tumor cells accumulate densely within and around blood vessels [27]).…”

Section: Discussionmentioning

confidence: 99%

Differentiation of Cerebral Neoplasms with Vessel Size Imaging (VSI)

et al. 2021

View full text Add to dashboard Cite

Purpose Cerebral neoplasms of various histological origins may show comparable appearances on conventional Magnetic Resonance Imaging (MRI). Vessel size imaging (VSI) is an MRI technique that enables noninvasive assessment of microvasculature by providing quantitative estimates of microvessel size and density. In this study, we evaluated the potential of VSI to differentiate between brain tumor types based on their microvascular morphology. Methods Using a clinical 3T MRI scanner, VSI was performed on 25 patients with cerebral neoplasms, 10 with glioblastoma multiforme (GBM), 8 with primary CNS lymphoma (PCNSL) and 7 with cerebral lung cancer metastasis (MLC). Following the postprocessing of VSI maps, mean vessel diameter (vessel size index, vsi) and microvessel density (Q) were compared across tumors, peritumoral areas, and healthy tissues. Results The MLC tumors have larger and less dense microvasculature compared to PCNSLs in terms of vsi and Q (p = 0.0004 and p < 0.0001, respectively). GBM tumors have higher yet non-significantly different vsi values than PCNSLs (p = 0.065) and non-significant differences in Q. No statistically significant differences in vsi or Q were present between GBMs and MLCs. GBM tumor volume was positively correlated with vsi (r = 0.502, p = 0.0017) and negatively correlated with Q (r = −0.531, p = 0.0007). Conclusion Conventional MRI parameters are helpful in differentiating between PCNSLs, GBMs, and MLCs. Additionally incorporating VSI parameters into the diagnostic protocol could help in further differentiating between PCNSLs and metastases and potentially between PCNSLs and GBMs. Future studies in larger patient cohorts are required to establish diagnostic cut-off values for VSI.

show abstract

“…Tumour angiogenesis has been revealed to be involved in mediating tumour growth and metastasis . The high level of microvessel density can be identified as an independent prognostic indictor for glioma patients . Ma et al identified SNHG15 as a novel lncRNA involved in the growth of glioma microvascular endothelial cells .…”

Section: Expression Pattern Functions and Clinical Potentials Of Snhmentioning

confidence: 99%

“…102,103 The high level of microvessel density can be identified as an independent prognostic indictor for glioma patients. 104,105 Ma et al identified SNHG15 as a novel lncRNA involved in the growth of glioma microvascular endothelial cells. 106 Their study demonstrated that SNHG15 was significantly increased in glioma-mediated human cerebral microvascular endothelial cells (hCMECs), which was cultured in the glioma conditioned medium (GCM) to simulate the glioma microenvironment.…”

Section: Gliomamentioning

confidence: 99%

LncRNA SNHG15: A new budding star in human cancers

Shuai

et al. 2019

Cell Proliferation

View full text Add to dashboard Cite

Objectives Long non‐coding RNAs (lncRNAs) represent an important group of non‐coding RNAs (ncRNAs) with more than 200 nucleotides in length that are transcribed from the so‐called genomic “dark matter.” Mounting evidence has shown that lncRNAs have manifested a paramount function in the pathophysiology of human diseases, especially in the pathogenesis and progression of cancers. Despite the exponential growth in lncRNA publications, our understanding of regulatory mechanism of lncRNAs is still limited, and a lot of controversies remain in the current lncRNA knowledge.The purpose of this article is to explore the clinical significance and molecular mechanism of SNHG15 in tumors. Materials & Methods We have systematically searched the Pubmed, Web of Science, Embase and Cochrane databases. We provide an overview of current evidence concerning the functional role, mechanistic models and clinical utilities of SNHG15 in human cancers in this review. Results Small nucleolar RNA host gene 15 (SNHG15), a novel lncRNA, is identified as a key regulator in tumorigenesis and progression of various human cancers, including colorectal cancer (CRC), gastric cancer (GC), pancreatic cancer (PC) and hepatocellular carcinoma (HCC). Dysregulation of SNHG15 has been revealed to be dramatically correlated with advanced clinicopathological factors and predicts poor prognosis, suggesting its potential clinical value as a promising biomarker and therapeutic target for cancer patients. Conclusions LncRNA SNHG15 may serve as a prospective and novel biomarker for molecular diagnosis and therapeutics in patients with cancer.

show abstract

Microvascular proliferation of brain metastases mimics glioblastomas in squash cytology

Abstract: The vascular features of metastatic brain tumours are similar to those of glioblastomas, suggesting that these microvascular proliferations contribute to the progression of metastatic tumours.

Cited by 10 publications

References 20 publications

Single-cell spatial immune landscapes of primary and metastatic brain tumours

Single-cell spatial immune landscapes of primary and metastatic brain tumours

Differentiation of Cerebral Neoplasms with Vessel Size Imaging (VSI)

LncRNA SNHG15: A new budding star in human cancers

Contact Info

Product

Resources

About