Microvessel networks in pre-formed in artificial clinical grade dermal substitutes in vitro using cells from haematopoietic tissues

Athanassopoulos, A.; Tsaknakis, Grigorios; Newey, Sarah E.; Harris, Adrian L.; Kean, Jennifer; Tyler, Michael; Watt, Suzanne M.

doi:10.1016/j.burns.2011.12.019

Cited by 24 publications

(34 citation statements)

References 42 publications

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“…We have previously isolated and demonstrated the difference in functional capacity of adult PB and CB ECFC-derived cells5. These cells have similar cell surface phenotypes as determined by the FACS analyses and by their morphologies (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 94%

“…Nine CB and 9 PB donations were collected and used for the experimental studies. Human ECFC-derived cells were next generated by culturing the CB and PB mononuclear cells (2 × 10 7 cells in 6 well collagen coated plates) in 4 mls complete EGM-2 media (Lonza Biologics, Cambridge, England) supplemented with 10% (v/v) Hyclone heat inactivated fetal bovine serum (FBS; ThermoScientific, Waltham, MA, USA)510. Endothelial colonies, which formed by 21 days of culture, were selected using cloning rings and those from individual CB or PB units were pooled and then passaged in the above medium510.…”

Section: Methodsmentioning

confidence: 99%

“…These ECFCs generate de novo vessels in vitro and in immunodeficient murine models in vivo, and their functional potency correlates with their proliferative potential12345678910111213141516171819. In pre-clinical models, ECFCs are reported to enhance the patency of implanted vascular grafts and to improve outcomes in ischemic diseases, such as stroke, myocardial infarction and limb ischemia202122232425262728293031.…”

mentioning

confidence: 99%

“…In the human, ECFCs from healthy perinatal CB are significantly more frequent than those found circulating in healthy adult PB and have a higher proliferative capacity3561115161819. Thus, defining strategies to enhance the content of these cells in the adult, while retaining their vasculogenic/angiogenic functions would be beneficial.…”

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confidence: 99%

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miR-193a-3p interaction with HMGB1 downregulates human endothelial cell proliferation and migration

Khoo

Roubelakis

Schrader

et al. 2017

Sci Rep

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Circulating endothelial colony forming cells (ECFCs) contribute to vascular repair where they are a target for therapy. Since ECFC proliferative potential is increased in cord versus peripheral blood and to define regulatory factors controlling this proliferation, we compared the miRNA profiles of cord blood and peripheral blood ECFC-derived cells. Of the top 25 differentially regulated miRNAs selected, 22 were more highly expressed in peripheral blood ECFC-derived cells. After validating candidate miRNAs by q-RT-PCR, we selected miR-193a-3p for further investigation. The miR-193a-3p mimic reduced cord blood ECFC-derived cell proliferation, migration and vascular tubule formation, while the miR-193a-3p inhibitor significantly enhanced these parameters in peripheral blood ECFC-derived cells. Using in silico miRNA target database analyses combined with proteome arrays and luciferase reporter assays of miR-193a-3p mimic treated cord blood ECFC-derived cells, we identified 2 novel miR-193a-3p targets, the high mobility group box-1 (HMGB1) and the hypoxia upregulated-1 (HYOU1) gene products. HMGB1 silencing in cord blood ECFC-derived cells confirmed its role in regulating vascular function. Thus, we show, for the first time, that miR-193a-3p negatively regulates human ECFC vasculo/angiogenesis and propose that antagonising miR-193a-3p in less proliferative and less angiogenic ECFC-derived cells will enhance their vasculo/angiogenic function.

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Section: Resultsmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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miR-193a-3p interaction with HMGB1 downregulates human endothelial cell proliferation and migration

Khoo

Roubelakis

Schrader

et al. 2017

Sci Rep

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“…[47] Research into this is worth pursuing, given the slow repair rates of current tissue, [48] because accelerating the appearance of blood vessels, neodermis, and epithelialization can deliver enhanced skin repair. [49] …”

Section: Concluding Commentsmentioning

confidence: 99%

Perspectives on the Molecular and Biological Implications of Tropoelastin in Human Tissue Elasticity

Weiss¹

2016

Aust. J. Chem.

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The elasticity of a range of vertebrate and particularly human tissues depends on the dynamic and persistent protein elastin. This elasticity is diverse, and comprises skin, blood vessels, and lung, and is essential for tissue viability. Elastin is predominantly made by assembling tropoelastin, which is an asymmetric 20-nm-long protein molecule. This overview considers tropoelastin's molecular features and biological interactions in the context of its value in tissue repair.

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Tropoelastin Incorporation into a Dermal Regeneration Template Promotes Wound Angiogenesis

Wang

Mithieux

Kong

et al. 2014

Adv Healthcare Materials

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Severe burn injury results in substantial skin loss and cannot be treated by autografts. The Integra Dermal Regeneration Template (IDRT) is the leading synthetic skin substitute because it allows for wound bed regeneration and wound healing. However, all substitutes suffer from slow blood vessel ingrowth and would benefit considerably from enhanced vascularization to nurture tissue repair. It is shown here that by incorporating the human elastic protein tropoelastin into a dermal regeneration template (TDRT) we can promote angiogenesis in wound healing. In small and large animal models comprising mice and pigs, the hybrid TDRT biomaterial and IDRT show similar contraction to autografts and decrease wound contraction compared to open wounds. In mice, TDRT accelerates early stage angiogenesis by 2 weeks, as evidenced by increased angiogenesis fluorescent radiant efficiency in live animal imaging and the expression of endothelial cell adhesion marker CD146. In the pig, a full thickness wound repair model confirms increased numbers of blood vessels in the regenerating areas of the dermis closest to the hypodermis and immediately below the epidermis at 2 weeks post-surgery. It is concluded that including tropoelastin in a dermal regeneration template has the potential to promote wound repair through enhanced vascularization.

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Microvessel networks in pre-formed in artificial clinical grade dermal substitutes in vitro using cells from haematopoietic tissues

Cited by 24 publications

References 42 publications

miR-193a-3p interaction with HMGB1 downregulates human endothelial cell proliferation and migration

miR-193a-3p interaction with HMGB1 downregulates human endothelial cell proliferation and migration

Perspectives on the Molecular and Biological Implications of Tropoelastin in Human Tissue Elasticity

Tropoelastin Incorporation into a Dermal Regeneration Template Promotes Wound Angiogenesis

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