Microwave assisted antibacterial action of Garcinia nanoparticles on Gram-negative bacteria

Abstract

Owing to the existence of the outer membrane barrier, most antibacterial agents cannot penetrate Gram-negative bacteria and are ineffective. Here, we report a general method for narrow-spectrum antibacterial Garcinia nanoparticles that can only be effective to kill Gram-positive bacteria, to effectively eliminate Gram-negative bacteria by creating transient nanopores in bacterial outer membrane to induce drug entry under microwaves assistance. In vitro, under 15 min of microwaves irradiation, the antibacterial efficiency of Garcinia nanoparticles against Escherichia coli can be enhanced from 6.73% to 99.48%. In vivo, MV-assisted GNs can effectively cure mice with bacterial pneumonia. The combination of molecular dynamics simulation and experimental results reveal that the robust anti-E. coli effectiveness of Garcinia nanoparticles is attributed to the synergy of Garcinia nanoparticles and microwaves. This work presents a strategy for effectively treating both Gram-negative and Gram-positive bacteria co-infected pneumonia using herbal medicine nanoparticles with MV assistance as an exogenous antibacterial auxiliary.

Potential Application of Luteolin as an Active Antibacterial Composition in the Development of Hand Sanitizer Products

“…The results demonstrated that LUT inhibited the growth of E. coli and S. aureus . Notably, the antimicrobial activity of LUT was higher against S. aureus than that of E. coli , indicating that the differences in cell wall structures between Gram-positive and Gram-negative microorganisms could affect the antibacterial effects of this compound [ 15 , 16 , 17 , 18 ].…”

Stimuli-Activable Metal-Bearing Nanomaterials and Precise On-Demand Antibacterial Strategies

“…External stimuli that can activate antimicrobial actions include light, magnets, microwaves, ultrasound, etc . − Different metal-bearing nanomaterials possess distinct responses to these external stimuli. For example, semiconductor nanomaterials ( e.g.…”

“…As mentioned above, SAMNs are a series of nanomaterials that remain dormant in the absence of external stimuli and selectively turn on when subjected to external stimuli, including light, magnetism, microwave, ultrasound, etc . − The light triggered actions can be further divided into photodynamic and photothermal antibacterial therapies. The magnet triggered actions include magnetothermal and magnetophysical antibacterial modes.…”

“…This type of materials remains “dormant” in the absence of external stimuli and selectively “turns on” when subjected to various stimuli . Hence, they can be used to establish “on-demand antibacterial” strategies to achieve the control of antibacterial time and space, thus reducing the possible harmful side effects on normal tissues and cells. − Such on-demand strategies will also avoid failure and the waste of materials to achieve cost-effectiveness.…”

Sea urchin-like Bi2S3/curcumin heterojunction rapidly kills bacteria and promotes wound healing under near-infrared light

“…The previous studies have reported tea nanoclusters as β-lactamase inhibitors for the treatment of multidrug-resistant bacterial pneumonia, 29 as well as the use of tea nanodots containing eight tea catechins with an average size of about 3 nm to kill MRSA and treat MRSA-H1N1 pneumonia. 30 In addition, Garcinia nanoparticles can effectively kill Gram-negative bacteria under microwave-assisted action, 31 and there is also MoS 2 /FeS polarization with Rhein interface to enhance microwave-catalyzed treatment of bacterial infections of osteomyelitis. 32 Similarly, under the synergistic excitation of near-infrared light and ultrasound, CuS forms a tight interface with curcumin to achieve rapid broad-spectrum bactericidal action.…”