Microwave-assisted extraction of antioxidant compounds from by-products of Turkish hazelnut (Corylus avellana L.) using natural deep eutectic solvents: Modeling, optimization and phenolic characterization

Bener, Mustafa; Şen, Furkan Burak; Önem, Ayşe Nur; Bekdeşer, Burcu; Çelik, Saliha Esin; Lalikoğlu, Melisa; Aşçı, Yavuz Selim; Çapanoğlu, Esra; Apak, Reşat

doi:10.1016/j.foodchem.2022.132633

“…The influence of viscosity on the extraction of DNA 17 and anthraquinones 18 have been investigated earlier. Similar investigations on effect of density on extraction of antioxidant compounds 19 and crocins 20 have elucidated its dependence on the selectivity of DES. Hence investigating the physical properties of NADES that influence their task-specific nature could enable a selective environment for extractive fermentation of naringinase 21 , 22 .…”

Section: Introductionmentioning

confidence: 77%

Simultaneous production and sustainable eutectic mixture based purification of narringinase with Bacillus amyloliquefaciens by valorization of tofu wastewater

Balaraman

¹

,

Purushotaman

²

,

Chandramouliswaran

³

et al. 2022

Sci Rep

5

0

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The current investigation is being executed for sustainable one-pot production and purification of naringinase using natural deep eutectic solvent-based extractive fermentation. Five natural deep eutectic solvents were prepared and their physicochemical properties were determined as a function of temperature. Tofu wastewater was used as a low-cost substrate for naringinase production and simultaneous in-situ purification of the enzyme was accomplished by employing NADES. Optimal conditions of influential factors like concentrations of NADES (74.5% w/w), Na2SO4 (15% w/v) and tofu wastewater (1.5% w/w) resulted in an effective yield of naringinase (249.6 U/ml). Scale-up of naringinase production with a 3 l custom made desktop bioreactor was accomplished and effective regeneration of NADES was established. NADES exhibits selectivity during extraction even after the fifth cycle proving it to be tailor-made. The resulting active enzyme was quantified by size exclusion chromatography (736.85 U/mg). Ultrapure enzyme fraction was obtained with anion exchange chromatography yielding maximum purity of (63.2 U/ml) and specific naringinase activity of (3516 U/mg). The in-vitro debittering activity of the resulting ultrapure enzyme fraction was determined with grape juice resulting in naringin and limonin removal of [23.4% (w/w)] and [64.3% (w/w)] respectively.

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“…This observation highlights that extended exposure of BMP to ultrasound can trigger the degradation of phenolics and flavonoids [13]. The deterioration of these compounds results in the reduction in ABTS, DPPH, and OH of BMP extracts [40]. As a result, fifteen minutes was an appropriate extraction time to obtain the highest extraction yield of bioactive compounds from BMP by using NADES8 combined with UAE.…”

Section: Effect Of Timementioning

confidence: 86%

“…The appropriate NADES were chosen from section 2.3 for use in one-factor experiments. Onefactor experiments were performed to investigate the influence of the technical parameters, including liquid-to-solid ratios (LSR (NADES:BMP), 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 ml/g), water content (10,20,30,40, and 50%, g/g), temperature (30,40,50,60, 70, and 80°C), and retention time (5,10,15,30,50,70, and 90 min) on the efficiency of UAE process. Briefly, the quantified amount of BMP was added to a 100-ml amber glass bottle, 25 ml of NADES was poured into an amber glass bottle, and then sonicated under selected conditional ranges.…”

Section: One-factor Experiments For Uae Combined With Nadesmentioning

confidence: 99%

Green extraction of phenolics and flavonoids from black mulberry fruit using natural deep eutectic solvents: optimization, kinetics and surface morphology

Vo

¹

,

Pham

²

,

Weina

³

et al. 2022

Preprint

0

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This study deployed ultrasonic-assisted extraction (UAE), combined with natural deep eutectic solvents (NADES), to extract phenolics and flavonoids from the black mulberry fruit, and the antioxidant activity was examined. The extraction yields of NADES-based UAE were assessed based on total phenolic and flavonoid contents (TPC and TFC, respectively). NADES produced from choline chloride-lactic acid at a 1:2 molar ratio had the highest extraction yield. One-factor experiments evaluated the effect of UAE conditions (liquid-to-solid ratio, water content in NADES, temperature, and time) on TPC, TFC, and antioxidant activity. Response surface methodology with the Box-Behnken design model optimized the NADES-based UAE process based on response (TPC, TFC, ABTS, OH, and DPPH). The optimal conditions for the NADES-based UAE process were 70 ml/g of LSR, 38.9% water content in NADES, 67.9°C, and 24.2 min of retention time. The predicted values of the Box-Behnken design were compatible with the experimental results. Moreover, scanning electron microscopy was used to survey the surface of black mulberry fruit with and without sonication. This study proposes an efficient and green method for extracting bioactive compounds from the black mulberry fruit.

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“…The appropriate NADES were chosen from section 2.3 for use in one-factor experiments. One-factor experiments were performed to investigate the in uence of the technical parameters, including liquid-to-solid ratios (LSR (NADES:BMP), 10-100 ml/g), water content (10,20,30,40, and 50%, g/g), temperature (30,40,50, 60, 70, and 80°C), and retention time (5,10,15,30,50,70, and 90 min) on the e ciency of UAE process. Brie y, the quanti ed amount of BMP was added to a 100-ml amber glass bottle, 25 ml of NADES was poured into an amber glass bottle, and then sonicated under selected conditional ranges.…”

Section: One-factor Experiments For Uae Combined With Nadesmentioning

confidence: 99%

Green extraction of phenolics and flavonoids from black mulberry fruit using natural deep eutectic solvents: optimization and surface morphology

Vo

¹

,

Pham

²

,

Weina

³

et al. 2023

Preprint

0

View full text Add to dashboard Cite

This study deployed ultrasonic-assisted extraction (UAE), combined with natural deep eutectic solvents (NADES), to extract phenolics and flavonoids from the black mulberry fruit, and the antioxidant activity was examined. The extraction yields of NADES-based UAE were assessed based on total phenolic and flavonoid contents (TPC and TFC, respectively). NADES produced from choline chloride-lactic acid at a 1:2 molar ratio had the highest extraction yield. One-factor experiments evaluated the effect of UAE conditions (liquid-to-solid ratio, water content in NADES, temperature, and time) on TPC, TFC, and antioxidant activity. Response surface methodology with the Box-Behnken design model optimized the NADES-based UAE process based on response (TPC, TFC, ABTS, OH, and DPPH). The optimal conditions for the NADES-based UAE process were 70 ml/g of LSR, 38.9% water content in NADES, 67.9°C, and 24.2 min of retention time. The predicted values of the Box-Behnken design were compatible with the experimental results. Moreover, scanning electron microscopy was used to survey the surface of black mulberry fruit with and without sonication. This study proposes an efficient and green method for extracting bioactive compounds from black mulberry fruits.

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Microwave-assisted extraction of antioxidant compounds from by-products of Turkish hazelnut (Corylus avellana L.) using natural deep eutectic solvents: Modeling, optimization and phenolic characterization

Cited by 89 publications

References 65 publications

Simultaneous production and sustainable eutectic mixture based purification of narringinase with Bacillus amyloliquefaciens by valorization of tofu wastewater

Simultaneous production and sustainable eutectic mixture based purification of narringinase with Bacillus amyloliquefaciens by valorization of tofu wastewater

Green extraction of phenolics and flavonoids from black mulberry fruit using natural deep eutectic solvents: optimization, kinetics and surface morphology

Green extraction of phenolics and flavonoids from black mulberry fruit using natural deep eutectic solvents: optimization and surface morphology

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