Microwave assisted green synthesis of Hydroxyapatite nanorods using <i>Moringa oleifera</i> flower extract and its antimicrobial applications

Kalaiselvi, V.; Mathammal, R.; Vijayakumar, Sekar; Vaseeharan, Baskaralingam

doi:10.1016/j.ijvsm.2018.08.003

Cited by 78 publications

(25 citation statements)

References 25 publications

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“…In support of our study, Kalaiselvi et al (2018) synthesized HA nanorods using the flower extract of Moringa oleifera and observed the potent antibacterial activity on Gram-positive bacteria ( Bacillus subtilis , Monococcus luteus , and S. aureus ) and Gram-negative bacteria ( Klebsiella pneumoniae , Pseudomonas aeruginosa , and Salmonella paratyphi ). Likewise, Scatolini et al (2018) tested biomimetic HA with different Brazilian propolis and noticed potent antibacterial activity on S. aureus ATCC 25923 in the range of >12.5 to >100 μg/ml for MIC and >206.7–800 μg/ml for MBC.…”

Section: Resultssupporting

confidence: 68%

“…Insight of the aforementioned demerits, biomimetic of HA composite with potent antibacterial and anti-inflammatory bioresources could be highly preferred and recommended over chemical substances for orthopedic implants. In the current scenario, plant sources are highly preferred due to their non-toxicity and eco-friendliness (Kalaiselvi et al, 2018; Phatai et al, 2019). Therefore, the biomimetic of HA with plant sources could be highly appropriate and satisfactory.…”

Section: Introductionmentioning

confidence: 99%

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Presentation of Antibacterial and Therapeutic Anti-inflammatory Potentials to Hydroxyapatite via Biomimetic With Azadirachta indica: An in vitro Anti-inflammatory Assessment in Contradiction of LPS-Induced Stress in RAW 264.7 Cells

Nagaraj

Samiappan

2019

Front. Microbiol.

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In the present study, for the first time, biomimetization of hydroxyapatite (HA) with Azadirachta indica (AI) was proposed and established its antioxidant, antibacterial, and anti-inflammatory potential on lipopolysaccharide (LPS). The ethanolic extract of AI was found rich with phenolics and flavonoids, and determined their concentration as 8.98 ± 1.41 mg gallic acid equivalents/g and 5.46 ± 0.84 mg catechin equivalents/g, respectively. The HA was prepared by sol-gel method from calcium nitrate tetrahydrate and orthophosphoric acid, and successfully biomimetization was performed with ethanolic extract of AI. The FTIR analysis settled that as-synthesized HA-AI composite was comprised of both HA and AI. The XRD pattern and Zeta potential revealed that the HA-AI composite was crystalline and negative in charge (−24.0 mV). The average-size distribution, shape, and size of the HA-AI composite was determined as 238.90 d.nm, spherical, and 117.90 nm from size distribution, SEM, and HR-TEM analysis, respectively. The SEM-EDX concluded that the HA-AI composite was comprised of elements of HA as well as AI. The HA-AI composite presented potential antioxidant activity and its EC50 values (dose required to inhibit about half of the radicals) for ABTS and DPPH assays were determined as 115.72 ± 2.33 and 128.51 ± 1.04 μg/ml, respectively. The HA-AI composite showed potent antibacterial activity, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) towards S. aureus (ATCC 700699) and E. coli (ATCC 10536) were correspondingly determined as 266.7 ± 28.87 and 600.0 ± 50.0 μg/ml, and 400.0 ± 86.6 and 816.7 ± 76.38 μg/ml. Most importantly, HA-AI composite presented the potential antiinflammatory response toward lipopolysaccharide (LPS) in RAW 264.7 cells. The dose of 250 μg/ml of HA-AI composite has shown optimum protection against LPS-induced stress (1 μg/ml) by scavenging oxidants and regulating mitochondrial membrane potential (MMP), inflammatory and apoptotic factors. Thus, this study concluded that the impartation of potential biofunctional features to HA from plant sources through biomimetic approach is much beneficial and could find potential application in dentistry and orthopedic.

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Section: Resultssupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Presentation of Antibacterial and Therapeutic Anti-inflammatory Potentials to Hydroxyapatite via Biomimetic With Azadirachta indica: An in vitro Anti-inflammatory Assessment in Contradiction of LPS-Induced Stress in RAW 264.7 Cells

Nagaraj

Samiappan

2019

Front. Microbiol.

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“…However, the % of carbonate content in the Hap samples was calculated from the FT-IR data as described in the following §3. Next, to examine the effect of mesh size together with the calcination temperature on the crystallographic properties of Hap, the crystallite size (D c ), crystallinity degree (Xc) and crystallinity index (CI), dislocation density (δ) and micro-strain (ε) of all the Hap samples were calculated using well-established equations [30,31] and summarized in table 1.…”

Section: Characterization Of Source Materialsmentioning

confidence: 99%

Coupled effect of particle size of the source materials and calcination temperature on the direct synthesis of hydroxyapatite

et al. 2021

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We report the effect of controlled particle size (obtained by using 80, 100, 120, 140 and 200 mesh) of the source materials on the synthesis of a well-known biomaterial, hydroxyapatite (Hap). In addition to this, we have also mapped the consequence of applied temperature (700°C, 800°C and 900°C) on the crystallographic properties and phase composition of the obtained Hap. Nevertheless, although with Hap, in each case, β-tricalcium phosphate (β-TCP) was registered as the secondary phase the ANOVA test revealed that the results of the crystallographic parameters are significantly different for the applied sintering temperature 700°C and 800°C ( p < 0.05), while the data obtained for calcination temperature 800°C are not significantly different from that acquired at 900°C ( p > 0.05). Fourier transform infrared spectrophotometer data ensured that, irrespective of mesh size and calcination temperature, the synthesized Hap samples were of carbonated apatite with B-type substitution. Interestingly, for all cases, the % of carbonate content was below the maximum limit (8%) of the CO 3 2 − ion present in bone tissue hydroxyapatite.

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“…Most of the synthetic methods require toxic organic solvents for the preparation of HAp. To minimize the usage of organic solvents, HAp nanoparticles have been synthesized using green extract as a template medium, which is highly advantageous because of its low cost, ecofriendly, and less toxicity 22,23 . On the other hand, several attempts have been made for the fabrication of biofunctional nanoparticles through simple and efficient synthesis method with specific morphological changes.…”

Section: Introductionmentioning

confidence: 99%

“…To minimize the usage of organic solvents, HAp nanoparticles have been synthesized using green extract as a template medium, which is highly advantageous because of its low cost, ecofriendly, and less toxicity. 22,23 On the other hand, several attempts have been made for the fabrication of biofunctional nanoparticles through simple and efficient synthesis method with specific morphological changes. The mediated synthesis using plant extract has gained emerging interest as an active research area in nanotechnology.…”

mentioning

confidence: 99%

An innovative Azadirachta indica gum‐mediated synthesis of cocoon‐shaped nano‐AgHAp from Lamellidens marginalis shells

Ponnusamy

Sadhasivam

Kavitha

et al. 2020

Int J Applied Ceramic Tech

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In this work, the evaluations of noble nanoparticles for the structural and morphological characteristics are focused. The control of desired particles size and morphology for hydroxyapatite (HAp) and Ag‐substituted hydroxyapatite (AgHAp) derived from Lamellidens marginalis shells using Azadirachta Indica (AI) gum as a potential surfactant for the synthesis of stable nanoparticles are reported. The morphological change with respect to the concentration of AI gum is analyzed. The functional group (FTIR) and crystallographic (XRD) characterization of the HAp and AgHAp nanoparticles confirm the presence of HAp with desired apatite functional peaks. The morphological evaluation (FE‐SEM) exhibited the formation of cocoon‐shaped nanoparticles for the AI gum‐medicated synthesis. Higher AI gum concentration reduces the particle size along with the formation of unique surface morphology. The average diameter of the synthesized AgHAp nanoparticles was found to be ≤30 nm which is revealed from HR‐TEM. The bacterial investigation against bacterial strains substantiates the higher resistance of bacterial growth for Staphylococcus aureus was observed than Escherichia coli for the AgHAp particles. Hence, embedding silver nanoparticles in the HAp is an efficient approach to enhance the long‐term antibacterial effect of the orthopedic and dental applications.

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Microwave assisted green synthesis of Hydroxyapatite nanorods using Moringa oleifera flower extract and its antimicrobial applications

Abstract: Graphical abstract

Cited by 78 publications

References 25 publications

Presentation of Antibacterial and Therapeutic Anti-inflammatory Potentials to Hydroxyapatite via Biomimetic With Azadirachta indica: An in vitro Anti-inflammatory Assessment in Contradiction of LPS-Induced Stress in RAW 264.7 Cells

Presentation of Antibacterial and Therapeutic Anti-inflammatory Potentials to Hydroxyapatite via Biomimetic With Azadirachta indica: An in vitro Anti-inflammatory Assessment in Contradiction of LPS-Induced Stress in RAW 264.7 Cells

Coupled effect of particle size of the source materials and calcination temperature on the direct synthesis of hydroxyapatite

An innovative Azadirachta indica gum‐mediated synthesis of cocoon‐shaped nano‐AgHAp from Lamellidens marginalis shells

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