Microwave irradiation influences on the state of human cell nuclei

Shckorbatov, Y. G.; Grigoryeva, N.N.; Shakhbazov, V.G.; Grabina, V.A.; Bogoslavsky, A. M.

doi:10.1002/(sici)1521-186x(1998)19:7<414::aid-bem2>3.0.co;2-4

Cited by 40 publications

(20 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As is known, heterochromatin is mainly the inactive part of chromatin with low transcriptional activity (Lewin, 2004). Our data on microwave-induced heterochromatin granule formation in human fibroblasts were in a good agreement with previous data in human buccal epithelium cells (Shckorbatov et al, 1998;. The biological consequences of electromagnetic irradiation in connection to heterochromatin granule formation may be the following.…”

Section: Resultssupporting

confidence: 91%

See 1 more Smart Citation

Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts

Shckorbatov¹,

Pasiuga²,

Goncharuk

et al. 2010

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Abstract:To investigate the influence of microwave radiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization, linear and left-handed and right-handed elliptically polarized, were examined. Experimental conditions were: frequency (f) 36.65 GHz, power density (P) at the surface of exposed object 1, 10, 30, and 100 µW/cm 2 , exposure time 10 s. Human fibroblasts growing in a monolayer on a cover slide were exposed to microwave electromagnetic radiation. The layer of medium that covered cells during microwave exposure was about 1 mm thick. Cells were stained immediately after irradiation by 2% (w/v) orcein solution in 45% (w/v) acetic acid. Experiments were made at room temperature (25 °C), and control cell samples were processed in the same conditions. We assessed heterochromatin granule quantity (HGQ) at 600× magnification. Microwave irradiation at the intensity of 1 µW/cm 2 produced no effect, and irradiation at the intensities of 10 and 100 µW/cm 2 induced an increase in HGQ. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized radiation revealed more biological activity than the left-handed polarized one.

show abstract

Section: Resultssupporting

confidence: 91%

“…In our previous work we showed that microwaves induce an increase of heterochromatinization in the nuclei of human buccal epithelium cells (Shckorbatov et al, 1998). This phenomenon is related to the state of polarization of the electromagnetic wave (Shckorbatov et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts

Shckorbatov¹,

Pasiuga²,

Goncharuk

et al. 2010

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

show abstract

“…The effect of chromatin condensation under stress conditions has been understood since the 1970's [18]. However, microwaveinduced chromatin condensation was not shown for the first time until 1998 [19].…”

Section: Resultsmentioning

confidence: 99%

Electromagnetic field effects on Artemia hatching and chromatin state

et al. 2010

View full text Add to dashboard Cite

, exposure time-10s.Afterincubationofthecystsfor48hoursinseawaterthehatchingpercentageofArtemiafromexposedcystswas higherthanincontrols.Thenumberofheterochromatingranuleswassignificantlyhigherinthenauplia(newbornlarvaeofArtemia) developedfromcyststhathadbeenexposedtomagneticand electromagneticfields.Thedataobtaineddemonstratean increase in percentage hatching of Artemia cysts after treatment with magnetic and electromagnetic fields and chromatin condensation in nauplia. We have also shown different effects of right-handed and left-handed rotating magnetic fields on these processes.

show abstract

“…For instance, the negative electric charge of plant cell nuclei rises after treatment of plants with the phytohormone gibberellin, which stimulates biosynthetic processes, and decreases immediately after heat shock, which inhibits biosynthetic processes . The electronegativity of human cell nuclei also decreases under the in¯uence of microwave radiation [Shckorbatov et al, 1998]. Negative electric charge of chromatin and nucleoli in cell nuclei increases in the process of repair of heat shock induced injuries [Shckorbatov and Shakhbazov, 1983].…”

Section: Introductionmentioning

confidence: 82%

“…For instance, no signi®cant changes in membrane permeability were reported after microwave treatment of cells, but in this case ENN decrease and chromatin condensation were observed [Shckorbatov et al, 1998]. The results of the present study show the lowest ENN level in cells of aged donors with the most permeable cell membranes.…”

Section: V/cm At Whichmentioning

confidence: 99%

Modification of electrokinetic properties of nuclei in human buccal epithelial cells by electric fields

Shckorbatov

Shakhbazov

Rudenko

2001

Bioelectromagnetics

View full text Add to dashboard Cite

The influence of an alternating (50 Hz) electric field (5--110 V/cm) on the state of human buccal epithelium cells was studied by the methods of intracellular microelectrophoresis, heterochromatin staining with orcein, and indigo carmine staining for viability and membrane integrity evaluations. Electric field exposure induced an increase in electrophoretic mobility of cell nuclei, decreased numbers of heterochromatin granules near the inner membrane of cell nucleus, and induced cell membrane damage; but cell viability was conserved. Nuclear and cell membrane properties varied with electric field strength and age of the donors. The data obtained are interpreted as evidence of electric field induced activation of the functional state of nuclei.

show abstract

Microwave irradiation influences on the state of human cell nuclei

Cited by 40 publications

References 18 publications

Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts

Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts

Electromagnetic field effects on Artemia hatching and chromatin state

Modification of electrokinetic properties of nuclei in human buccal epithelial cells by electric fields

Contact Info

Product

Resources

About