Modification of electrokinetic properties of nuclei in human buccal epithelial cells by electric fields

Shckorbatov, Y. G.; Shakhbazov, V.G.; Rudenko, Andrii

doi:10.1002/1521-186x(200102)22:2<106::aid-bem1013>3.0.co;2-2

Cited by 3 publications

(2 citation statements)

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“…In recent years, the findings concerning the MF-dependence of the FL cell and nuclear volumes 4,83 were supported by those regarding the MF-dependence of the electrokinetic properties of nuclei in human buccal epithelial cells. 84 Other studies of general character were devoted to the analysis of the socalled "electromagnetic man" vs. the magnetic resonance tomography 85 and of the effect of MFs on synchronization of mammalian cell permeabilization vs. the MF-dependence of gene delivery. 86 From a methodological point of view, attempts were made to employ programmmed chronopotentiometry as a tool to study electroporation and resealing of pores in bilayer lipid membranes, 87 while an EU-wide initiative to characterize the biological effects of EMF on human and mouse cell lines by gene expression profiling was taken.…”

Section: Discussionmentioning

confidence: 99%

Interactions of zero-frequency and oscillating magnetic fields with biostructures and biosystems

Volpe

2003

Photochem Photobiol Sci

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This review points to the investigations concerning the effects of zero-frequency (DC) and oscillating (AC) magnetic fields (MFs) on living matter, and especially those exerted by weak DC and low-frequency/low-intensity AC MFs. Starting from the analysis of observations on the action of natural magnetic storms (MSs) or periodic geomagnetic field (GMF) variations on bacteria, plants and animals, which led to an increasing interest in MFs in general, this survey pays particular attention to the background knowledge regarding the action of artificial MFs not only at the ionic, molecular or macromolecular levels, but also at the levels of subcellular regions, in vitro cycling cells, in situ functioning tissues or organs and total bodies or entire populations. The significance of some crucial findings concerning, for instance, the MF-dependence of the nuclear or cellular volumes, rate of cell proliferation vs. that of cell death, extent of necrosis vs. that of apoptosis and cell membrane fluidity, is judged by comparing the results obtained in a solenoid (SLD), where an MF can be added to a GMF, with those obtained in a magnetically shielded room (MSR), where the MFs can be partially attenuated or null. This comparative criterion is required because the differences detected in the behaviour of the experimental samples against that of the controls are rather small per se and also because the evaluation of the data often depends upon the peculiarity of the methodologies used. Therefore, only very small differences are observed in estimating the MF-dependence of the expression of a single gene or of the rates of total DNA replication, RNA transcription and protein translation. The review considers the MF-dependence of the interactions between host eukaryotic cells and infecting bacteria, while documentation of the harmful effects of the MFs on specific life processes is reported; cases of favourable action of the MFs on a number of biological functions are also evidenced. In the framework of studies on the origin and adaptation of life on Earth or in the Universe, theoretical insights paving the way to elucidate the mechanisms of the MF interactions with biostructures and biosystems are considered.

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Section: Discussionmentioning

confidence: 99%

Interactions of zero-frequency and oscillating magnetic fields with biostructures and biosystems

Volpe

2003

Photochem Photobiol Sci

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“…This indicated that age-related differences in ENN between donors are connected with changes of cell nucleus charges, but not with cell membrane permeabilities [10,11]. Catabolic hormones, such as epinephrine, norepinephrine, hydrocortisone caused a significant ENN decrease [12].…”

Section: Introductionmentioning

confidence: 99%

Application of intracellular microelectrophoresis to analysis of the influence of the low-level microwave radiation on electrokinetic properties of nuclei in human epithelial cells

Shckorbatov¹,

Shakhbazov²,

Navrotskaya³

et al. 2002

Electrophoresis

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Intracellular microelectrophoresis was applied to investigate the electrokinetic properties of human buccal epithelium cell nuclei after exposure of cells to microwaves of wavelengths of 8 mm (f = 37.5 GHz) and 16 mm (f = 18.75 GHz) at a surface power density of 0.2 mW/cm(2). Irradiated or nonirradiated cells were suspended in a flat microelectrophoretic chamber and exposed to an electric field of 15 V/cm at a current flow of 0.1 mA. The cells, whose nuclei altered their intracellular location towards the anode of the externally applied electric field, were considered to have negatively charged nuclei. The percentage of cells with electrophoretically movable nuclei was determined as the value of electronegativity of cell nuclei (ENN). Microwaves induced changes of ENN during irradiation of 15-60 s. If cells of a donor had an elevated initial level of ENN, it decreased during irradiation. On the contrary, if cells of another donor had a low initial ENN level, irradiation induced ENN increase. No significant difference between the action of microwaves of wavelengths of 8 mm and 16 mm was found. However, microwave irradiation caused an increase in membrane permeability for the in vivo dye indigo Carmine in cells of all donors irrespectively of the initial levels they showed. This suggests that electrokinetic properties of nuclei in cells do not only depend on cell membrane permeability.

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Electrophoretic mobility of cell nuclei (EMN index) as a biomarker of the biological aging process: Considering the association between EMN index and age

Czapla

McPhail

2015

HOMO

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Modification of electrokinetic properties of nuclei in human buccal epithelial cells by electric fields

Cited by 3 publications

References 23 publications

Interactions of zero-frequency and oscillating magnetic fields with biostructures and biosystems

Interactions of zero-frequency and oscillating magnetic fields with biostructures and biosystems

Application of intracellular microelectrophoresis to analysis of the influence of the low-level microwave radiation on electrokinetic properties of nuclei in human epithelial cells

Electrophoretic mobility of cell nuclei (EMN index) as a biomarker of the biological aging process: Considering the association between EMN index and age

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