Microwave Sterilization of Plant Tissue Culture Media

Tisserat, Brent; Jones, Danny; Galletta, Paul D.

doi:10.21273/hortsci.27.4.358

Cited by 14 publications

(7 citation statements)

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“…Due to the advantages of micropropagation and to the need for reducing the costs involved, some alternatives to thermal sterilization have been examined. Latimer and Matsen (1977) suggested microwave sterilization, although they observed this method was inefficient in the sterilization of liquids due to medium overflow with elevation of temperature (Tisserat et al, 1992;Teixeira et al, 2005b). Another alternative is the sterilization of culture media by filtering, but it has proven to be inadequate when used for large volumes because it increases consumables and labor costs, and is timeconsuming (Tisserat et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Pais¹,

Silva²,

Souza³

et al. 2016

Afr. J. Biotechnol.

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Micropropagation requires controlling contamination that might compromise the success of the process. Thermal sterilization is traditionally used; however, costs deriving from equipment acquisition and maintenance render this technique costly. With the purpose of finding an alternative to thermal sterilization, this research aimed at assessing the efficiency and ideal concentration of sodium hypochlorite for sterilization of culture media and glassware used during rooting of micropropagated Gerbera hybrida cv. Essandre. Two experiments were carried out. In the first one, treatments consisted of control I (no sterilization), control II (thermal sterilization), and total active chlorine concentrations of 0.0005, 0.001, 0.002 and 0.003%. In the second experiment, based on the results observed in the first experiment, treatments consisted of control I (thermal sterilization) and II (chemical sterilization), and total active chlorine concentrations of 0.002, 0.0025 and 0.003%. Plant behavior was assessed based on the length of aerial part and roots, number of roots, and dry biomass of plants. Results showed that the addition of an active chlorine concentration of 0.003% to culture media provided total control of contaminants, and there were no significant differences regarding the variables analyzed between plants obtained with thermal sterilization and with sodium hypochlorite sterilization. Thus, chemical sterilization can be used as a replacement for thermal sterilization of nutrition media for rooting of gerbera in vitro.

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Section: Introductionmentioning

confidence: 99%

Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Pais¹,

Silva²,

Souza³

et al. 2016

Afr. J. Biotechnol.

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“…Teixeira and Torres (1998) suggested using a microwave oven, although Tisserat et al (1992), and later Teixeira et al (2005b), were unsuccessful in their trials. In theory, chemical sterilization is another possible but as yet little explored alternative.…”

Section: Introductionmentioning

confidence: 99%

Influence of NaClO on nutrient medium sterilization and on pineapple (Ananas comosus cv Smooth cayenne) behavior

Teixeira

Ribeiro

Teixeira

2006

Plant Cell Tiss Organ Cult

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A new protocol for the preparation and sterilization of media with sodium hypochlorite was tested for in vitro culturing of pineapple shoots. Total active chlorine concentrations equal to or higher than 0.0003%, as well as other asepsis procedures, provided complete sterilization of the medium. At a total active chlorine concentration of 0.0003%, pineapple shoots more than doubled both their biomass and the number of new shoots.

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“…Autoclaving of medium significantly raises the cost and extends the operating time of its preparation [Macek et al 1995]. Sterilization of plant cultures medium with high temperature and increased pressure causes the degradation of temperature labile components, changes the chemical composition and creates toxic products of degradation of sugars, such as furfural [Brondani et al 2013] The medium microwaving occurred unsuccessfully and is not often used for media preparation [Teixeira and Torres 1998;Tisserat et al 1992]. Chemical sterilization of medium may be an alternative to autoclaving.…”

Section: Discussionmentioning

confidence: 99%

MICROPROPAGATION OF STRAWBERRY (Fragaria × ananassa Duch.) ON CHEMICALLY STERILIZED MEDIA

Siekierzyńska¹,

Litwińczuk²

2018

Acta Sci.Pol.Hortorum Cultus

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Micropropagation of strawberry (Fragaria × ananassa Duch.) is a widely used technique to produce plantlets in commercial horticulture. Unfortunately, in contrast to propagation based on runners, it is also an expensive method and may lead to obtaining plants with changed phenotype. In order to reduce such problems, the influence of chemical method of medium sterilization (application of commercial home bleach-ACE ®) instead of autoclaving on the development of in vitro cultures of four strawberry cultivars ('Elsanta', 'Elkat', 'Senga Sengana', 'Polka') was examined. The presented study proved that sodium hypochlorite added into the hot medium is very effective, making aseptic environment agent. Applied in tested dose (2 cm 3 of ACE ® per dm-3) is not harmful to strawberry in vitro cultures and usually causes positive changes in their development (reduced formation of adventitious shoots and callus), except for 'Elsanta' cultivar. Thus the application of media sterilized with sodium hypochlorite is beneficial and cost effective and may be recommended both for commercial and especially amateur strawberry micropropagation.

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Microwave Sterilization of Plant Tissue Culture Media

Cited by 14 publications

References 0 publications

Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Influence of NaClO on nutrient medium sterilization and on pineapple (Ananas comosus cv Smooth cayenne) behavior

MICROPROPAGATION OF STRAWBERRY (Fragaria × ananassa Duch.) ON CHEMICALLY STERILIZED MEDIA

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