Midgut adaptation and digestive enzyme distribution in a phloem feeding insect, the pea aphid Acyrthosiphon pisum

Cristofoletti, Plı́nio T.; Ribeiro, Alberto F.; Deraison, Céline; Rahbé, Yvan; Terra, Walter R.

doi:10.1016/s0022-1910(02)00222-6

Cited by 206 publications

(175 citation statements)

References 31 publications

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“…It has also been demonstrated that they participate in other functions, such as immunological processes and tissue remodeling during insect metamorphosis (Matsumoto et al 1995;Tryselius & Hultmark 1997;Hashmi et al 2002;Baum et al 2007;Wang et al 2009a). The inhibition of cathepsin L protein activity by a protease inhibitor decreases the development and reproduction rate in Myzus persicae (Sulzer) (Aphidomorpha: Aphididae) (Cristofoletti et al 2003). We found that the cathepsin L gene of M. alternatus was down-regulated.…”

Section: Discussionmentioning

confidence: 74%

Bacteria Associated with the Guts of Two Wood-Boring Beetles: Anoplophora glabripennis and Saperda vestita (Cerambycidae)

et al. 2006

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Section: Discussionmentioning

confidence: 74%

Bacteria Associated with the Guts of Two Wood-Boring Beetles: Anoplophora glabripennis and Saperda vestita (Cerambycidae)

et al. 2006

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“…Other contigs with putative functional importance for aphid host-plant interaction, including chemosensory proteins (Table 1), are related to sensing and tasting of plant compounds (Wanner et al, 2005), which have been (Cristofoletti et al, 2003;Rahbé et al, 2003;Deraison et al, 2004).…”

Section: Xenobiotic Detoxificationmentioning

confidence: 99%

“…Cysteine proteinase inhibitors present in artificial diets or transgenic plants were shown to reduce the activity of cathepsins of M. persicae and consequently to reduce its performance (Rahbé et al, 2003). A contig related to juvenile hormone-binding proteins (Table 1), involved in resistance to juvenoid insecticides was also detected (Shemshedini & Wilson, 1990).…”

Section: Xenobiotic Detoxificationmentioning

confidence: 99%

Annotated expressed sequence tags and xenobiotic detoxification in the aphid Myzus persicae (Sulzer)

et al. 2007

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Aphids (Hemiptera: Aphididae) are phytophagous insects that are important agricultural pests. The enormous negative economic impacts caused by aphids worldwide are well known, and are mostly due to their high multiplication rate and the transmission of phytopathogenic viruses. Aphid management strategies mainly involve chemical treatments which are pollutants and are increasingly inefficient, since aphids have developed multiple insecticide-resistant mechanisms. Among the most economically important species is the green peach aphid Myzus persicae Sulzer (Aphididae: Macrosiphini), which is able to colonize a wide range of host plants belonging to many different families, and transmits numerous plant viruses. Because of its large prevalence, M. persicae has been the target of massive insecticide treatments; consequently, it has evolved several insecticide-resistant mechanisms. In this work, a collection of expressed genes from M. persicae is presented in order to identify putative genes involved in xenobiotic detoxification. After cDNA cloning and sequencing, 959 expressed sequence tags (EST) were annotated. Most sequences matched known genes corresponded to metabolism proteins (26%), ribosomal proteins (23%) and structural proteins (8%). Among them, several sequences corresponded to proteins putatively involved in sensing, degradation or detoxification of plant xenobiotic products.

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“…Finally, more complex situations can be envisaged. For example, CABYV and BWYV uptake could be mediated by a single receptor or receptor complex which is present in both the midgut and hindgut, but differences in the lumen "environment," such as pH, which is known to vary along the alimentary tract in aphids (11), could alter the affinity of the receptor (24) and/or the conformation of BWYV RTD so that it no longer binds to the hindgut-borne receptor without having such an effect on the CABYV RTD. Evidently, much remains to be learned about the specific tissue tropism of luteovirids and about luteovirid-vector interactions in general, and recombinant viruses, such as those described here, should be extremely useful in future investigations.…”

Section: Discussionmentioning

confidence: 99%

The Polerovirus Minor Capsid Protein Determines Vector Specificity and Intestinal Tropism in the Aphid

Brault¹,

Périgon²,

Reinbold³

et al. 2005

J Virol

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Aphid transmission of poleroviruses is highly specific, but the viral determinants governing this specificity are unknown. We used a gene exchange strategy between two poleroviruses with different vectors, Beet western yellows virus (BWYV) and Cucurbit aphid-borne yellows virus (CABYV), to analyze the role of the major and minor capsid proteins in vector specificity. Virus recombinants obtained by exchanging the sequence of the readthrough domain (RTD) between the two viruses replicated in plant protoplasts and in whole plants. The hybrid readthrough protein of chimeric viruses was incorporated into virions. Aphid transmission experiments using infected plants or purified virions revealed that vector specificity is driven by the nature of the RTD. BWYV and CABYV have specific intestinal sites in the vectors for endocytosis: the midgut for BWYV and both midgut and hindgut for CABYV. Localization of hybrid virions in aphids by transmission electron microscopy revealed that gut tropism is also determined by the viral origin of the RTD.The genera Polerovirus, Luteovirus, and Enamovirus constitute the Luteoviridae family (luteovirids). Luteovirids are restricted to the phloem tissue of host plants and are strictly transmitted by aphids in a persistent, circulative, and nonpropagative manner (17,20). Virions, acquired by aphids while feeding from sieve tubes of an infected plant, are transported through the gut epithelium and released in the hemolymph. In this compartment, virions interact with symbionin (of endosymbiont origin), which may protect them from the immune system and/or modify structural properties of virions (42). Virus particles in the hemolymph can be taken up by accessory salivary gland (ASG) epithelial cells from which they are released via the salivary duct during feeding.Transmission electron microscope (TEM) observations indicate that transport of virions through the gut and ASG epithelia occurs by receptor-mediated endocytosis/exocytosis. Passage across the gut wall can take place at two sites, the posterior midgut and the hindgut. Barley yellow dwarf virus-MAV (Luteovirus) (16), Cereal yellow dwarf virus (Polerovirus) (17), and Soybean dwarf virus (unassigned member of the Luteoviridae) (19) are internalized at the hindgut, while the posterior midgut is used by two poleroviruses, Beet western yellows virus (BWYV) (37) and Potato leafroll virus (14). Cucurbit aphid-borne yellows virus (CABYV) (Polerovirus) is unique among studied luteovirids in that its virions are taken up at both sites (38).Luteovirus transmission is highly specific (21), but the molecular mechanisms controlling specificity are unknown. One hypothesis is that specificity is mediated by interaction between motifs on the virion and receptors at the epithelial cell plasmalemma during endocytosis. Experiments with different combinations of luteovirids and vector or nonvector aphid species indicate that the basal plasmalemma of ASG epithelial cells is an important site for such differential interactions, although the ASG basal lamina...

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Midgut adaptation and digestive enzyme distribution in a phloem feeding insect, the pea aphid Acyrthosiphon pisum

Cited by 206 publications

References 31 publications

Bacteria Associated with the Guts of Two Wood-Boring Beetles: <I>Anoplophora glabripennis</I> and <I>Saperda vestita</I> (Cerambycidae)

Bacteria Associated with the Guts of Two Wood-Boring Beetles: <I>Anoplophora glabripennis</I> and <I>Saperda vestita</I> (Cerambycidae)

Annotated expressed sequence tags and xenobiotic detoxification in the aphid Myzus persicae (Sulzer)

The Polerovirus Minor Capsid Protein Determines Vector Specificity and Intestinal Tropism in the Aphid

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