Migration and Proliferative Activity of Mesenchymal Stem Cells in 3D Polylactide Scaffolds Depends on Cell Seeding Technique and Collagen Modification

Abstract

We analyzed viability of mesenchymal stem cells seeded by static and dynamic methods to highly porous fibrous 3D poly-L-lactide scaffolds with similar physical and chemical properties, but different spatial organization modified with collagen. Standard collagen coating promoted protein adsorption on the scaffold surface and improved adhesive properties of 100 μ-thick scaffolds. Modification of 600-μ scaffolds with collagen under pressure increased proliferative activity of mesenchymal stem cells seeded under static and dynamic (delivery of 100,000 cells in 10 ml medium in a perfusion system at a rate of 1 ml/min) conditions by 47 and 648%, respectively (measured after 120-h culturing by MTT test). Dynamic conditions provide more uniform distribution of collagen on scaffold fibers and promote cell penetration into 3D poly-L-lactide scaffolds with thickness >600 μ.

A New Bone Substitute Developed from 3D-Prints of Polylactide (PLA) Loaded with Collagen I: An In Vitro Study

“…These effects might be masked in our approach by collagen coating. Rodina tested migration and proliferation of murine mesenchymal stem cells on electrospun PLA scaffolds coated with collagen [ 41 , 42 ]—completely different to our scaffolds, but their study demonstrates possible combination of PLA and collagen. Concerning biocompatibility of PLA, our study is in accordance with the described literature, however, it is the first study analyzing 3D-printed PLA discs coated with collagen with different human cells in vitro.…”

Enhanced Wound Healing Potential of Primary Human Oral Fibroblasts and Periodontal Ligament Cells Cultured on Four Different Porcine-Derived Collagen Matrices

“…Chen et al have observed intermediate degrees of spreading and proliferation of vascular smooth muscle cells on an engineered model ECM consisting of fibrillary type I collagen and fibronectin compared to the cellular responses measured on collagen or fibronectin alone [ 57 ]. By investigating the migratory and proliferative activity of mesenchymal stem cells on 3D polylactide scaffolds, Rodina et al have concluded that larger pore size or higher porosity of the scaffold enhances the cellular penetration and growth [ 58 , 59 ]. Chemical, micron- and/or nano-scale topographical analyses of the matrices have not been performed in the present study and deserve detailed investigation.…”

The electrospinning of a thermo-responsive polymer with peptide conjugates for phenotype support and extracellular matrix production of therapeutically relevant mammalian cells

“…In general terms, these data were in accord with the improved results in 3D fibrous scaffolds for cell culture and tissue engineering compared to 2D cultures reported in recent years. [39][40][41][42][43] The fibrous structure in these materials was designed to mimic ECM properties, resulting in an increase in desired cell response. However, as the synthetic polymers used in our culture assays were non-native to the cells, we anticipated that peptide or protein incorporation or functionalisation would be required to overcome some key practical challenges.…”

“…These included poor cell attachment and induction of inflammation or undesired cell differentiation due to the suboptimal mechanical or chemical properties (such as stiffness and hydrophobicity) of conventional polymer fibres. [39][40][41][42][43] For example, Rodina et al 42 investigated migration and proliferation of mesenchymal stromal stem cells (MSSCs) on collagen protein modified PLA fibrous scaffolds. They observed more uniform distribution and cell penetration into these modified scaffolds.…”