Migration behavior of dyestuff-containing liposomes in capillary electrophoresis with chemiluminescence detection

Tsukagoshi, Kazuhiko; Okumura, Yukihisa; Nakajima, Riichiro

doi:10.1016/s0021-9673(98)00376-8

Cited by 41 publications

(36 citation statements)

References 15 publications

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“…The volume of the analyte plug moving in the separation channel was by far small enough compared to the volume of the reagent flowing in the CL reagent channel; therefore, the optimum solvent environment for CL reaction can be obtained by maintaining nonaqueous conditions on the point of detection, for this CL system was mainly dependent on the nonaqueous environment [33]. Under above conditions, dansyl-phenylalanine and -sarcosine were separated and detected on this microchip.…”

Section: Electrophoretic Separation Of Dansyl-phenylalanine and -Sarcmentioning

confidence: 99%

“…The second is the configuration of the CL detector, that is, the way for mixing CL reagent and the analytes. The peroxyoxalate CL system has been widely applicable to detection of fluorescence and fluorescence-labeled compounds in CE [18,32,33]. TCPO was representative as a peroxyoxalate CL reagent here.…”

Section: Analytical Performancementioning

confidence: 99%

“…So the concentration of hydrogen peroxide was set at <150 mM, which gave enough CL intensity with a large signal to noise ratio, and the mixture was prepared just before use to prevent decomposition of TCPO. This mode has been successfully realized on capillary electrophoresis [33,38]. Consequently, the acetonitrile experiments, the CL reagent flow rate of 15.0 L min −1 was taken.…”

Section: Mixing Mode Of the Analytes And The Chemiluminescent Reagentmentioning

confidence: 99%

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Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip

Lin

Uchiyama

et al. 2004

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Section: Electrophoretic Separation Of Dansyl-phenylalanine and -Sarcmentioning

confidence: 99%

Section: Analytical Performancementioning

confidence: 99%

Section: Mixing Mode Of the Analytes And The Chemiluminescent Reagentmentioning

confidence: 99%

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Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip

Lin

Uchiyama

et al. 2004

Talanta

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“…A CE-CL detector similar to that reported in the previous paper 8 was used for the present study. A fresh fused-silica capillary tube (50 µm i.d., 50 cm length) was treated with 1 M sodium hydroxide for 30 min and then washed with distilled water.…”

Section: Methodsmentioning

confidence: 99%

Application of Capillary Electrophoresis with Sensitive Detection to Analysis for Saccharide Molecules

et al. 2002

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Saccharide analyses have been one of the most important research themes in the fields of analytical chemistry, biochemistry and medicine.In general, absorption spectrophotometry is not directly applied to the detection of saccharide molecules, so that saccharide must be labeled with an absorption and fluorescence (FL) reagent for colorimetry and fluorometry. The chemiluminescence (CL) system using peroxyoxalate reagent, such as bis(2,4,6-trichlorophenyl)oxalate (TCPO), has been used together with dyestuffs as labeling reagents for trace analyses. It was reported that the sensitivity of the CL system was more than 10 times higher than that of a conventional FL system. 1,2 However, there are few reports with regard to application of the CL system to analysis of saccharide. 3,4 Capillary electrophoresis (CE) has been shown to be a powerful and efficient analytical separation technique. CL is expected to also be an ideal detection method for CE, due to its simple optical system and low background. Recently, the applicability of CL detection in CE has been successfully demonstrated. The CE-CL detector has been applied to various biological constituents, such as amino acids, peptides, and proteins. 5,6 We have developed 7 a CE-CL detector using luminol, peroxyoxalate (TCPO), and ruthenium(II) complex reagent. Here, we applied for the first time our CE-CL detector using TCPO and dyestuff to analysis of saccharide. ExperimentalAll reagents used were commercially available or special grade. Ion-exchanged water was distilled for use. D-Maltose, Dglucose, D-mannose, D-xylose, D-erythlose, and TCPO were purchased from Wako Pure Chemical Industries, Ltd. 8-Aminonaphthalene-1,3,6-trisulfonic acid (ANTS), 4-amino-5-hydroxynaphthalene-2,7-disulfonic acid (AHNS), and 5-amino-2-naphthalenesulfonic acid (ANA) were purchased from Molecular Probes.Acetic acid (14 µl) and 1 M sodium cyanotrihydroborate (50 µl) were added to 0.1 M ANTS aqueous solution (185 µl). To each solution was added one of several concentrations of saccharide aqueous solution (250 µl). The mixture solution was heated at 90˚C for 60 min, and then diluted with a migration buffer solution as needed.A CE-CL detector similar to that reported in the previous paper 8 was used for the present study. A fresh fused-silica capillary tube (50 µm i.d., 50 cm length) was treated with 1 M sodium hydroxide for 30 min and then washed with distilled water. A migration buffer solution (10 mM carbonate; pH 9.0) was treated by a filter and degassed for use. The capillary tube was filled with the buffer solution in advance. The sample solution (ca. 10 nl) was directly introduced into a capillary tube of positive electrode side for 10 s from 30 cm height by siphoning. A high voltage of 10 kV was applied to the electrodes using a DC power supplier (Model HCZE-30PNO. 25, Matsusada Precision Devices Co., LTD.). The detection cell was a 500 µm i.d. Teflon tube, which was covered with black Tygon-tape and had a 8 mm detection length in front of a photomultiplier (Hamamatsu Photonics Co., L...

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“…[6][7][8] A high voltage (12 kV) was applied to electrodes using a DC power supply (Model HCZE-30PNO.25, Matsusada Precision Devices Co. Ltd.). A fusedsilica capillary of 50 cm length and 75 µm i.d.…”

Section: Methodsmentioning

confidence: 99%

Capillary Electrophoresis with Chemiluminescent Detection for Luminol Using Potassium Ferricyanide as a Catalyst

2001

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Absorption and fluorescence detectors have been most commonly used for capillary electrophoresis (CE), which is performed with on-capillary detection. On the other hand, several groups have studied CE with a chemiluminescence (CL) detector. [1][2][3][4] This system can offer excellent analytical selectivity and sensitivity. 5 We have applied several CL reagents to CE with a CL detector, such as luminol, 6 peroxyoxalate, 3,7 and Ru(II) complex. 8 Luminol and isoluminol isothiocyanatelabeled compounds were analyzed using CE with a CL detector and microperoxidase as a catalyst. 6 However, microperoxidase is expensive, and sometimes has problems in a capillary due to its large molecular size.In this study, we examined the electrophoretic behavior of ferricyanide ion in a capillary by use of a batch-type detection cell. On the basis of the obtained information, we proposed and developed CE with a CL detector using potassium ferricyanide, instead of microperoxidase, as a catalyst for determining luminol or its derivative. A repeated injection analysis of luminol was achieved by using a flow-type CL detection cell. ExperimentalAll of the reagents used were of commercially available special grade. Ion-exchanged water was distilled for use. Luminol, potassium ferricyanide, and H2O2 were purchased from Nacalai Tesque, Inc.An apparatus of CE with a CL detector was made while referring to previous work. 6-8 A high voltage (12 kV) was applied to electrodes using a DC power supply (Model HCZE-30PNO.25, Matsusada Precision Devices Co. Ltd.). A fusedsilica capillary of 50 cm length and 75 µm i.d. (GL Sciences Inc.) was used. Luminol was used as a model sample. The sample was dissolved in a 10 mM phosphate buffer (pH 10.8), which was used as a migration buffer. Sample injections were performed by siphoning from a height of 20 cm for 10 s. The sample of luminol was migrated toward the CL detection cell, batch-or flow-type, and then mixed with the reagent. The resulting CL at the capillary outlet was captured by a photomultiplier tube (PM) equipped in a CL detector (Model EN-21, Kimoto Electric, Inc.).The concept of a batch-type CL detection cell was originally proposed by us in a previous paper. 6 The detection cell was made of Teflon, which had a 4 cm outer diameter, 2.5 cm height, and an 8 ml inner volume. An optical fiber (core diameter of 2 mm), a capillary, and a platinum wire as a grounding electrode were fixed to the cell. That is, the detection cell also served as an outlet reservoir. The optical fiber was set up straight to the capillary with a distance of 0.3 mm between them. When the sample emerged from the capillary, it reacted with the reagent at the capillary outlet to produce visible light. In the batch-type cell, potassium ferricyanide (25 mM) as a catalyst and H2O2 (10 mM) were added to the inlet and outlet reservoirs, respectively. Usage A: the catalyst was added to the inlet reservoir and H2O2 was added to the outlet reservoir or the CL detection cell, and in reverse Usage B: H2O2 was added to the inlet reservo...

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Migration behavior of dyestuff-containing liposomes in capillary electrophoresis with chemiluminescence detection

Cited by 41 publications

References 15 publications

Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip

Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip

Application of Capillary Electrophoresis with Sensitive Detection to Analysis for Saccharide Molecules

Capillary Electrophoresis with Chemiluminescent Detection for Luminol Using Potassium Ferricyanide as a Catalyst

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