Migration of bovine aortic smooth muscle cells after wounding injury. The role of hyaluronan and RHAMM.

Savani, Rashmin C.; Wang, Chao; Yang, Baichun; Zhang, Shiwen; Kinsella, Michael G.; Wight, Thomas N.; Stern, Robert; Nance, Dwight M.; Turley, Eva A.

doi:10.1172/jci117764

Cited by 191 publications

(168 citation statements)

References 44 publications

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“…The doublet bands are not specific because they are detected in RHAMM Ϫ/Ϫ cell extracts, as well as in cell extracts lacking RHAMM because of small interfering RNA treatment (results not shown). The same 70-kDa RHAMM protein was detected after scratch wounding in vitro and excisional wounding in vivo (41). The level of the 70-kDa RHAMM protein expression detected in splenocyte extracts of arthritic CD44-deficient, WT, and heterozygous mice was similar (Fig.…”

Section: Rhamm Compensates For Cd44 In Arthritic Cd44-deficient Micesupporting

confidence: 55%

RHAMM, a receptor for hyaluronan-mediated motility, compensates for CD44 in inflamed CD44-knockout mice: A different interpretation of redundancy

Nedvetzki

Gonen

Assayag

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

182

175

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We report here that joint inflammation in collagen-induced arthritis is more aggravated in CD44-knockout mice than in WT mice, and we provide evidence for molecular redundancy as a causal factor. Furthermore, we show that under the inflammatory cascade, RHAMM (receptor for hyaluronan-mediated motility), a hyaluronan receptor distinct from CD44, compensates for the loss of CD44 in binding hyaluronic acid, supporting cell migration, up-regulating genes involved with inflammation (as assessed by microarrays containing 13,000 cDNA clones), and exacerbating collagen-induced arthritis. Interestingly, we further found that the compensation for loss of the CD44 gene does not occur because of enhanced expression of the redundant gene (RHAMM), but rather because the loss of CD44 allows increased accumulation of the hyaluronic acid substrate, with which both CD44 and RHAMM engage, thus enabling augmented signaling through RHAMM. This model enlightens several aspects of molecular redundancy, which is widely discussed in many scientific circles, but the processes are still ill defined

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Section: Rhamm Compensates For Cd44 In Arthritic Cd44-deficient Micesupporting

confidence: 55%

RHAMM, a receptor for hyaluronan-mediated motility, compensates for CD44 in inflamed CD44-knockout mice: A different interpretation of redundancy

Nedvetzki

Gonen

Assayag

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

182

175

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“…Hyaluronan and versican have been found to play a role in the maintenance of proliferative and migratory phenotypes in various cells following growth factor treatment or injury [15,[56][57][58][59][60][61][62][63]. Hyaluronan is localized in tissues and cell cultures using a highly specific biotinylated hyaluronan binding protein (bHABP) preparation from cartilage [64].…”

Section: Role For the Pericellular Matrix In Cell Proliferation And Mmentioning

confidence: 99%

Hyaluronan-dependent pericellular matrix☆

Evanko¹,

Tammi²,

Tammi³

et al. 2007

Advanced Drug Delivery Reviews

260

238

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Hyaluronan is a multifunctional glycosaminoglycan that forms the structural basis of the pericellular matrix. Hyaluronan is extruded directly through the plasma membrane by one of three hyaluronan synthases and anchored to the cell surface by the synthase or cell surface receptors such as CD44 or RHAMM. Aggregating proteoglycans and other hyaluronan-binding proteins, contribute to the material and biological properties of the matrix and regulate cell and tissue function. The pericellular matrix plays multiple complex roles in cell adhesion/de-adhesion, and cell shape changes associated with proliferation and locomotion. Time-lapse studies show that pericellular matrix formation facilitates cell detachment and mitotic cell rounding. Hyaluronan crosslinking occurs through various proteins, such as tenascin, TSG-6, inter-alpha-trypsin inhibitor, pentraxin and TSP-1. This creates higher order levels of structured hyaluronan that may regulate inflammation and other biological processes. Microvillous or filopodial membrane protrusions are created by active hyaluronan synthesis, and form the scaffold of hyaluronan coats in certain cells. The importance of the pericellular matrix in cellular mechanotransduction and the response to mechanical strain are also discussed.

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“…Although originally considered an inert filling material HA is now known to have a number of biological activities including wound healing [1], angiogenesis [2][3] and cancer cell metastasis [4]. In many cases, HA biological function is thought to be related to its molecular size.…”

Section: Introductionmentioning

confidence: 99%

Development of a fluorescent substrate to measure hyaluronidase activity

Zhang

Mummert

2008

Analytical Biochemistry

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A novel fluorescent substrate (termed FRET-HA) to quantitatively assess hyaluronidase activity was developed. Hyaluronan (HA), the major substrate for hyaluronidase, was dual labeled with fluorescein amine and rhodamine B amine. The fluorescein amine fluorescence signal was significantly quenched while the rhodamine B amine signal was significantly enhanced due to fluorescence resonance energy transfer (FRET). In the presence of bovine testes hyaluronidase, cleavage of HA disrupted FRET resulting in a loss of the fluorescein amine quenching that was dependent upon both enzyme concentration and time. Increase in the fluorescein amine signal could be conveniently monitored in both non-continuous and continuous fashions. The K m value for bovine testes hyaluronidase was determined using FRET-HA in a continuous fluorescent assay. Importantly, the estimated K m value for bovine testes hyaluronidase using FRET-HA as the substrate was in excellent agreement with K m values previously reported for this enzyme using native (i.e., unlabeled) HA. Therefore, FRET-HA is a reliable substrate for quantitatively assessing the HA/ hyaluronidase molecular interaction. The simplicity, sensitivity and versatility of the FRET-HA substrate suggest that it will have utility in a variety of assay platforms and should be a new tool for assessing hyaluronidase activity.

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Migration of bovine aortic smooth muscle cells after wounding injury. The role of hyaluronan and RHAMM.

Cited by 191 publications

References 44 publications

RHAMM, a receptor for hyaluronan-mediated motility, compensates for CD44 in inflamed CD44-knockout mice: A different interpretation of redundancy

RHAMM, a receptor for hyaluronan-mediated motility, compensates for CD44 in inflamed CD44-knockout mice: A different interpretation of redundancy

Hyaluronan-dependent pericellular matrix☆

Development of a fluorescent substrate to measure hyaluronidase activity

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