Migration of Mesenchymal Stem Cells of Bursal Tissue after Rotator Cuff Repair in Rats

Safi, Elem; Ficklscherer, Andreas; Bondarava, Maryna; Betz, Oliver B.; Zhang, Anja; Jansson, Volkmar; Müller, Peter E.

doi:10.1055/s-0038-1636948

Cited by 11 publications

(4 citation statements)

References 29 publications

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“…In studies regarding rotator cuff repair after injury, some researchers have suggested that healing always seems to begin on the lateral side of the bursa. 12,22,26,30 Intriguingly, histologic cartilage staining reportedly shows that the generation of new cartilage in the tendon-bone transition area is always faster on the lateral side of the tendon. 31,32 Thus, the lateral side is the main and more reliable source of rotator cuff healing, and the healing speed is significantly faster than that of the medial side, especially at the tendon-bone junction.…”

Section: Discussionmentioning

confidence: 99%

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Microfracture Lateral to the Greater Tuberosity of the Humerus Enhances Tendon-to-Bone Healing in a Rat Rotator Cuff Model

et al. 2023

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Background: Microfracture at the rotator cuff insertion is an established surgical marrow-stimulation technique for enhancing rotator cuff healing. However, the effect of lateralized or medialized microfracture on the insertion is unknown. Purpose: To compare the biomechanical and histologic effects of microfracture at 3 different regions for rotator cuff repair in a rat model. Study Design: Controlled laboratory study. Methods: A total of 72 Sprague-Dawley rats with bilateral supraspinatus tendon insertion detachment were allocated into 4 groups with 4 different interventions: no microfracture at the humeral head as a control group (Con), traditional microfracture at the footprint area (MFA), and medialized microfracture to the footprint area (MMFA) on the articular surface of the humerus or lateralized microfracture to the footprint area at the greater tuberosity (LMFA). All underwent immediate repair. Tendon-to-bone healing was assessed by biomechanical and histologic tests 4 and 8 weeks postoperation. Results: At 4 weeks, the LMFA group showed a significantly superior failure load compared with the other groups (all P < .05). The LMFA and MFA groups showed significantly superior stiffness compared with the Con and MMFA groups (all P < .01). At 8 weeks, superior failure load and stiffness were observed in the LMFA group compared with the control group (all P < .05). Histologic examination revealed that the LMFA group had superior collagen composition and tendon-to-bone maturation at the interface at 4 and 8 weeks compared with the Con group (all P < .05). Conclusion: Lateralized microfracture at the greater tuberosity improved the histologic quality of repair tissue and biomechanical strength at the tendon-to-bone insertion after rotator cuff repair in a rat model. Clinical Relevance: Microfracture lateral to the footprint area might be a better way to enhance rotator cuff healing clinically.

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Section: Discussionmentioning

confidence: 99%

“…Several recent studies have shown that the lateral tendon is promising and important in healing promotion in the shoulder joint. 12,18,22,26 However, no relevant study has explored lateralized microfracture in an animal model.…”

mentioning

confidence: 99%

Microfracture Lateral to the Greater Tuberosity of the Humerus Enhances Tendon-to-Bone Healing in a Rat Rotator Cuff Model

et al. 2023

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“…46,47 The subacromial bursa is a good candidate source of MSCs in rotator cuff tears. 48,49 In addition, MSCs were found in bursal tissue infiltrate in repaired rotator cuff tendons after rotator cuff repair in an animal model. 50 Rotator cuff MSCs also express lineage-specific genes, such as Runt-related transcription factor 2 ( Runx2 ) and osteocalcin in osteogenic induction, peroxisome proliferator-activated receptor gamma ( PPARγ ) and lipoprotein lipase ( LPL ) in adipogenic differentiation, and aggrecan and collagen type II α1 ( ColIIα1 ) in chondrogenic differentiation.…”

Section: Discussionmentioning

confidence: 99%

Effects of a graphene oxide-alginate sheet scaffold on rotator cuff tendon healing in a rat model

Yoon

Kim

Min

et al. 2022

J Orthop Surg (Hong Kong)

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Background Natural polymer scaffolds used to promote rotator cuff healing have limitations in terms of their mechanical and biochemical properties. This animal study aimed to investigate the effects of combined graphene oxide (GO) and alginate scaffold and the toxicity of GO on rotator cuff healing in a rat model. Methods First, the mechanical properties of a GO/alginate scaffold and a pure alginate scaffold were compared. The in vitro cytotoxicity of and proliferation of human tenocytes with the GO/alginate scaffold were evaluated by CCK-8 assay. For the in vivo experiment, 20 male rats were randomly divided into two groups ( n = 10 each), and supraspinatus repair was performed: group 1 underwent supraspinatus repair alone, and group 2 underwent supraspinatus repair with the GO/alginate scaffold. Biomechanical and histological analyses were performed to evaluate the quality of tendon-to-bone healing 8 weeks after rotator cuff repair. Results The GO/alginate scaffold exhibited an increased maximum load ( p = .001) and tensile strength ( p = .001). In the cytotoxicity test, the cell survival rate with the GO/alginate scaffold was 102.08%. The proliferation rate of human tenocytes was no significant difference between the GO/alginate and alginate groups for 1, 3, 5, and 7 days. Biomechanically, group 2 exhibited a significantly greater ultimate failure load ( p < .001), ultimate stress ( p < .001), and stiffness ( p < .001) than group 1. The histological analysis revealed that the tendon-to-bone interface in group 2 showed more collagen fibers bridging, tendon-to-bone integration, longitudinally oriented collagen fibers, and fibrocartilage formation than in group 1. Conclusion A small amount of GO added to alginate improved the mechanical properties of the scaffold without evidence of cytotoxicity. At 8 weeks after rotator cuff repair, the GO/alginate scaffold improved tendon-to-bone healing without causing any signs of toxicity in a rat model.

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“…None of the small animal studies included in this review specifically addressed MSC homing to tendon lesions. However, when bursal tissue was implanted in rotator cuff tendon lesions in a rat model, the green fluorescent protein-labeled mesenchymal stem cells from this tissue infiltrated the healing tendons [159], demonstrating the presence of homing signals. Accordingly, ASC infiltration into the tendons was also evident when cell sheets were used as delivery vehicle in a canine model [126].…”

Section: In Vivo Evidencementioning

confidence: 99%

Mechanisms of Action of Multipotent Mesenchymal Stromal Cells in Tendon Disease

Burk¹

2019

Tendons

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Multipotent mesenchymal stromal cells (MSCs) are a promising therapeutic tool to treat tendon disease. Aiming to establish successful treatment approaches and to fully exploit the regenerative potential of the MSC, it is crucial to understand their mechanisms of action. However, these can be multifaceted and strongly context-sensitive and are still not well-understood in the context of tendon disease. This review aims to shed light on the different possible mechanisms, including engraftment, tenogenic differentiation, extracellular matrix synthesis and remodeling, immunomodulation, pro-angiogenetic effects, trophic support, and protection of resident tendon cells. Evidence from experimental and clinical (veterinary) case studies was compiled and interpreted in conjunction with the respective in vitro and animal models used.

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Migration of Mesenchymal Stem Cells of Bursal Tissue after Rotator Cuff Repair in Rats

Cited by 11 publications

References 29 publications

Microfracture Lateral to the Greater Tuberosity of the Humerus Enhances Tendon-to-Bone Healing in a Rat Rotator Cuff Model

Microfracture Lateral to the Greater Tuberosity of the Humerus Enhances Tendon-to-Bone Healing in a Rat Rotator Cuff Model

Effects of a graphene oxide-alginate sheet scaffold on rotator cuff tendon healing in a rat model

Mechanisms of Action of Multipotent Mesenchymal Stromal Cells in Tendon Disease

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