Migration speed of nucleolus precursor bodies in human male pronuclei: a novel parameter for predicting live birth

Inoue, Taketo; Taguchi, Sayumi; Uemura, Mikiko; Tsujimoto, Yoshiko; Miyazaki, Kazunori; Yamashita, Yoshiki

doi:10.1007/s10815-021-02172-7

Cited by 11 publications

(28 citation statements)

References 48 publications

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“…The warmed blastocysts were annotated according to the published consensus definitions and guidelines [ 28 , 29 ]. Beginning at the time of blastocyst re-expansion, the cross-sectional area (in µm 2 ) was recorded using the Embryoscope viewer’s elliptical measurement tool by circumscribing the outer periphery of the trophectoderm excluding the zona pellucida.…”

Section: Methodsmentioning

confidence: 99%

Clinical Usability of Embryo Development Using a Combined Qualitative and Quantitative Approach in a Single Vitrified-Warmed Blastocyst Transfer: Assessment of Pre-Vitrified Blastocyst Diameter and Post-Warmed Blastocyst Re-Expansion Speed

Park

Ahn

Seok

et al. 2022

JCM

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Improving the safety and efficacy of assisted reproductive technology programs has been a continuous challenge. Traditionally, morphological grading has been used for embryo selection. However, only a few studies have assessed the morphokinetic variables and morphological dynamics of blastocysts. In the present study, we aimed to perform a quantitative analysis of blastocyst diameter and re-expansion speed. This in-depth morphokinetic evaluation can correlate with currently observed pregnancy outcomes. In total, 658 single vitrified-warmed blastocyst transfer cycles were performed between October 2017 and December 2021, which were divided into four groups according to the pre-vitrified blastocyst diameter. After warming, the groups were subdivided according to the blastocyst re-expansion speed. These quantitative measurements were performed using a time-lapse system. Both diameter and speed are essential in determining the blastocyst quality, while age, day of freezing, and blastocyst quality are crucial from a clinical perspective. The application of both quantitative (diameter and speed) and qualitative (blastocyst quality scores) parameters can help evaluate the clinical usability of blastocysts. This method can prove useful for embryologists in counseling their patients and determining pregnancy patient-oriented strategies.

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Section: Methodsmentioning

confidence: 99%

Clinical Usability of Embryo Development Using a Combined Qualitative and Quantitative Approach in a Single Vitrified-Warmed Blastocyst Transfer: Assessment of Pre-Vitrified Blastocyst Diameter and Post-Warmed Blastocyst Re-Expansion Speed

Park

Ahn

Seok

et al. 2022

JCM

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“…Nevertheless, in bovine zygotes, as in other species, it seems that appropriate organization of chromosomes is essential for healthy embryo development [ 87 ]. In human embryos, the nucleoli in PNs are visible, they can be relatively easily tracked and closely mirror the organization of pronuclear chromatin [ 87 , 88 , 89 ]. On the other hand, in bovine zygotes, the nucleoli are not visible under a light microscope [ 18 ].…”

Section: The Possible Function Of the Nuclear Components And Their Po...mentioning

confidence: 99%

“…On the other hand, in bovine zygotes, the nucleoli are not visible under a light microscope [ 18 ]. This prevents the use of nucleolar dynamics in zygotes of species such as bovine or sheep as potentially attractive noninvasive indicators of development, as has been suggested for human embryos [ 88 , 89 ].…”

Section: The Possible Function Of the Nuclear Components And Their Po...mentioning

confidence: 99%

Nucleus reprogramming/remodeling through selective enucleation (SE) of immature oocytes and zygotes: a nucleolus point of view

Fulka

Loi

Palazzese

et al. 2022

Journal of Reproduction and Development

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It is now approximately 25 years since the sheep Dolly, the first cloned mammal where the somatic cell nucleus from an adult donor was used for transfer, was born. So far, somatic cell nucleus transfer, where G1-phase nuclei are transferred into cytoplasts obtained by enucleation of mature metaphase II (MII) oocytes followed by the activation of the reconstructed cells, is the most efficient approach to reprogram/remodel the differentiated nucleus. In general, in an enucleated oocyte (cytoplast), the nuclear envelope (NE, membrane) of an injected somatic cell nucleus breaks down and chromosomes condense. This condensation phase is followed, after subsequent activation, by chromatin decondensation and formation of a pseudo-pronucleus (i) whose morphology should resemble the natural postfertilization pronuclei (PNs). Thus, the volume of the transferred nuclei increases considerably by incorporating the content released from the germinal vesicles (GVs). In parallel, the transferred nucleus genes must be reset and function similarly as the relevant genes in normal embryo reprogramming. This, among others, covers the relevant epigenetic modifications and the appropriate organization of chromatin in pseudo-pronuclei. While reprogramming in SCNT is often discussed, the remodeling of transferred nuclei is much less studied, particularly in the context of the developmental potential of SCNT embryos. It is now evident that correct reprogramming mirrors appropriate remodeling. At the same time, it is widely accepted that the process of rebuilding the nucleus following SCNT is instrumental to the overall success of this procedure. Thus, in our contribution, we will mostly focus on the remodeling of transferred nuclei. In particular, we discuss the oocyte organelles that are essential for the development of SCNT embryos.

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“… 15 Whether these differences in the fertilization process affect NPB migration speed or the ability of NPB migration speed to predict LB was unclear after our previous study. 11 Therefore, in this study, we investigated the relationship between NPB migration speed, ploidy status, and clinical outcomes after assisted reproductive technology (ART) in IVF‐derived zygotes to determine if differences in the fertilization procedure affect NPB migration and the clarify the relationship between NPB migration, ploidy, and LB in IVF‐derived zygotes.…”

Section: Introductionmentioning

confidence: 99%

The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth

Inoue

Taguchi²,

Uemura

et al. 2023

Reprod Medicine & Biology

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Purpose To study the relationship between clinical outcomes after assisted reproduction and the migration speed of nucleolus precursor bodies (NPBs) in male and female pronuclei (mPN; fPN). Methods NPB migration speed, embryo ploidy status, and live birth (LB) were retrospectively analyzed in IVF‐derived zygotes. The central coordinates of the mPN, fPN, and NPBs were noted at multiple timepoints. The migration distance of NPBs between two sequential images was measured to calculate NPB migration speed. Results The NPB migration speeds in mPN and fPN were significantly faster in euploid zygotes than in aneuploid zygotes. In multivariate logistic analysis, NPB migration speed in mPN and the female age were associated with euploidy. The NPB migration speeds in mPN and fPN were also significantly faster in zygotes that led to LB than in zygotes that led to no pregnancy. In a receiver operating characteristic curve analysis of LB by NPB migration speed in mPN, the cut‐off value was 3.74 μm/h (AUC: 0.825, 95%CI: 0.688–0.963). When the zygotes were categorized by this cut‐off value, there were significantly more LBs in zygotes with migration speed ≥ the cut‐off (78.9% vs. 21.1%). Conclusions Zygotes with quickly migrating NPBs demonstrated the developmental potential to become a baby.

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Migration speed of nucleolus precursor bodies in human male pronuclei: a novel parameter for predicting live birth

Cited by 11 publications

References 48 publications

Clinical Usability of Embryo Development Using a Combined Qualitative and Quantitative Approach in a Single Vitrified-Warmed Blastocyst Transfer: Assessment of Pre-Vitrified Blastocyst Diameter and Post-Warmed Blastocyst Re-Expansion Speed

Clinical Usability of Embryo Development Using a Combined Qualitative and Quantitative Approach in a Single Vitrified-Warmed Blastocyst Transfer: Assessment of Pre-Vitrified Blastocyst Diameter and Post-Warmed Blastocyst Re-Expansion Speed

Nucleus reprogramming/remodeling through selective enucleation (SE) of immature oocytes and zygotes: a nucleolus point of view

The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth

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