Mikropropagasi Ramin (Gonytilus bancanus(Miq) Kurz) dari Eksplan Batang satu buku secara in Vitro

Yelnititis, Yelnititis; Komar, T.E.

doi:10.20886/jpth.2011.5.3.149-157

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“…Sampai sekarang, laporan keberhasilan perbanyakan ramin melalui kultur jaringan masih sangat terbatas. Yelnititis & Komar, ( 2011) melaporkan bahwa tunas ramin yang diinduksi dari eksplan batang satu buku dengan menggunakan zat pengatur tumbuh (ZPT) Benzyl adenine (BA) masih mengalami pertumbuhan yang lambat. Sebagai alternatif lain dilakukan penelitian menggunakan embriogenesis somatik.…”

Section: Introductionunclassified

Embentukan Embrio Somatik Dari Eksplan Daun Ramin, Spesies Tanaman Langka

Yelnititis

2021

JPTH

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Ramin (Gonystylus bancanus (Miq.) Kurz) is one of the most valuable timbers in Indonesia and the most over-exploited woody species, on the other hand,the success in propagation techniques are still limited. This species has been listed in Appendix II of CITES since 2004as the number of trees and populations continuouslydecline. Tissue culture has been explored for mass propagation, however, this technique still facesthechallenge, mainly in shoot elongation regenerated from single node explants. The purpose of these experiments is to selectthe best auxin (2,4-D, picloram,dan dicamba) and the best concentration of Benzyl Adenine(BA) treatment on somaticembryo formation. A series of experiments of somatic embryogenesis from leaf explant for ramin were conducted at Tissue Culture Laboratory, Center for Forest Biotechnology and Tree Improvement Yogyakarta. Modified Murashige and Skoog (MS) media supplemented with 0,1 mg/Lthiamine; 0,5 mg/l nicotinic acid; 0,5 mg/Lpyridoxine; 2.0 mg/Lglycineand 100 mg/Lmyo-inositolwere used as growth medium. In thisstudy, three different auxins were used as treatments: 2,4-D(2,4 dichlorophenoxy acetic acid), picloram,and dicamba appliedat6.0 mg/L. The observation was made on the texture of callus formed and the performance of the somatic embryos obtained. The results showed thatthe texture of callus obtained is compact formed callus and green in color. The best treatment to induce globular somatic embryos is using 6.0 mg/Lpicloram within eight months. Thebest treatment to induce globular and torpedo somatic embryos is BA 3.0 mg/L treatment

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Section: Introductionunclassified

Embentukan Embrio Somatik Dari Eksplan Daun Ramin, Spesies Tanaman Langka

Yelnititis

2021

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The prospect of micropropagating Gonystylus bancanus (Miq.) Kurz, a tropical peat swamp forest timber species through tissue culture technique - Review

Akeng¹,

Aini²,

Akhirrudin³

et al. 2018

J. For. Sci.

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Grippin A., Nor Aini A.S., Nor Akhirrudin M., Hazandy A.H., Sures Kumar M., Ismail P. (2018): The prospect of micropropagating Gonystylus bancanus (Miq.) Kurz, a tropical peat swamp forest timber species through tissue culture technique -Review. J. For. Sci., 64: 1-8.Gonystylus bancanus (Miquel) Kurz is one of the most valuable timber species in tropical peat swamp forest. Its timber is widely used for furniture, decorative cabinets and interior decoration. Due to its higher demand, G. bancanus continues to be harvested which leads to its disappearance in the wild. This is worsened by the fact that germination rates in the wild are very poor due to rapid insect and fungal infestation. Even though vegetative propagation via stem cuttings has been successful, proper protocols on tissue culture need to be explored as a viable way of mass production of a large number of planting stock in a relatively shorter time for forest rehabilitation and enrichment planting purposes.

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Mikropropagasi Ramin (Gonytilus bancanus(Miq) Kurz) dari Eksplan Batang satu buku secara in Vitro

Abstract: Tabel 1. Waktu induksi tunas dan penampakan visual biakan dari beberapa konsentrasi thidiazuron, BA dan kinetin

Cited by 2 publications

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Embentukan Embrio Somatik Dari Eksplan Daun Ramin, Spesies Tanaman Langka

Embentukan Embrio Somatik Dari Eksplan Daun Ramin, Spesies Tanaman Langka

The prospect of micropropagating Gonystylus bancanus (Miq.) Kurz, a tropical peat swamp forest timber species through tissue culture technique - Review

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