Mild Hyperthermia Promotes and Accelerates Development and Maturation of Erythroid Cells

Tounkara, Fatoumata Korika; Dumont, Nellie; Fournier, S; Boyer, Lucie; Nadeau, Paul; Pineault, Nicolas

doi:10.1089/scd.2012.0112

Cited by 3 publications

(4 citation statements)

References 39 publications

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“…Mammalian cells are usually cultured at 37°C in the incubator supplied with 5% of CO 2 unless specific research purpose is required [7]. Previous studies showed that low temperature decreases cell growth rate and affects embryo development [8–10]; whereas mild heat stress enhances cell proliferation rate and accelerates development [11–12]. In addition, the viability for mammalian cells or embryos can be severely affected after long-term treatment at sub-zero temperature [13, 14].…”

Section: Introductionmentioning

confidence: 99%

The analysis of viability for mammalian cells treated at different temperatures and its application in cell shipment

et al. 2017

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Mammalian cells are very important experimental materials and widely used in biological and medical research fields. It is often required that mammalian cells are transported from one laboratory to another to meet with various researches. Conventional methods for cell shipment are laborious and costive despite of maintaining high viability. In this study we aimed to develop a simple and low-cost method for cell shipment by investigating the viabilities of different cell lines treated at different temperatures. We show that the viability of mammalian cells incubated at 1°C or 5°C significantly reduced when compared with that at 16°C or 22°C. Colony formation assays revealed that preservation of mammalian cells at 1°C or 5°C led to a poorer recovery than that at 16°C or 22°C. The data from proliferation and apoptotic assays confirmed that M2 cells could continue to proliferate at 16°C or 22°C, but massive death was caused by apoptosis at 1°C or 5°C. The morphology of mammalian cells treated under hypothermia showed little difference from that of the untreated cells. Quantitative RT-PCR and alkaline phosphatase staining confirmed that hypothermic treatment did not change the identity of mouse embryonic stem cells. A case study showed that mammalian cells directly suspended in culture medium were able to be shipped for long distance and maintained a high level of viability and recovery. Our findings not only broaden the understanding to the effect of hypothermia on the viability of mammalian cells, but also provide an alternative approach for cell shipment.

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Section: Introductionmentioning

confidence: 99%

The analysis of viability for mammalian cells treated at different temperatures and its application in cell shipment

et al. 2017

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“…According to previous studies, low temperature can decrease cell growth rate (Azambuja et al, 1998; Kaushik & Kaur, 2005; Vergara et al, 2014), although modest heat stress increases cell proliferation and growth (Choudhery et al, 2015; Tounkara et al, 2012). Increasingly, long‐term treatment at subzero temperatures can influence the viability rate of mammalian cells (Jin et al, 2008; Moussa et al, 2008; Wang et al, 2017).…”

Section: Discussionmentioning

confidence: 93%

Section: Sucrose-based Mediamentioning

confidence: 89%

Recent developments in cell shipping methods

Heydarzadeh

Kia

Boroomand

et al. 2022

Biotech & Bioengineering

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As opposed to remarkable advances in the cell therapy industry, research reveal inexplicable difficulties associated with preserving and post‐thawing cell death. Post cryopreservation apoptosis is a common occurrence that has attracted the attention of scientists to use apoptosis inhibitors. Transporting cells without compromising their survival and function is crucial for any experimental cell‐based therapy. Preservation of cells allows the safe transportation of cells between distances and improves quality control testing in clinical and research applications. The vitality of transported cells is used to evaluate the efficacy of transportation strategies. For many decades, the conventional global methods of cell transfer were not only expensive but also challenging and had adverse effects. The first determination of some projects is optimizing cell survival after cryopreservation. The new generation of cryopreservation science wishes to find appropriate and alternative methods for cell transportation to ship viable cells at an ambient temperature without dry ice or in media‐filled flasks. The diversity of cell therapies demands new cell shipping methodologies and cryoprotectants. In this review, we tried to summarize novel improved cryopreservation methods and alternatives to cryopreservation with safe and viable cell shipping at ambient temperature, including dry preservation, hypothermic preservation, gel‐based methods, encapsulation methods, fibrin microbeads, and osmolyte solution compositions.

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“…Notably, several studies have demonstrated that increased reactive oxygen species may reduce the stemness of hematopoietic stem cells [ 19 , 20 ] and promote lineage commitment [ 21 ]. In a recent study, it was demonstrated that intracellular reactive oxygen species might be involved in promoting erythroid lineage differentiation of human cord blood CD34+ cells under mild hyperthermia stress condition [ 22 ]. In light of these findings, we propose that the observed erythroid expansion induced by ATO may be mediated by a redox-dependent mechanism that enhances differentiation to the erythroid lineage.…”

Section: Discussionmentioning

confidence: 99%

Arsenic Primes Human Bone Marrow CD34+ Cells for Erythroid Differentiation

Zhang

Wang

Chen

et al. 2015

Bioinorganic Chemistry and Applications

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Arsenic trioxide exhibits therapeutic effects on certain blood malignancies, at least partly by modulating cell differentiation. Previous in vitro studies in human hematopoietic progenitor cells have suggested that arsenic may inhibit erythroid differentiation. However, these effects were all observed in the presence of arsenic compounds, while the concomitant cytostatic and cytotoxic actions of arsenic might mask a prodifferentiating activity. To eliminate the potential impacts of the cytostatic and cytotoxic actions of arsenic, we adopted a novel protocol by pretreating human bone marrow CD34+ cells with a low, noncytotoxic concentration of arsenic trioxide, followed by assaying the colony forming activities in the absence of the arsenic compound. Bone marrow specimens were obtained from chronic myeloid leukemia patients who achieved complete cytogenetic remission. CD34+ cells were isolated by magnetic-activated cell sorting. We discovered that arsenic trioxide enhanced the erythroid colony forming activity, which was accompanied by a decrease in the granulomonocytic differentiation function. Moreover, in erythroleukemic K562 cells, we showed that arsenic trioxide inhibited erythrocyte maturation, suggesting that arsenic might have biphasic effects on erythropoiesis. In conclusion, our data provided the first evidence showing that arsenic trioxide could prime human hematopoietic progenitor cells for enhanced erythroid differentiation.

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Mild Hyperthermia Promotes and Accelerates Development and Maturation of Erythroid Cells

Cited by 3 publications

References 39 publications

The analysis of viability for mammalian cells treated at different temperatures and its application in cell shipment

The analysis of viability for mammalian cells treated at different temperatures and its application in cell shipment

Recent developments in cell shipping methods

Arsenic Primes Human Bone Marrow CD34+ Cells for Erythroid Differentiation

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