Mild hypothermia improves brain injury in rats with intracerebral hemorrhage by inhibiting IRAK2/NF‐κB signaling pathway

Abstract

Objective To explore the effect of mild hypothermia on nerve injury by establishing a rat model of intracerebral hemorrhage (ICH), and to clarify the specific molecular mechanism of mild hypothermia in improving brain injury in ICH rats. Methods The rat model of ICH was established by collagenase injection. The neurological deficit score (NDS), brain tissue water detection, and Nissl staining were applied to detect the degree of brain injury. ELISA was used to analyze the expression of proinflammatory cytokines and serum nerve injury indexes. Flow cytometry and Western Blot were used to detect neuronal apoptosis. Results Mild hypothermia treatment significantly improved the brain injury of the ICH rats and down‐regulated the inflammatory response and oxidative stress in the brain tissue. Moreover, mild hypothermia also effectively inhibited IRAK2/NF‐κB signaling pathway and thus affect neuronal apoptosis. Conclusion Mild hypothermia alleviates inflammatory response and neuronal apoptosis by inhibiting IRAK2/NF‐κB signaling pathway in the ICH rats thus improving brain injury.

Interleukin-1 receptor-associated kinase 2 promotes inflammatory reactions by activating the nuclear factor kappa-B signaling pathway in diabetic nephropathy

“…IRAK2 is essential in the toll-like receptor and IL-1R signaling pathways, ultimately activating the NF-κB pathway [ 23 ]. The IRAK2/NF-κB signaling pathway plays roles in various diseases, such as brain injury [ 24 ]. We further explored the relationship between IRAK2 and the NF-κB signaling pathway in DN.…”

“…IRAK2 regulates various signaling pathways, including the nuclear factor-kappa B (NF-κB) signaling pathway [ 11 ]. IRAK2 can lead to TRAF6 ubiquitination, which is a key event in NF-κB activation [ 12 ]. The NF-κB signaling pathway is a critical cellular signaling pathway that regulates a wide range of biological processes, such as immune responses, inflammation, cell proliferation, and survival [ 13 ].…”

Effect of Angong Niuhuang pill combined with mild therapeutic hypothermia on acute cerebral hemorrhage in patients

“…Previous research by Metz suggested that a temperature of 32.5 -35 o C is conducive to reducing intracranial pressure [16]. In recent years, mild therapeutic hypothermia (with a temperature of 30 -35 °C) has achieved favorable outcomes in treating cerebral ischemia, cerebral hypoxia, and cerebral hemorrhage [17,18]. The results of the current study indicated that Angong Niuhuang Pil/mild therapeutic hypothermia significantly reduced the levels of NSE, S100B, and MBP proteins and mitigated the severity of neuron damage in the bleeding site.…”

Mild hypothermia alleviates oxygen-glucose deprivation/reperfusion-induced apoptosis by inhibiting ROS generation, improving mitochondrial dysfunction and regulating DNA damage repair pathway in PC12

“…At present, mild hypothermia has been used in lots of neurologic emergencies with considerable success, such as ischemic stroke, subarachnoid hemorrhage, traumatic brain injury, hepatic encephalopathy, cardiopulmonary arrest and so on [6]. It is reported that this may play its therapeutic role in a number of ways, such as regulated lysosomal function, autophagic ux, caspase-3 activation and the Wnt/b-catenin signaling pathway in our previous study [7][8][9], there are also calcium in ux, miR-122, IGF-1R/AKT pathway, IRAK2/NF-κB signaling pathway, etc [10][11][12]. In addition, DNA damage is de ned as physical or chemical changes in DNA within cells that affect the interpretation and transmission of genetic information [13].…”