Mild Hypothermia Protects Pigs’ Gastric Mucosa After Cardiopulmonary Resuscitation via Inhibiting Interleukin 6 (IL-6) Production

Abstract

BackgroundThe purpose of this study was to determine the effect of mild hypothermia therapy on gastric mucosa after cardiopulmonary resuscitation (CPR) and the underlying mechanism.Material/MethodsVentricular fibrillation was induced in pigs. After CPR, the surviving pigs were divided into mild hypothermia-treated and control groups. The changes in vital signs and hemodynamic parameters were monitored before cardiac arrest and at intervals of 0.5, 1, 2, 4, 6, 12, and 24 h after restoration of spontaneous circulation. Serum IL-6 was determined at the same time, and gastroscopy was performed. The pathologic changes were noted, and the expression of IL-6 was determined by hematoxylin and eosin (HE) staining and immunohistochemistry under light.ResultsThe heart rate, mean arterial blood pressure, and cardiac output in both groups did not differ significantly. The gastric mucosa ulcer index evaluated by gastroscopy 2 h and 24 h after restoration of spontaneous circulation (ROSC) in the mild hypothermic group was lower than that the control group (P<0.05). The inflammatory pathologic score of gastric mucosa in the mild hypothermic group 6–24 h after ROSC was lower than that in the control group (P<0.05). Serum and gastric mucosa IL-6 expression 0.5–4 h and 6, 12, and 24 h after ROSC was lower in the mild hypothermic group than in the control group (P<0.05).ConclusionsMild hypothermia treatment protects gastric mucosa after ROSC via inhibiting IL-6 production and relieving the inflammatory reaction.

Mild Therapeutic Hypothermia Protects from Acute and Chronic Renal Ischemia-Reperfusion Injury in Mice by Mitigated Mitochondrial Dysfunction and Modulation of Local and Systemic Inflammation

“…In the renal tissue, IR also increased IL-6 mRNA, and mTH was associated with less IL-6 mRNA at 2 h, 24 h, and 1 month after reperfusion. mTH has already been associated with a decrease in IL-6 secretion in a model of cardiopulmonary resuscitation in pigs [ 36 ], in line with our results. In order to explain the mTH-mediated plasma IL-10 decrease, one could think that a significant limitation of the IR insult causes a diminished inflammatory response and therefore less IL-10 secretion.…”

Intervention effects of lotus leaf flavonoids on gastric mucosal lesions in mice infected with Helicobacter pylori

“…As a chemokine, IL-6 can make inammatory cells chemotactic, causing a large number of inammatory mediators to be produced and released, leading to destruction of the gastric mucosal barrier, aggravation of lesions, and gastric mucosal inammation. 36 IL-12 is also a cytokine closely related to inammation, which shows in abnormally high levels in the case of inammation of gastric tissues. 37 IFN-g is a cytokine produced by activated T cells and NK cells.…”