Milk miRNAs encapsulated in exosomes are stable to human digestion and permeable to intestinal barrier in vitro

Rani, Payal; Vashisht, Monika; Golla, Naresh; Shandilya, Shruti; Onteru, Suneel Kumar; Singh, Dheer

doi:10.1016/j.jff.2017.05.009

Cited by 91 publications

(115 citation statements)

References 45 publications

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“…This study reported milk microRNAs relative resistance to digestion, microRNA degradation kinetics, and it found their associations with more than one kind of digestion‐resistant EVs (Benmoussa et al., ). Afterwards, several studies confirmed these results in different in vitro digestion models and pushed forward the investigations by studying the internalization of digested milk microRNAs and EVs by recipient cells (Benmoussa et al., ; Kahn et al., ; Liao et al., ; Rani et al., ).…”

Section: Milk Micrornas In Health and Diseasementioning

confidence: 93%

“…Other recent reports suggested that these EVs and associated microRNAs can also be transduced through the digestive tract epithelium in vitro (Rani et al., ; Wolf et al., ).…”

Section: Milk Micrornas In Health and Diseasementioning

confidence: 97%

“…Milk EVs have been extensively studied for their biological functions upon consumption (Agrawal et al., ; Aqil et al., ; Aqil, Munagala, Jeyabalan, Agrawal, & Gupta, ; Arntz et al., ; Badawy, El‐Magd, & AlSadrah, ; Leiferman et al., ; Matsuda & Patel, ; Munagala, Aqil, Jeyabalan, & Gupta, ; Munagala et al., ; Oliveira et al., , 2017) and numerous studies have reported that milk EVs are bioaccessible (survive digestion; Benmoussa et al., ; Kahn et al., ; Liao et al., ; Rani et al., ; Shandilya, Rani, Onteru, & Singh, ; Vashisht, Rani, Onteru, & Singh, ) and bioavailable (Le Doare et al., ; Wang et al., ; Zempleni, ; Zempleni et al., ).…”

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

“…According to more than 30 studies, from different teams around the world, milk EVs resisted in vitro digestion (Benmoussa et al., ; Kahn et al., ; Liao et al., ; Rani et al., ; Shandilya et al., ; Vashisht et al., ) were internalized by recipient cells upon co‐incubation (Admyre et al., , 2008; Aqil et al., , ; Badawy et al., ; Golan‐Gerstl et al., ; Hock et al., ; Kahn et al., ; Liao et al., ; Maburutse, Park, Oh, & Kim, ; Martin et al., ; Matsuda & Patel, ; Mobley et al., ; Munagala et al., ; Naslund et al., ; Qin et al., ; Vashisht, Rani, Onteru, & Singh, ; Yu et al., ), transduced through the intestinal wall in vitro (Rani et al., ; Shandilya et al., ; Wolf et al., ), and were detected in animal models after ingestion (Manca et al., ; Munagala et al., ) or intravenous injection (Somiya, Yoshioka, & Ochiya, ).…”

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

“…Evidence for the bioavailability of milk EVs. Milk EVs have been extensively studied for their biological functions upon consumption Aqil et al, 2017a;Aqil, Munagala, Jeyabalan, Agrawal, & Gupta, 2017b;Arntz et al, 2015;Badawy, El-Magd, & AlSadrah, 2018;Leiferman et al, 2018;Matsuda & Patel, 2018;Oliveira et al, 2016Oliveira et al, , 2017 and numerous studies have reported that milk EVs are bioaccessible (survive digestion; Benmoussa et al, 2016;Kahn et al, 2018;Liao et al, 2017;Rani et al, 2017; and bioavailable (Le Doare et al, 2018;Wang et al, 2018;Zempleni, 2017;Zempleni et al, 2017).…”

Section: Milk Evs: Functional Carriers Of Micrornasmentioning

confidence: 99%

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Milk MicroRNAs in Health and Disease

Benmoussa

Provost

2019

Comp Rev Food Sci Food Safe

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MicroRNAs are small noncoding RNAs responsible for regulating 40% to 60% of gene expression at the posttranscriptional level. The discovery of circulating microRNAs in several biological fluids opened the path for their study as biomarkers and long‐range cell‐to‐cell communication mediators. Their transfer between individuals in the case of blood transfusion, for example, and their high enrichment in milk have sparked the interest for microRNA transfer through diet, especially from mothers to infants during breastfeeding. The extension of such paradigm led to the study of milk microRNAs in the case of cow or goat milk consumption in adults. Here we provide a comprehensive critical review of the key findings surrounding milk microRNAs in human, cow, and goat milk among other species. We discuss the data on their biological properties, their use as disease biomarkers, their transfer between individuals or species, and their putative or verified functions in health and disease of infants and adult consumers. This work is based on all the literature available and integrates all the results, theories, debates, and validation studies available so far on milk microRNAs and related areas of investigations. We critically discuss the limitations and outline future aspects and avenues to explore in this rapidly growing field of research that could impact public health through infant milk formulations or new therapies. We hope that this comprehensive review of the literature will provide insight for all teams investigating milk RNAs’ biological activities and help ensure the quality of future reports.

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Section: Milk Micrornas In Health and Diseasementioning

confidence: 93%

“…Other recent reports suggested that these EVs and associated microRNAs can also be transduced through the digestive tract epithelium in vitro (Rani et al., ; Wolf et al., ).…”

Section: Milk Micrornas In Health and Diseasementioning

confidence: 97%

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

Section: Milk Evs: Functional Carriers Of Micrornasmentioning

confidence: 99%

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Milk MicroRNAs in Health and Disease

Benmoussa

Provost

2019

Comp Rev Food Sci Food Safe

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Harnessing the Natural Healing Power of Colostrum: Bovine Milk‐Derived Extracellular Vesicles from Colostrum Facilitating the Transition from Inflammation to Tissue Regeneration for Accelerating Cutaneous Wound Healing

Kim

Han

et al. 2021

Adv Healthcare Materials

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As wound healing is an extremely complicated process, consisting of a cascade of interlocking biological events, successful wound healing requires a multifaceted approach to support appropriate and rapid transitions from the inflammatory to proliferative and remodeling phases. In this regard, here the potential use of bovine milk extracellular vesicles (EVs) to enhance wound healing is investigated. The results show that milk EVs promote fibroblast proliferation, migration, and endothelial tube formation. In particular, milk EVs derived from colostrum (Colos EVs) contain various anti‐inflammatory factors facilitating the transition from inflammation to proliferation phase, as well as factors for tissue remodeling and angiogenesis. In an excisional wound mouse model, Colos EVs promote re‐epithelialization, activate angiogenesis, and enhance extracellular matrix maturation. Interestingly, Colos EVs are further found to be quite resistant to freeze‐drying procedures, maintaining their original characteristics and efficacy for wound repair after lyophilization. These findings on the superior stability and excellent activity of milk Colos EVs indicate that they hold great promise to be developed as anti‐inflammatory therapeutics, especially for the treatment of cutaneous wounds.

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Guidelines for separation of natural extracellular vesicles

Liang,

Li,

et al. 2024

Food Safety and Health

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Extracellular vesicles (EVs) are found everywhere in different biofluids such as blood, saliva, urine, cerebrospinal fluid, and breast milk. They have sizes ranging from 20 to 400 nm and contain a wide range of cargo, including membrane proteins, periplasmic proteins, and nucleic acids. EVs possess the remarkable ability to diffuse, traverse the mucus layer, migrate directly to other tissues, and interact with various cells of the host immune system. Consequently, researchers have harnessed their potential as delivery carriers for novel immuno‐drugs or bioactive components. The separation of EVs from food can be considered a safe and effective method for harnessing their beneficial effects. In this guide, we present isolation protocols for natural EVs derived from plants, dairy products, cells, and microbes. This includes steps for raw material processing or culture as well as conditions for separation and purification. Moreover, these protocols can serve as a foundation for preparing and isolating engineered EVs. In summary, the utilization of natural EVs presents a vast array of opportunities in the fields of biotechnology, biomedicine, and food nutrition.

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Milk miRNAs encapsulated in exosomes are stable to human digestion and permeable to intestinal barrier in vitro

Cited by 91 publications

References 45 publications

Milk MicroRNAs in Health and Disease

Milk MicroRNAs in Health and Disease

Harnessing the Natural Healing Power of Colostrum: Bovine Milk‐Derived Extracellular Vesicles from Colostrum Facilitating the Transition from Inflammation to Tissue Regeneration for Accelerating Cutaneous Wound Healing

Guidelines for separation of natural extracellular vesicles

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