Milk odd- and branched-chain fatty acids as biomarkers of rumen function—An update

Fievez, Veerle; Colman, E.; Castro-Montoya, J.; Stefanov, Ivan; Vlaeminck, Bruno

doi:10.1016/j.anifeedsci.2011.12.008

Cited by 249 publications

(300 citation statements)

References 68 publications

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“…The fact that the concentration of 10-oxo-18:0, a metabolite resulting from a pathway involving the hydration and subsequent oxidation of dietary unsaturated FA, was lower in the sainfoin incubations would be in line with the lack of differences in the OBCFA, since all these FA have been suggested as indicators of altered rumen environment or microbial biomarkers (Jenkins et al, 2008;Fievez et al, 2012). Surprisingly, because it would be expected that the inhibitory effect of tannins on ruminal BH would lead to lower 18:0 accumulation in the digesta and numerical results seem to corroborate it, differences were only significant at 6 h of incubation.…”

Section: Ruminal Biohydrogenationmentioning

confidence: 85%

Effects of a tannin-rich legume (Onobrychis viciifolia) on in vitro ruminal biohydrogenation and fermentation

Toral

Hervás

Missaoui

et al. 2016

Span. j. agric. res.

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There is still controversy surrounding the ability of tannins to modulate the ruminal biohydrogenation (BH) of fatty acids (FA) and improve the lipid profile of milk or meat without conferring a negative response in the digestive utilization of the diet. Based on this, an in vitro trial using batch cultures of rumen microorganisms was performed to compare the effects of two legume hays with similar chemical composition but different tannin content, alfalfa and sainfoin (Onobrychis viciifolia), on the BH of dietary unsaturated FA and on the ruminal fermentation. The first incubation substrate, alfalfa, was practically free of tannins, while the second, sainfoin, contained 3.5% (expressed as tannic acid equivalents). Both hays were enriched with sunflower oil as a source of unsaturated FA. Most results of the lipid composition analysis (e.g., greater concentrations of 18:2n-6, cis-9 18:1 or total polyunsaturated FA in sainfoin incubations) showed the ability of this tannin-containing legume to inhibit the BH process. However, no significant differences were detected in the accumulation of cis-9 trans-11 conjugated linoleic acid, and variations in trans-11 18:1 and trans-11 cis-15 18:2 did not follow a regular pattern. Regarding the rumen fermentation, gas production, ammonia concentration and volatile FA production were lower in the incubations with sainfoin (‒17, ‒23 and ‒11%, respectively). Thus, although this legume was able to modify the ruminal BH, which might result in improvements in the meat or milk lipid profile, the present results were not as promising as expected or as obtained before with other nutritional strategies.

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Section: Ruminal Biohydrogenationmentioning

confidence: 85%

Effects of a tannin-rich legume (Onobrychis viciifolia) on in vitro ruminal biohydrogenation and fermentation

Toral

Hervás

Missaoui

et al. 2016

Span. j. agric. res.

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“…In this study, supplementation with SO may have influenced the FA synthase activity of ruminal microorganisms, specifically from Prevotella spp. and B. fibrisolvens (Fievez et al, 2012). It has been suggested that higher proportions of iso-FA in solidassociated bacteria reflect their enrichment in cellulolytic bacteria (e.g.…”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of ruminal bacterial populations involved in lipid metabolism in dairy cows fed different vegetable oils

Vargas‐Bello‐Pérez

Cancino‐Padilla

Romero

et al. 2016

Animal

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Vegetable oils are used to increase energy density of dairy cow diets, although they can provoke changes in rumen bacteria populations and have repercussions on the biohydrogenation process. The aim of this study was to evaluate the effect of two sources of dietary lipids: soybean oil (SO, an unsaturated source) and hydrogenated palm oil (HPO, a saturated source) on bacterial populations and the fatty acid profile of ruminal digesta. Three non-lactating Holstein cows fitted with ruminal cannulae were used in a 3 × 3 Latin square design with three periods consisting of 21 days. Dietary treatments consisted of a basal diet (Control, no fat supplement) and the basal diet supplemented with SO (2.7% of dry matter (DM)) or HPO (2.7% of DM). Ruminal digesta pH, NH 3 -N and volatile fatty acids were not affected by dietary treatments. Compared with control and HPO, total bacteria measured as copies of 16S ribosomal DNA/ml by quantitative PCR was decreased ( P < 0.05) by SO. Fibrobacter succinogenes, Butyrivibrio proteoclasticus and Anaerovibrio lipolytica loads were not affected by dietary treatments. In contrast, compared with control, load of Prevotella bryantii was increased ( P < 0.05) with HPO diet. Compared with control and SO, HPO decreased ( P < 0.05) C18:2 cis n-6 in ruminal digesta. Contents of C15:0 iso, C18:11 trans-11 and C18:2 cis-9, trans-11 were increased ( P < 0.05) in ruminal digesta by SO compared with control and HPO. In conclusion, supplementation of SO or HPO do not affect ruminal fermentation parameters, whereas HPO can increase load of ruminal P. bryantii. Also, results observed in our targeted bacteria may have depended on the saturation degree of dietary oils.

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“…BCFA largely derive from bacteria leaving the rumen (Fievez et al, 2012). Particularly, iso-BCFA are synthesized by cellulolytic strains (the microorganisms mainly involved in ruminal BH; Kepler and Tove, 1967), consequently being considered valuable predictors of ruminal bacterial activity.…”

Section: " "mentioning

confidence: 99%

Browsing ratio, species intake, and milk fatty acid composition of goats foraging on alpine open grassland and grazable forestland

Iussig

Renna

Gorlier

et al. 2015

Small Ruminant Research

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Aims of this study were to investigate diet selection of dairy goats foraging on two alpine vegetation types and to assess the related effects on milk fatty acid (FA) composition. Two enclosures laid out on an open grassland (OG) and a grazable forestland (GF) were exploited by 14 Camosciata goats. A commercial concentrate was supplemented during milking. Forty-five plots were randomly selected inside each enclosure and used to assess the species relative abundance (SRA) and phenological stage of plants, as well as goats' preferences (browsing ratio, BR) and intake (SI) for each species.Representative samples of the diet in each enclosure were built up considering the most ingested plant species and plant parts. Feed samples were analyzed for proximate, FA, and phenolic compositions.Milk samples were collected in each enclosure and analyzed for their FA profile. The enclosures showed a similar level of vegetation diversity. If compared to the GF enclosure, the OG one had higher proportion of Poaceae (81.2 vs 44.8% of SRA) and lower proportion of non-legume dicotyledons (14.6 vs 50.7% of SRA). The goats mostly selected eutrophic species in OG and forbs and woody species leaves in GF. The ingested vegetation was almost completely represented by grasses in OG (89.6% of SI), and by similar proportions of grasses and herbs and woody species in GF (54.9 and 45.1% of SI, respectively). The ingested forages from the OG and GF showed a comparable proximate composition; if compared to OG, the GF vegetation type was however richer in α-linolenic acid (ALA) and phenolic compounds (PC). Fatty acid analysis showed that GF milk had higher concentrations of ALA, total omega-3 FA, total trans-octadecadienoic acids, total conjugated linoleic acids, total trans-octadecenoic acids, and a reduced omega-6/omega-3 FA ratio than OG milk.Branched-chain FA were not significantly affected by treatment, suggesting that the activity of ruminal bacteria in the goats was not inhibited by the higher concentration of PC in GF plants. The main reason for the observed improvement of the FA profile in GF milk seems to be attributable to the FA profile rather than the concentration and composition of PC of the ingested plants. The abundance of herbs and woody species in the ingested vegetation was positively associated with the presence of nutritionally desirable FA in goat milk fat.

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Milk odd- and branched-chain fatty acids as biomarkers of rumen function—An update

Cited by 249 publications

References 68 publications

Effects of a tannin-rich legume (Onobrychis viciifolia) on in vitro ruminal biohydrogenation and fermentation

Effects of a tannin-rich legume (Onobrychis viciifolia) on in vitro ruminal biohydrogenation and fermentation

Quantitative analysis of ruminal bacterial populations involved in lipid metabolism in dairy cows fed different vegetable oils

Browsing ratio, species intake, and milk fatty acid composition of goats foraging on alpine open grassland and grazable forestland

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