Milk protein variants are highly associated with milk performance traits in East Friesian Dairy and Lacaune sheep

Giambra, Isabella Jasmin; Brandt, H.; Erhardt, G.

doi:10.1016/j.smallrumres.2014.09.001

Cited by 30 publications

(39 citation statements)

References 69 publications

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“…In contrast, Giambra et al . () report a significant effect of CSN1S1 genotype on milk protein content in East Friesian and Lacaune ewes. Technical (statistical methods and associated false positive rates) and biological (differential genetic and environmental effects and their interactions) factors could be the cause of the discrepancies about the effect of CSN1S1 on milk traits reported in the present and other studies.…”

Section: Discussionmentioning

confidence: 97%

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Variations at regulatory regions of the milk protein genes are associated with milk traits and coagulation properties in the Sarda sheep

et al. 2016

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Regulatory variation at the ovine casein genes could have important effects on the composition and coagulation properties of milk. Herewith, we have partially resequenced the promoters and the 3'-UTR of the four casein genes in 25 Sarda sheep. Alignment of these sequences allowed us to identify a total of 29 SNPs. This level of polymorphism (one SNP every 250 bp) is remarkably high if compared with SNP densities estimated in human genic regions (approximately one SNP per bp). The 29 SNPs identified in our resequencing experiment, plus three previously reported SNPs mapping to the lactalbumin, alpha (LALBA) and β-lactoglobulin (BLG, also known as progestagen-associated endometrial protein, PAEP) genes, were genotyped with a multiplex TaqMan Open Array Real-Time PCR assay in 760 Sarda sheep with records for milk composition and coagulation properties. Association analysis revealed the existence of significant associations of CSN1S2 and CSN3 genotypes with milk protein and casein contents. Moreover, genotypes at CSN1S1 were significantly associated with rennet coagulation time, curd firming time and curd firmness, whereas CSN2 was associated with curd firming time. These results suggest that SNPs mapping to the promoters and 3'-UTRs of ovine casein genes may exert regulatory effects on gene expression and that they could be used for improving sheep milk quality and technological traits at the population level through marker assisted selection.

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Section: Discussionmentioning

confidence: 97%

“…Interestingly, Giambra et al . () report a significant association between CSN3 isoelectric focusing variants, computed as single or interaction effects, on protein content in East Friesian dairy ewes. In goats, an analogous influence of CSN3 on fat and protein content has been evidenced by Hayes et al .…”

Section: Discussionmentioning

confidence: 98%

Variations at regulatory regions of the milk protein genes are associated with milk traits and coagulation properties in the Sarda sheep

et al. 2016

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“…This DNA-based test can be used for typing camel CSN1S1 variability independent of age, gender and lactation stage of the animals, which can be useful for broader analysis of camel milk protein variability. This is a requirement for further analyses concerning associations between milk protein variability and milk performance traits in camel, like already done for cattle, goat, and sheep (Caroli et al, 2009;Giambra et al, 2011;Martin et al, 2002). The information gained can be used to increase the economic value of camel milk production or to ameliorate the supply of the nomads, as well as producing camel diversified milk.…”

Section: Dna-based Testmentioning

confidence: 99%

“…As associations between milk protein variability and milk performance traits are reported in ruminants (Caroli, Chessa, & Erhardt, 2009;Giambra, Brandt, & Erhardt, 2011;Martin, Szymanowska, Zwierzchowski, & Leroux., 2002), further research is needed for camel milk proteins. In addition, milk protein variants are also used for breed characterization (Ceriotti, Marletta, Caroli, & Erhardt, 2004) and analyses of biodiversity (Ibeagha-Awemu, Jann, Weimann, .…”

Section: Introductionmentioning

confidence: 99%

Biochemical and molecular characterization of polymorphisms of αs1-casein in Sudanese camel (Camelus dromedarius) milk

Shuiep

Giambra

Zubeir

et al. 2013

International Dairy Journal

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“…Caravaca et al (2008) reported that in Murciano-Granadina goats, BB genotype (8.50±0.60 g/l) was significantly associated with increased levels of CSN1S1 (P<0.05), whereas BF (6.56±0.82 g/l), EE (6.39±0.60 g/l) and EF (6.91±0.76 g/l) genotypes displayed non-significant differences when compared with each other while in Malaguena breed, average values of CSN1S1 content estimated for BB, BF, EE and FF genotypes were 6.94±0.38, 5.36±0.22, 4.58±0.13 and 3.98±0.27 g/l, respectively, being all significantly different (P<0.05). Montalbano et al (2014) in Girgentana goats reported that A, B and F alleles were associated with a content of s1-casein in milk of 3.2 ± 0.4, 5.4 ± 0.5 and 0.7 ± 0.1 g/l, respectively, whereas N variant was a 'null' allele associated with the absence of s1-casein in milk.. Giambra et al (2014) reported CSN1S1* C' and C" alleles and showed that the CSN1S1*C" allele had association with a higher protein content in East Friesian Dairy and Lacaune (LAC) sheep breeds.…”

Section: Association Of Csn1s1 Gene Variants With Milk Production Andmentioning

confidence: 99%

Association of the CSN1S1 gene polymorphism with milk composition traits in Jamunapari goat

Singh

Rout

et al. 2018

IJAR

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The genetic polymorphism of the CSN1S1 (casein alpha-s 1 ) locus was investigated in Jamunapari goat breed by Sodium Dodicyle Sulphate -Poly Acrylamide Gel Electrophoresis (SDS-PAGE) and Polymerase Chain Reaction -Restriction Fragment Length Polymorphism (PCR-RFLP). A total of 122 milk and blood samples were collected. The SDS-PAGE pattern revealed the presence of four variants of CSN1S1 locus namely, A, B, E and F. The A allele of -s1 -casein was observed in the majority of goats. The genotypic frequency of the AA, BB, BE, AF and FF genotypes of CSN1S1 locus were 0.437, 0.175, 0.250, 0.125 and 0.012, respectively. The PCR-RFLP was performed, six different variant groups of F allele (224)bp, the B/E allelic pair (161+63) bp, A/0 allelic pair (150+63) bp, AD allelic pair (212+150+63) bp, AF allelic pair (224+150+63) bp and BF allelic pair (224+160+63) were obtained after restriction digestion with XmnI. The genotypic frequency of AA, AD, AF, BB, BE, BF and BC genotypes were 0.274, 0.114, 0.147, 0.098, 0.163, 0.081 and 0.163, respectively. The allelic frequency of A, B, C, D, E and F were 0.360, 0.303, 0.081, 0.057, 0.081 and 0.114, respectively.The present study showed that AA genotype had significant effect on total protein content on the milk and AD genotype had significant effect on 90 day milk yield in Jamunapari goat. Thus the CSN1S1 genotype information in Jamunapari could be utilised in selection strategies, in order to select genetic stock for the production of goat milk with higher protein content in marker assisted selection to increase the rate of genetic gain.

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Milk protein variants are highly associated with milk performance traits in East Friesian Dairy and Lacaune sheep

Cited by 30 publications

References 69 publications

Variations at regulatory regions of the milk protein genes are associated with milk traits and coagulation properties in the Sarda sheep

Variations at regulatory regions of the milk protein genes are associated with milk traits and coagulation properties in the Sarda sheep

Biochemical and molecular characterization of polymorphisms of αs1-casein in Sudanese camel (Camelus dromedarius) milk

Association of the CSN1S1 gene polymorphism with milk composition traits in Jamunapari goat

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