Milk Whey Culture With Propionibacterium freudenreichii ET-3 Is Effective on the Colitis Induced by 2,4,6-Trinitrobenzene Sulfonic Acid in Rats

Abstract

Abstract. This study aimed to evaluate whether milk whey culture with Propinibacterium freudenreichii ET-3 (milk whey culture), which has been reported to have Bifidogenic activity, is effective on the colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) in rats. For the induction of colitis, the colon was clamped and 0.1 M TNBS in 35% ethanol was injected into the luminal side of the clamped portion under pentobarbital anesthesia. From the next day of colitis induction, milk whey culture was administered orally at doses of 1 and 3 g / kg, twice a day for 9 days. On the 10th day, rats were sacrificed and ulcer size was measured. Milk whey culture significantly accelerated the healing of the colitis in a dose-dependent manner, but culture medium did not. To clarify the active substance, the effects of propionic acid and acetic acid contained in milk whey culture was tested. Sodium propionate significantly accelerated the healing of TNBS-induced colitis, but sodium acetate did not. The above results show that milk whey culture may become a useful prebiotic for the therapy of inflammatory bowel disease and that propionic acid may be one of the active substances contained in milk whey culture.

Strain-to-strain differences within lactic and propionic acid bacteria species strongly impact the properties of cheese–A review

“…By contrast, some P. freudenreichii strains produce a surface beta-glucan exopolysaccharide after growth in a dairy-based medium and display no immunomodulatory properties, while the mutational inactivation of this exopolysaccharide capsule leads to anti-inflammatory properties of the mutant strains (Deutsch et al , 2012. Accordingly, pure cultures of P. freudenreichii ET-3 in whey exerted protective effects in experimental colitis in mice (Okada et al 2006;Uchida and Mogami 2005). A pilot clinical study on ulcerative colitis also suggests a beneficial effect of this strain in humans (Suzuki et al 2006).…”

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

“…It has been demonstrated that consumption of either isolated BGS or P. freudenreichii strains ameliorate experimental colitis in mice and human ulcerative colitis [171,[189][190][191][192]. The mechanism proposed for this effect was restoring of microbiota intestinal balance and suppressing inflammatory lymphocyte infiltration.…”

Promising Immunomodulatory Effects of Selected Strains of Dairy Propionibacteria as Evidenced In Vitro and In Vivo

“…Dairy propionibacteria display various probiotic properties either similar to or distinct from those reported for probiotic bifidobacteria and lactic acid bacteria (3). Although an anti-inflammatory potential of a few dairy propionibacterium strains was occasionally suggested in vitro (15) or in animals (22,24,31), no reliable observation was established in terms of strain variability and of specific mechanisms involved. In addition, supplementation with dairy propionibacteria in human randomized, placebo-controlled, double-blind trials has mainly concerned mixtures comprising probiotic bacteria assigned to genera other than Propionibacterium but rarely with propionibacteria alone (16).…”