Millifluidic valves and pumps made of tape and plastic

Amador-Hernandez, Josue U.; Guevara-Pantoja, Pablo E.; Cedillo-Alcantar, Diana F.; Caballero-Robledo, Gabriel A.; Garcia-Cordero, Jose L.

doi:10.1039/d3lc00559c

Cited by 1 publication

(1 citation statement)

References 58 publications

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“…Overall, affordable rapid prototyping methods including 3D printing, xurography, and silicone-based shadow masking are ideally suited to create versatile and affordable platform technologies aimed towards acute cytotoxic risk assessment studies [53,[55][56][57]. We demonstrated in a proof of concept how the proposed prototyping pipelines are feasible to create human cell models for cytotoxic evaluation approaches in environmental (nano-) toxicology, where the ultimate goal may be not expensive and quantitative assaying but simple and affordable pre-screening tools for environmental risk assessment, especially when coupled in the future with unmanned vehicle applications.…”

Section: Discussionmentioning

confidence: 99%

Using Rapid Prototyping to Develop a Cell-Based Platform with Electrical Impedance Sensor Membranes for In Vitro RPMI2650 Nasal Nanotoxicology Monitoring

Vasconez Martinez,

Reihs,

Stuetz

et al. 2024

Biosensors

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Due to advances in additive manufacturing and prototyping, affordable and rapid microfluidic sensor-integrated assays can be fabricated using additive manufacturing, xurography and electrode shadow masking to create versatile platform technologies aimed toward qualitative assessment of acute cytotoxic or cytolytic events using stand-alone biochip platforms in the context of environmental risk assessment. In the current study, we established a nasal mucosa biosensing platform using RPMI2650 mucosa cells inside a membrane-integrated impedance-sensing biochip using exclusively rapid prototyping technologies. In a final proof-of-concept, we applied this biosensing platform to create human cell models of nasal mucosa for monitoring the acute cytotoxic effect of zinc oxide reference nanoparticles. Our data generated with the biochip platform successfully monitored the acute toxicity and cytolytic activity of 6 mM zinc oxide nanoparticles, which was non-invasively monitored as a negative impedance slope on nasal epithelial models, demonstrating the feasibility of rapid prototyping technologies such as additive manufacturing and xurography for cell-based platform development.

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Section: Discussionmentioning

confidence: 99%