Millimeter-Wave-Induced Hypoalgesia in Mice: Dependence on Type of Experimental Pain

Radzievsky, Alexander A.; Gordiienko, Oleg; Cowan, Alan; Алексеев, С. И.; Ziskin, Marvin C.

doi:10.1109/tps.2004.830972

Cited by 19 publications

(21 citation statements)

References 53 publications

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“…The hypoalgesia was most pronounced in the model of chronic non-neuropathic pain, in which a single exposure to MMW results in more than a twofold increase in coldinduced pain resistance. Further experiments demonstrated that: MMWT-induced hypoalgesia is specific, and cannot be reproduced by equivalent heating of the same area of exposure; is power dependent; and its effectiveness is highest when the exposed skin area possesses the greatest neural innervation density [Rojavin et al, 2000;Radzievsky et al, 2004a]. In the present set of experiments, we have determined that MMWT-induced hypoalgesia is also frequencydependent.…”

Section: Discussionmentioning

confidence: 58%

“…A number of experimental studies, including our present experiments, have clarified the possible biological mechanisms of the treatment [Pakhomov et al, 1998b;Logani et al, 2002;Makar et al, 2003;Radzievsky et al, 2004a;Alekseev et al, 2005;Szabo et al, 2006]. Some clinical studies have demonstrated the effectiveness of MMWT under ''double-blind'' conditions [Korpan and Saradeth, 1995;Megdiatov et al, 1995;Radzievsky et al, 1999;Usichenko et al, 2003].…”

Section: Discussionmentioning

confidence: 63%

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Electromagnetic millimeter wave induced hypoalgesia: Frequency dependence and involvement of endogenous opioids

Radzievsky

Gordiienko

Алексеев

et al. 2007

Bioelectromagnetics

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Millimeter wave treatment (MMWT) is based on the systemic biological effects that develop following local skin exposure to low power electromagnetic waves in the millimeter range. In the present set of experiments, the hypoalgesic effect of this treatment was analyzed in mice. The murine nose area was exposed to MMW of "therapeutic" frequencies: 42.25, 53.57, and 61.22 GHz. MMWT-induced hypoalgesia was shown to be frequency dependent in two experimental models: (1) the cold water tail-flick test (chronic non-neuropathic pain), and (2) the wire surface test (chronic neuropathic pain following unilateral constriction injury to the sciatic nerve). Maximum hypoalgesic effect was obtained when the frequency was 61.22 GHz. Other exposure parameters were: incident power density = 13.3 mW/cm(2), duration of each exposure = 15 min. Involvement of delta and kappa endogenous opioids in the MMWT-induced hypoalgesia was demonstrated using selective blockers of delta- and kappa-opioid receptors and the direct ELISA measurement of endogenous opioids in CNS tissue. Possible mechanisms of the effect and the perspectives of the clinical application of MMWT are discussed.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 63%

Electromagnetic millimeter wave induced hypoalgesia: Frequency dependence and involvement of endogenous opioids

Radzievsky

Gordiienko

Алексеев

et al. 2007

Bioelectromagnetics

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“…Radiant heating of the cells failed to reproduce the effect. Finally, in a set of experiments on mouse skin receptors, the tail flick response was observed to decrease with millimeter wave exposure [15,16] in contrast to simple heating.…”

Section: Introductionmentioning

confidence: 95%

“…Civilian Federal Agencies, very few research groups are willing to undertake these studies. Several investigations [4][5][6][7][8][9][10][11][12][13][14][15][16], mainly outside the U.S., have noted significant impact on neuronal activity from modest level millimeter wave exposures (40-130 GHz, 1-100 mW/cm 2 , seconds to minutes) that are not much higher than the Federal Communications Commission-established maximum permissible exposure (MPE) limits of 1 mW/cm 2 for 6 minutes in the 30-300 GHz frequency regime [17]. Synchronization of the firing rate of neurons in the hypothalamus of both rabbit and rat was observed at and below 10 mW/cm 2 [4,5].…”

Section: Introductionmentioning

confidence: 99%

THz in biology and medicine: toward quantifying and understanding the interaction of millimeter- and submillimeter-waves with cells and cell processes

Siegel

Pikov

2010

SPIE Proceedings

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As the application and commercial use of millimeter-and submillimeter-wavelength radiation become more widespread, there is a growing need to understand and quantify both the coupling mechanisms and the impact of this long wavelength energy on biological function. Independent of the health impact of high doses of radio frequency (RF) energy on full organisms, which has been extensively investigated, there exists the potential for more subtle effects, which can best be quantified in studies which examine real-time changes in cellular functions as RF energy is applied. In this paper we present the first real time examination of RF induced changes in cellular activity at absorbed power levels well below the existing safe exposure limits. Fluorescence microscopy imaging of immortalized epithelial and neuronal cells in vitro indicate increased cellular membrane permeability and nanoporation after short term exposure to modest levels (10-50 mW/cm 2 ) of RF power at 60 GHz. Sensitive patch clamp measurements on pyramidal neurons in cortical slices of neonatal rats showed a dramatic increase in cellular membrane permeability resulting either in suppression or facilitation of neuronal activity during exposure to sub-µW/cm 2 of RF power at 60 GHz. Non-invasive modulation of neuronal activity could prove useful in a variety of health applications from suppression of peripheral neuropathic pain to treatment of central neurological disorders.

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“…Experimental laboratory and clinical investigations of biological effects of millimeter waves have used both in vitro and in vivo approaches [3], [5]- [8]. Thus far, there are only a very few papers dealing with dosimetry at millimeter waves.…”

mentioning

confidence: 99%

Numerical and Experimental Millimeter-Wave Dosimetry for In Vitro Experiments

Sauleau

Dréan

Алексеев

et al. 2008

IEEE Trans. Microwave Theory Techn.

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International audienceThis paper provides extensive dosimetry data for in vitro experiments regarding the biological effects of millimeter waves. Two particular frequency ranges have been considered, which are: (1) the 57-64-GHz frequency range dedicated to near-future applications in high-speed wireless communication systems and (2) the discrete frequencies used in millimeter-wave therapy, namely, 42.25, 53.57, and 61.22 GHz. The dielectric properties of keratinocyte cells and culture media were determined using permittivity data of free water and Maxwell's mixture equation. The local specific absorption rate (SAR) distribution within the cell monolayer located in a standard tissue culture plate was computed using the finite-element method and the finite-integration technique. The averaged near-surface SAR for the cell monolayer was determined using both numerical electric-field-based and experimental temperature-based approaches. The SAR was computed taking into account physiological variations of the water content in the keratinocyte cells, as well as variations in the cell monolayer thickness. Experimental and computational results are shown to be in very good agreement

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Millimeter-Wave-Induced Hypoalgesia in Mice: Dependence on Type of Experimental Pain

Cited by 19 publications

References 53 publications

Electromagnetic millimeter wave induced hypoalgesia: Frequency dependence and involvement of endogenous opioids

Electromagnetic millimeter wave induced hypoalgesia: Frequency dependence and involvement of endogenous opioids

THz in biology and medicine: toward quantifying and understanding the interaction of millimeter- and submillimeter-waves with cells and cell processes

Numerical and Experimental Millimeter-Wave Dosimetry for In Vitro Experiments

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