Mimicking the Brain Extracellular Matrix <i>in Vitro</i>: A Review of Current Methodologies and Challenges

Rauti, Rossana; Renous, Noa; Maoz, Ben M.

doi:10.1002/ijch.201900052

Cited by 50 publications

(37 citation statements)

References 134 publications

(329 reference statements)

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“…[9] We have furthermore studied the contributions of the human brain microvascular endothelial cells interfaced with human pericytes and astrocytes to inflammatory processes in 2D cultures and 3D BBB Chip configuration. [11] While there is a need for advanced brain in vitro models, [12,13] the characterization of the metabolic and proteomic phenotypes of these individual NVU cell types in conventional cell cultures is still lacking, even though this format is far more widely applied for in vitro studies and high-throughput screening. Therefore, this study is based on data generated from separated conventional 2D cultures of each of the NVU cell types.…”

Section: The Functional State Of the Neurovascular Unit (Nvu) Composmentioning

confidence: 99%

Proteomic and Metabolomic Characterization of Human Neurovascular Unit Cells in Response to Methamphetamine

et al. 2020

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The functional state of the neurovascular unit (NVU), composed of the blood–brain barrier and the perivasculature that forms a dynamic interface between the blood and the central nervous system (CNS), plays a central role in the control of brain homeostasis and is strongly affected by CNS drugs. Human primary brain microvascular endothelium, astrocyte, pericyte, and neural cell cultures are often used to study NVU barrier functions as well as drug transport and efficacy; however, the proteomic and metabolomic responses of these different cell types are not well characterized. Culturing each cell type separately, using deep coverage proteomic analysis and characterization of the secreted metabolome, as well as measurements of mitochondrial activity, the responses of these cells under baseline conditions and when exposed to the NVU‐impairing stimulant methamphetamine (Meth) are analyzed. These studies define the previously unknown metabolic and proteomic profiles of human brain pericytes and lead to improved characterization of the phenotype of each of the NVU cell types as well as cell‐specific metabolic and proteomic responses to Meth.

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Section: The Functional State Of the Neurovascular Unit (Nvu) Composmentioning

confidence: 99%

Proteomic and Metabolomic Characterization of Human Neurovascular Unit Cells in Response to Methamphetamine

et al. 2020

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“…As a major component of the brain ECM, HA plays a significant role in maintaining homeostasis in neuronal tissue through influencing cell migration, proliferation, differentiation, and other cell behaviors [ 33 , 34 , 35 ]. Cell function is also influenced by the molecular weight (Mw) of HA, as low (~80 to 800 kDa) and high Mw (>1600 kDa) forms of HA elicit different cell behaviors [ 36 , 37 , 38 , 39 ].…”

Section: Biological Activity Of Ha In the Cnsmentioning

confidence: 99%

Hyaluronic Acid Biomaterials for Central Nervous System Regenerative Medicine

Jensen

Holloway

Stabenfeldt

2020

Cells

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Hyaluronic acid (HA) is a primary component of the brain extracellular matrix and functions through cellular receptors to regulate cell behavior within the central nervous system (CNS). These behaviors, such as migration, proliferation, differentiation, and inflammation contribute to maintenance and homeostasis of the CNS. However, such equilibrium is disrupted following injury or disease leading to significantly altered extracellular matrix milieu and cell functions. This imbalance thereby inhibits inherent homeostatic processes that support critical tissue health and functionality in the CNS. To mitigate the damage sustained by injury/disease, HA-based tissue engineering constructs have been investigated for CNS regenerative medicine applications. HA’s effectiveness in tissue healing and regeneration is primarily attributed to its impact on cell signaling and the ease of customizing chemical and mechanical properties. This review focuses on recent findings to highlight the applications of HA-based materials in CNS regenerative medicine.

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“…Therefore, the dECM has recently become attractive, as the dECM has the potential to preserve the original protein composition of the brain by adapting the native tissue. Therefore, the “brain dECM” has emerged as an approach to recapitulating a brain-specific microenvironment, particularly with regard to protein components and biocompatibility with neural cells [ 96 , 97 ].…”

Section: Materials For Neural Mps Designmentioning

confidence: 99%

Microphysiological Systems for Neurodegenerative Diseases in Central Nervous System

Bae

Jang

et al. 2020

Micromachines

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Neurodegenerative diseases are among the most severe problems in aging societies. Various conventional experimental models, including 2D and animal models, have been used to investigate the pathogenesis of (and therapeutic mechanisms for) neurodegenerative diseases. However, the physiological gap between humans and the current models remains a hurdle to determining the complexity of an irreversible dysfunction in a neurodegenerative disease. Therefore, preclinical research requires advanced experimental models, i.e., those more physiologically relevant to the native nervous system, to bridge the gap between preclinical stages and patients. The neural microphysiological system (neural MPS) has emerged as an approach to summarizing the anatomical, biochemical, and pathological physiology of the nervous system for investigation of neurodegenerative diseases. This review introduces the components (such as cells and materials) and fabrication methods for designing a neural MPS. Moreover, the review discusses future perspectives for improving the physiological relevance to native neural systems.

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Mimicking the Brain Extracellular Matrix in Vitro: A Review of Current Methodologies and Challenges

Cited by 50 publications

References 134 publications

Proteomic and Metabolomic Characterization of Human Neurovascular Unit Cells in Response to Methamphetamine

Proteomic and Metabolomic Characterization of Human Neurovascular Unit Cells in Response to Methamphetamine

Hyaluronic Acid Biomaterials for Central Nervous System Regenerative Medicine

Microphysiological Systems for Neurodegenerative Diseases in Central Nervous System

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