Mineral phosphate solubilization activity of gluconacetobacter diazotrophicus under P-limitation and plant root environment

Crespo, Juan Manuel; Boiardi, J. L.; Luna, María Flavia

doi:10.4236/as.2011.21003

Cited by 23 publications

(21 citation statements)

References 24 publications

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“…G. diazotrophicus synthetize gluconic acid by the extracellular oxidation of the glucose by the action of the enzyme glucose dehydrogenase (GDH‐PQQ), localized in the perisplasmic space , leading to the production of gluconic acid. This mild non‐corrosive acid can, besides lowering the pH, promote chelation and exchange reactions and has been associated with phosphate and zinc solubilisation/chelation by G. diazotrophicus . Saravanan et al .…”

Section: The Gluconacetobacter Genusmentioning

confidence: 99%

Nitrogen fixing bacteria in the family Acetobacteraceae and their role in agriculture

Reis

Teixeira

2015

J. Basic Microbiol.

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For centuries, the Acetobacteraceae is known as a family that harbors many species of organisms of biotechnological importance for industry. Nonetheless, since 1988 representatives of this family have also been described as nitrogen fixing bacteria able to plant growth promotion by a variety of mechanisms. Nitrogen fixation is a biological process that guarantees that the atmospheric N2 is incorporated into organic matter by several bacterial groups. Most representatives of this group, also known as diazotrophic, are generally associated with soil rhizosphere of many plants and also establishing a more specific association living inside roots, leaves, and others plants tissues as endophyte. Their roles as plant growth-promoting microorganisms are generally related to increase in plant biomass, phosphate and other mineral solubilization, and plant pathogen control. Here, we report many of these plant growth-promoting processes related to nitrogen fixing species already described in Acetobacteraceae family, especially Gluconacetobacter diazotrophicus and their importance to agriculture. In addition, a brief review of the state of art of the phylogenetics, main physiological and biochemical characteristics, molecular and functional genomic data of this group of Acetobacteraceae is presented.

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Section: The Gluconacetobacter Genusmentioning

confidence: 99%

Nitrogen fixing bacteria in the family Acetobacteraceae and their role in agriculture

Reis

Teixeira

2015

J. Basic Microbiol.

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“…Indole-3-acetic acid (IAA) and gibberellins A1 and A3 have been found to be produced by G. diazotrophicus, both phytohormones are critical for normal plant growth and development [28,52,53]. G. diazotrophicus has also been found to have phosphorous and zinc solubilisation capabilities [22,[54][55][56][57]. Zinc and phosphorus, micro and macronutrients, respectively, are important to the growth, development, and yield of many plants.…”

Section: Mechanism Aiding Plant Growth Promotionmentioning

confidence: 99%

Research Progress and Perspectives of Nitrogen Fixing Bacterium,Gluconacetobacter diazotrophicus, in Monocot Plants

Eskin

Vessey

Tian

2014

International Journal of Agronomy

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Gluconacetobacter diazotrophicus is a nitrogen fixing bacterium originally found in monocotyledon sugarcane plants in which the bacterium actively fixes atmosphere nitrogen and provides significant amounts of nitrogen to plants. This bacterium mainly colonizes intercellular spaces within the roots and stems of plants and does not require the formation of the complex root organ like nodule. The bacterium is less plant/crop specific and indeed G. diazotrophicus has been found in a number of unrelated plant species. Importantly, as the bacterium was of monocot plant origin, there exists a possibility that the nitrogen fixation feature of the bacterium may be used in many other monocot crops. This paper reviews and updates the research progress of G. diazotrophicus for the past 25 years but focuses on the recent research development.

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“…Many reports show that some soil bacteria solubilize insoluble minerals through the production of acids, increasing the availability of P and other nutrients to plants in deficient soils [2]. Several bacterial genera have been reported to express "mineral phosphate solubilizing" (MPS) capacity such as Pseudomonas, Bacillus, Rhizobium, Burkholderia, Achromobacter, Agrobacterium, Microccocus, Aereobacter, Flavobacterium, Erwinia and Gluconacetobacter [3][4][5]. It is generally accepted that the expression of a direct extracellular oxidative pathway is the principal mechanism for MPS bacteria [5,[6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase

Bernabeu

Garcia

Ferreyra

et al. 2016

BMRJ

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Authors' contributionsThis manuscript is the result of teamwork. Author PRB designed all the experiments and managed the analysis and discussion of results. Author SSG contributed with experiments in flasks and gluconic acid and phosphorous determinations and managed literature searches. Author MLG carried out the supervision of experiments in flasks and bioreactors in relation with enzyme activities measures. Authors GGF and VIG realized all the experiments in bioreactors and enzyme activities measures. Author JLB contributed with the discussion of results, organization and planning of the manuscript.Author MFL developed the experimental design, supervised the experiments and managed the analysis and discussion of results. All authors read and approved the final manuscript. Article InformationABSTRACT Aims: The objective of this work was to provide knowledgement about the mechanism and regulation of the mineral phosphate solubilization in Burkholderia tropica. To this end, the expression of the direct extracellular oxidative pathway in B. tropica was studied using different culture approaches. Bernabeu et al.; BMRJ, 13(4): 1-8, 2016; Article no.BMRJ.24405 2 Study Design: Plate assays and batch cultures in flasks and bioreactor were carried out in this study with B. tropica Mto-293 like target organism. The experiments were achieved at least three times with two repetitions per time. Place and Duration of Study: Departamento de Química, Centro de Investigación y Desarrollo en Fermentaciones Industriales, UNLP, CCT-La Plata-CONICET, between November 2014-2015. Methodology: Qualitative plate assays with different Carbon sources were carried out for the evaluation of Mineral Phosphate Solubilization phenotype. Batch cultures in flasks were carried out with different Carbon, Phosphorus and Nitrogen sources to determine quantitatively soluble phosphorus, gluconic acid and other ketoacids in the supernatants, and also PQQ-linked glucose and gluconate dehydrogenase activities in whole cells. Original Research ArticleCultures with some of the conditions mentioned before were carried out in bioreactor specifically to control pH. Results: This organism was able to produce significant amounts of gluconic acid via the expression of a PQQ-GDH and also showed a significant activity of GaDH. However, the direct oxidative pathway was only observed under conditions of Phosphorus starvation and/or Nitrogen fixation. Conclusion:The Mineral Phosphate Solubilization phenotype for B. tropica can be ascribed to the expression of the direct oxidative pathway which involves the expression of an active PQQ-linked glucose dehydrogenase. Nevertheless, this pathway is not expressed constitutively in this bacterium. Environmental conditions, like low P and N availability, led to an active extracellular glucose oxidation. Therefore, mineral phosphate solubilization in B. tropica involves an inducible pyrroloquinoline quinone-linked glucose dehydrogenase. These findings may contribute to the use of this bacterium as plant growth promoting bacte...

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Mineral phosphate solubilization activity of gluconacetobacter diazotrophicus under P-limitation and plant root environment

Cited by 23 publications

References 24 publications

Nitrogen fixing bacteria in the family Acetobacteraceae and their role in agriculture

Nitrogen fixing bacteria in the family Acetobacteraceae and their role in agriculture

Research Progress and Perspectives of Nitrogen Fixing Bacterium,Gluconacetobacter diazotrophicus, in Monocot Plants

Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase

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