Mineral profiling of ostrich (Struthio camelus) seminal plasma and its relationship with semen traits and collection day

Smith, A.M.J.; Bonato, Maud; Dzama, K.; Malecki, Irek; Cloete, S.W.P.

doi:10.1016/j.anireprosci.2018.04.004

Cited by 9 publications

(7 citation statements)

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“…; Juyena & Stelletta ; Smith et al . ). In particular, Mg 2+ has a vital function in modulating the regulation of K + (Na‐K pump) and Ca 2+ (Owczarzy et al .…”

Section: Discussionmentioning

confidence: 97%

“… ; Smith et al . ) as well as playing an essential role in enzymatic reactions involving anaerobic glycolysis and energy release from ATP for sperm activities (Wong et al . ; Asghari et al .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, although the proportion of healthy sperm in T-M-EY were higher than that in T-EY during 6 days, it reduced suddenly after day 6 and gained a similar value with T-EY extender on day 10. These factors may help to explain that T-M-EY extenders contain several mineral cations, such as Na + , K + , Mg 2+ , Ca 2+ , which are the main ions of seminal plasma, whose important functions are to maintain osmotic balance, form parts of principal enzymes relating to sperm metabolism and sperm function (C ß evik et al 2007;Juyena & Stelletta 2012;Smith et al 2018). In particular, Mg 2+ has a vital function in modulating the regulation of K + (Na-K pump) and Ca 2+ (Owczarzy et al 2008;Smith et al 2018) as well as playing an essential role in enzymatic reactions involving anaerobic glycolysis and energy release from ATP for sperm activities (Wong et al 2001;Asghari et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…; Juyena & Stelletta ; Smith et al . ). In previous studies, although canine seminal plasma has been found to be beneficial for chilled sperm plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential, it had detrimental effects on sperm motility (Rota et al .…”

Section: Introductionmentioning

confidence: 97%

“…It has crucial functions in sperm ejaculation and sperm survival in the female genital tract. The role of mineral ions is essential for maintaining osmotic balance, forming parts of principal enzymes relating to sperm metabolism and sperm function (C ß evik et al 2007;Juyena & Stelletta 2012;Smith et al 2018). In previous studies, although canine seminal plasma has been found to be beneficial for chilled sperm plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential, it had detrimental effects on sperm motility (Rota et al 1995;Treulen et al 2012;Hori et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

Nguyen

Ponchunchoovong

Kupittayanant

et al. 2019

Veterinary Medicine & Sci

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The reduction of spermatozoa survival time is a major problem of canine chilled sperm for artificial insemination. The aim of the study was to improve the quality of canine chilled sperm during storage time. We therefore, evaluated the effects of eight treatments with different levels of soybean lecithin concentration (1, 3 and 5%) and egg yolk (20%) in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extender on chilled canine sperm quality during 10 days of storage. The sperm motility was analysed by computer‐assisted sperm analysis (CASA), whereas plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential parameters were determined using a fluorescent staining combination of propidium iodide (PI), Hoechst 33342 (H342), fluorescein isothiocyanate‐conjugated Pisum sativum agglutinin (FITC‐PSA) and 5,5′,6,6′‐tetrachloro‐1,1′,3,3′‐tetraethylbenzimidazolyl‐carbocyanine iodide (JC‐1) by confocal laser scanning microscope. The results showed that egg yolk was found to be better than soybean lecithin in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extender for maintaining the quality of chilled canine sperm within 10 days of storage (P < 0.05). Although egg yolk in Tris‐citric‐fructose extender could maintain the motility better than other extenders, egg yolk in Tris‐citric‐fructose‐mineral salts extender was the highest in intact plasma membrane, intact acrosome membrane and high mitochondrial membrane potential (P < 0.05). In contrast, the sperm quality of soybean lecithin in Tris‐citric‐fructose‐mineral salts extender was lower than that of soybean lecithin in Tris‐citric‐fructose extender, and soybean lecithin 1% was greater than soybean lecithin 3% and 5% in plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential (P < 0.05). In conclusion, soybean lecithin cannot replace egg yolk in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extenders, and egg yolk in Tris‐citric‐fructose‐mineral salts extender is superior to other extenders in chilling canine sperm.

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“…; Juyena & Stelletta ; Smith et al . ). In particular, Mg 2+ has a vital function in modulating the regulation of K + (Na‐K pump) and Ca 2+ (Owczarzy et al .…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%