Mineral trioxide aggregate‐induced AMPK activation stimulates odontoblastic differentiation of human dental pulp cells

Kim, Yun Joong; Kim, Wonjae; Bae, Sun-Woong; Yang, Sunmi; Park, Sam-Young; Kim, Seon-Mi; Jung, Ji‐Yeon

doi:10.1111/iej.13460

Cited by 7 publications

(5 citation statements)

References 50 publications

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“…DMP 1 is secreted by fully differentiated odontoblasts and plays a fundamental role in dentinogenesis, mediating the mineralization of peritubular dentin and collagen fibrils from intertubular dentin [48,[51][52][53]. Unlike previous investigations in which DMP 1 was expressed in response to calcium hydroxide and MTA [48,54], in the present study, DMP 1 was detected only in 21-day samples treated with pAsp, suggesting that only in this group were the odontoblast-like cells functionally mature. The faster cell differentiation can be attributed to the presence of pAsp in the exposure site, which would act on undifferentiated pulp cells, stimulating their migration, proliferation, and differentiation into odontoblastlike cells, upregulating the expression of proteins related to dentinogenesis, such as DMP 1 and promoting the deposition of endogenous calcium ions through chelation [22,23,25,55].…”

Section: Discussioncontrasting

confidence: 74%

Poly(Aspartic Acid) Promotes Odontoblast-like Cell Differentiation in Rat Molars with Exposed Pulp

dos Santos,

Habelitz,

Nascimento

et al. 2023

JFB

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In recent years, alternative pulpal therapies targeting dentinogenesis signaling pathways using different peptides have been investigated. The aim of this study was to verify the effectiveness of poly(aspartic acid), pAsp, in dentin regeneration using an animal model. Methods: Mechanical pulp exposure was performed in the upper molars of 56 Wistar rats, randomly divided as follows (n = 14): control (no treatment); MTA group—pulp capping with mineral trioxide aggregate (MTA Angelus); pAsp group—application of 20 μL of pAsp solution (25 mg·mL−1); MTA+pAsp group—application of MTA mixed with pAsp (5:1 by mass). Animals were euthanized after 7 or 21 days. Histological sections were submitted to hematoxylin-eosin and Brown and Brenn staining and immunohistochemical analysis for osteopontin (OPN) and dentin matrix protein 1 (DMP 1). Results: At 7 days, an acute inflammatory infiltrate and the presence of disorganized mineralized tissue were observed in all groups. At 21 days, the quality and thickness of the reparative dentin in treated groups were superior to the control, and bacterial contamination was observed in two MTA-pAsp specimens. While all treated groups showed intense immunostaining for OPN at 21 days, only the pAsp group expressed DMP 1, indicating the presence of fully differentiated odontoblast-like cells. Conclusion: Poly(aspartic) acid promoted dentin regeneration in rat molars in the absence of an additional calcium source and may be an alternative to MTA as a pulp-capping agent.

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Section: Discussioncontrasting

confidence: 74%

Poly(Aspartic Acid) Promotes Odontoblast-like Cell Differentiation in Rat Molars with Exposed Pulp

dos Santos,

Habelitz,

Nascimento

et al. 2023

JFB

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“…DSPP is mainly expressed in odontoblast and closely related to tooth development and biomineralization, and DMP-1 is a non-collagenous extracellular matrix protein of dentin. Therefore, DSPP and DMP-1 are usually used as the markers of odontoblastic differentiation ( 19 , 22 , 23 ). OCN is one of the differentiation markers from osteoblasts to mineralization stage, and RUNX2 is the key transcription factor for osteogenic differentiation at early stage ( 24 , 25 ).…”

Section: Resultsmentioning

confidence: 99%

MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

Huang

2021

IJSC

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Background and Objectives MiR-148a-3p has been reported to regulate the differentiation of marrow stromal cell osteoblast. In this study, whether miR-148a-3p regulated the odontoblastic differentiation of human dental pulp stem cells (hDPSCs) or not was explored. Methods and Results The hDPSCs were isolated and identified via flow cytometry. Targets of miR-148a-3p were identified via bioinformatics and dual-luciferase reporter assay. After the cell was cultured in the odontogenic differentiation medium or infected, cell viability, invasion, and odontoblastic differentiation were detected via MTT, transwell, and Alizarin Red S staining, respectively. The miR-148a-3p, Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions were determined by RT-qPCR and Western blot. The hDPSCs odontoblastic differentiation downregulated the miR-148a-3p expression and upregulated Wnt1 expression. Wnt1 was determined as the target for miR-148a-3p. MiR-148a-3p mimic and siWnt1 suppressed the cell viability, invasion, and odontoblastic differentiation of hDPSCs and inhibited the Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions. In contrast, miR-148a-3p inhibitor and overexpressed Wnt1 promoted the cell viability, invasion, and odontoblastic differentiation of hDPSCs, and upregulated the Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions. Also, miR-148a-3p mimic and inhibitor reversed the effects of Wnt1 overexpression and siWnt1. Conclusions MiR-148a-3p modulated the invasion and odontoblastic differentiation of hDPSCs through the Wnt1/β-catenin pathway.

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“…Conversely, the inhibition of autophagy by the use of 3‐MA resulted in a notable decrease in the expression levels of dentin sialophosphoprotein and dentin matrix protein 1 27 . The administration of mineral trioxide aggregate following cavity preparation in the molars of rats was seen to induce an upregulation of dentin matrix protein 1 and dentin sialophosphoprotein, as well as an increase in the levels of LC3II and p62, along with an enhancement in the phosphorylation of AMPK 28 . A study in autophagy‐impaired mice in epitel‐derived tissues shows that autophagy plays an important role in ameloblast differentiation 29…”

Section: Autophagy and Odontogenesismentioning

confidence: 97%

“…27 The administration of mineral trioxide aggregate following cavity preparation in the molars of rats was seen to induce an upregulation of dentin matrix protein 1 and dentin sialophosphoprotein, as well as an increase in the levels of LC3II and p62, along with an enhancement in the phosphorylation of AMPK. 28 A study in autophagy-impaired mice in epitel-derived tissues shows that autophagy plays an important role in ameloblast differentiation. 29 Autophagy has the potential to induce the migration of dental pulp stem cells and facilitate the process of pulp regeneration.…”

Section: Autophagy and Odontog Ene S Ismentioning

confidence: 99%

The role of autophagy in odontogenesis, dental implant surgery, periapical and periodontal diseases

İnan,

Barış

2024

J Cellular Molecular Medi

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Autophagy is a cellular process that is evolutionarily conserved, involving the sequestration of damaged organelles and proteins into autophagic vesicles, which subsequently fuse with lysosomes for degradation. Autophagy controls the development of many diseases by influencing apoptosis, inflammation, the immune response and different cellular processes. Autophagy plays a significant role in the aetiology of disorders associated with dentistry. Autophagy controls odontogenesis. Furthermore, it is implicated in the pathophysiology of pulpitis and periapical disorders. It enhances the survival, penetration and colonization of periodontal pathogenic bacteria into the host periodontal tissues and facilitates their escape from host defences. Autophagy plays a crucial role in mitigating exaggerated inflammatory reactions within the host's system during instances of infection and inflammation. Autophagy also plays a role in the relationship between periodontal disease and systemic diseases. Autophagy promotes wound healing and may enhance implant osseointegration. This study reviews autophagy's dento‐alveolar effects, focusing on its role in odontogenesis, periapical diseases, periodontal diseases and dental implant surgery, providing valuable insights for dentists on tooth development and dental applications. A thorough examination of autophagy has the potential to discover novel and efficacious treatment targets within the field of dentistry.

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Mineral trioxide aggregate‐induced AMPK activation stimulates odontoblastic differentiation of human dental pulp cells

Abstract: Mineral trioxide aggregate-induced AMPK activation stimulates odontoblastic differentiation of human dental pulp cells.

Cited by 7 publications

References 50 publications

Poly(Aspartic Acid) Promotes Odontoblast-like Cell Differentiation in Rat Molars with Exposed Pulp

Poly(Aspartic Acid) Promotes Odontoblast-like Cell Differentiation in Rat Molars with Exposed Pulp

MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

The role of autophagy in odontogenesis, dental implant surgery, periapical and periodontal diseases

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