Mineralization and immobilization of nitrogen in fumigated soil and the measurement of microbial biomass nitrogen

Shen, Sida; Pruden, G.; Jenkinson, D. S.

doi:10.1016/0038-0717(84)90049-x

Cited by 278 publications

(83 citation statements)

References 16 publications

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“…This expression had been derived from the relationship between the amount of EN and the flush of N caused by fumigation (FN) in the FI method in a wide range of soils. For the FI method, the soil microbial biomass N was calculated from the equation BN=FN/KN where KN=0.68 (Shen et al 1984). Recently, Jenkinson (1988) has proposed a new KN value of 0.57.…”

Section: Methodsmentioning

confidence: 99%

Microbial biomass nitrogen and mineralization-immobilization processes of nitrogen in soils incubated with various organic materials

Aoyama

Nozawa

1993

Soil Science and Plant Nutrition

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Section: Methodsmentioning

confidence: 99%

Microbial biomass nitrogen and mineralization-immobilization processes of nitrogen in soils incubated with various organic materials

Aoyama

Nozawa

1993

Soil Science and Plant Nutrition

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“…Cylinders were hand weeded and thinned to Microbial biomass N was determined by the chloroform fumigation-incubation method of Jenkinson and Powlson (1976) on moist, sieved (2 mm) soil adjusted to 55% watetholding capacity. The formula of Shen et al (1984) …”

mentioning

confidence: 99%

Dynamics of ¹⁵N in two soil–plant systems following incorporation of 10% bloom and full bloom field pea

Jans-Hammermeister¹,

McGill²,

Jensen³

1994

Can. J. Soil. Sci.

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“…However, the new N C ) is less than 7.5; calculated without (A) or with (B) correction for residual mineralization of non-biomass constituents in the fumigated soil and by the standard methods (C), (Jenkinson 1988). Data recalculated from Ayanaba et al (1976) (D), Adams 1983 ( ) andShen et al 1984 (+). B C for A and B calculated according to Horwath et al (1996) calculation greatly extends the range of soils for which biomass C:N and N can be determined by the CFI method and is robust even when fumigation is followed by net immobilization of N.…”

Section: Resultsmentioning

confidence: 99%

“…In fumigation-incubation, N mineralized to ammonium in a 10-d incubation following chloroform fumigation is measured (Jenkinson and Powlson 1976;Shen et al 1984). In fumigation-extraction (Brookes et al 1985), the materials released by fumigation are immediately extracted in K 2 SO 4 solution.…”

mentioning

confidence: 99%

Measurement of microbial biomass N:C by chloroform fumigation-incubation

Harris¹,

Voroney²,

Paul³

1997

Can. J. Soil. Sci.

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. 1997. Measurement of microbial biomass N:C by chloroform fumigation-incubation. Can. J. Soil Sci. 77: 507-514. We present a calculation for soil microbial biomass N:C ratio determined from a 10-d incubation following chloroform fumigation. The calculation is based on a mathematical model of the N content of the pre-and post-fumigation soil microbial biomass and the growth yield of the biomass that develops after fumigation. Biomass N is calculated from the N:C ratio and biomass C. The mineralization of bacteria and fungi, with different N contents, added to fumigated soils was used to establish the model parameters. The model was tested against an independent set of measurements and considers two assumptions: 1) The ratio of N:C mineralized from killed biomass is equal to the ratio of N:C mineralized from soil nonbiomass constituents. 2) More realistically, the N and C mineralization in the fumigated soil, from sources other than killed biomass, is a residual fraction of the N and C mineralization in the unfumigated soil. Biomass C:N ratios calculated without a control correction (assumption 1) were, on average, 20% wider than corrected values (assumption 2). Biomass N calculated as the product of N:C and biomass C was compared with published values for several data sets. The new calculation method was robust even when net immobilization of N followed fumigation.Key words: Soil microbial biomass, nitrogen, chloroform fumigation, C:N ratio Harris, D., Voroney, R. P. and Paul, E. A. 1997. Mesure du rapport N:C de la biomasse microbienne par fumigation au chloroforme et incubation. Can. J. Soil Sci. 77: 507-514. Nous présentons une formule de calcul de rapport N:C de la biomasse microbienne (BM) du sol après fumigation au chloroforme suivie d'une période d'incubation de 10 jours. Les calculs sont basés sur un modèle mathématique de la teneur en N de la BM avant et après la fumigation et de l'accroissement de la BM observé après la fumigation. Le N de la BM est calculé à partir du rapport N:C et du C de la biomasse. La minéralisation des bactéries et des champignons microscopiques, de teneurs azotées différentes, incorporés aux sols fumigés est utilisée pour établir les paramètres du modèle. Le modèle, testé sur un ensemble indépendant de mesures, examine deux hypothèses : 1) le rapport N:C minéralisé à partir de la biomasse tuée est égale à celui obtenu pour les composantes du sol autre que la biomasse et 2) de façon plus réaliste, la minéralisation de N et de C dans le sol fumigé, à partir de matières autres que la biomasse est une fraction résiduelle de celle mesurfée dans le sol fumigé. Les rapports C:N de la biomasse microbienne calculés sans correction (hypothèse 1 temoins) étaient en moyenne 20 % plus larges que les valeurs corrigées (hypothèse 2). Le N de biomasse microbienne calculé comme étant le produit de N:C et du C de la biomasse est comparé aux valeurs publiées pour plusieurs ensemble de données. Il appert que la nouvelle méthode de calcul est valable même lorsque la fumigation est suivie d'une immo...

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Mineralization and immobilization of nitrogen in fumigated soil and the measurement of microbial biomass nitrogen

Cited by 278 publications

References 16 publications

Microbial biomass nitrogen and mineralization-immobilization processes of nitrogen in soils incubated with various organic materials

Microbial biomass nitrogen and mineralization-immobilization processes of nitrogen in soils incubated with various organic materials

Dynamics of ¹⁵N in two soil–plant systems following incorporation of 10% bloom and full bloom field pea

Measurement of microbial biomass N:C by chloroform fumigation-incubation

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Mineralization and immobilization of nitrogen in fumigated soil and the measurement of microbial biomass nitrogen

Cited by 278 publications

References 16 publications

Microbial biomass nitrogen and mineralization-immobilization processes of nitrogen in soils incubated with various organic materials

Microbial biomass nitrogen and mineralization-immobilization processes of nitrogen in soils incubated with various organic materials

Dynamics of 15N in two soil–plant systems following incorporation of 10% bloom and full bloom field pea

Measurement of microbial biomass N:C by chloroform fumigation-incubation

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Dynamics of ¹⁵N in two soil–plant systems following incorporation of 10% bloom and full bloom field pea