Mineralized DNA-collagen complex-based biomaterials for bone tissue engineering

James, Bryan D.; Guerin, Paxton; Iverson, Zion; Allen, Josephine B.

doi:10.1016/j.ijbiomac.2020.06.126

Cited by 20 publications

(21 citation statements)

References 75 publications

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“…Exclusion of ion diffusion does not guarantee that capillary action plays a role in the intrafibrillar mineralization process. Nevertheless, capillary action has been regarded as one of the major mechanisms to date to induce intrafibrillar mineralization of collagen, although other mechanisms may have also been involved [ 1 , 24 , 28 , 55 , 56 , 57 ].…”

Section: Intrafibrillar Mineralization Mechanismsmentioning

confidence: 99%

Biomineralization of Collagen-Based Materials for Hard Tissue Repair

Wei

2021

IJMS

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Hydroxyapatite (HA) reinforced collagen fibrils serve as the basic building blocks of natural bone and dentin. Mineralization of collagen fibrils play an essential role in ensuring the structural and mechanical functionalities of hard tissues such as bone and dentin. Biomineralization of collagen can be divided into intrafibrillar and extrafibrillar mineralization in terms of HA distribution relative to collagen fibrils. Intrafibrillar mineralization is termed when HA minerals are incorporated within the gap zone of collagen fibrils, while extrafibrillar mineralization refers to the minerals that are formed on the surface of collagen fibrils. However, the mechanisms resulting in these two types of mineralization still remain debatable. In this review, the evolution of both classical and non-classical biomineralization theories is summarized. Different intrafibrillar mineralization mechanisms, including polymer induced liquid precursor (PILP), capillary action, electrostatic attraction, size exclusion, Gibbs-Donnan equilibrium, and interfacial energy guided theories, are discussed. Exemplary strategies to induce biomimetic intrafibrillar mineralization using non-collagenous proteins (NCPs), polymer analogs, small molecules, and fluidic shear stress are discussed, and recent applications of mineralized collagen fibers for bone regeneration and dentin repair are included. Finally, conclusions are drawn on these proposed mechanisms, and the future trend of collagen-based materials for bone regeneration and tooth repair is speculated.

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Section: Intrafibrillar Mineralization Mechanismsmentioning

confidence: 99%

Biomineralization of Collagen-Based Materials for Hard Tissue Repair

Wei

2021

IJMS

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“…In the last two decades, the use of DNA as a functional biomaterial has been highlighted for numerous applications that prove its potential as an active component in chemistry, physics, computer science, medicine, and nanobiotechnology due to its ability to self‐assemble into a wide range of pre‐engineered forms by complementary base pairing, in addition to low synthesis cost and high biocompatibility 1,2,4,5 . Some assembled DNA‐based structures, primarily referred to as DNA “origami nanostructures,” have a high ability to perform complex cellular activities, such as drug delivery for cancer and enzyme replacement therapy 6‐8 .…”

Section: Introductionmentioning

confidence: 99%

“…Some assembled DNA‐based structures, primarily referred to as DNA “origami nanostructures,” have a high ability to perform complex cellular activities, such as drug delivery for cancer and enzyme replacement therapy 6‐8 . Nowadays, several newly designed nanostructures and nanodevices have been able to use the properties of DNA and functionalize them as biosensors and complex nanoscale structures 1,4,9‐11 …”

Section: Introductionmentioning

confidence: 99%

“…The biomedical use of DNA in regenerative medicine has also been prominent in recent years 2,4,10 . However, much of the effort focused mainly on the development of DNA nanostructures as drug delivery systems due to the lowest antigenicity of DNA as compared to other macromolecules and the ability of cytokines to be stacked between DNA bases 5,6,12‐14 .…”

Section: Introductionmentioning

confidence: 99%

“…Our group's studies have demonstrated the high biocompatibility and excellent controlled drug release properties of DNA‐based polymeric adhesive films 7,9 . However, no reports have been found so far on the construction of cell growth scaffolds based on DNA for wound healing, which could improve tissue regeneration once its ability to promote cell proliferation and differentiation has been recently investigated 4,10,11 …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tailoring the growth and proliferation of human dermal fibroblasts by DNA‐based polymer films for skin regeneration

Jayme

Souza

Fernandes

et al. 2021

J Biomedical Materials Res

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The use of DNA as a functional biomaterial for therapeutic, diagnostic, and drug delivery applications has been prominent in recent years, but its use as a scaffold for tissue regeneration is still limited. This study aimed to evaluate the biocompatibility and interaction of DNA‐based polymeric films (DNA‐PFs) with primary human fibroblasts (PHF) for regenerative medicine and wound healing purposes. The morphological characterization of the films was performed by scanning electron microscopy, SEM–energy‐dispersive X‐ray spectroscopy, and atomic force microscopy analysis. Cell viability, cell cycle kinetics, oxidative stress, and migration studies were carried out at 48 and 72 hr of incubation and compared to control cells. Cell adhesion was impaired in the first 24 hr, DNA‐PFs with higher concentrations of DNA (1.0 and 2.0 g/L) this effect was not seen in DNA‐PFs (0.5 g/L), explained by the difference in topography and roughness of DNA‐PFs, but it was overcome after 48 hr of incubation. PHF seeded on DNA films showed higher proliferation and migration rates than the control after 48 hr of incubation, with the maintenance of cell morphology and lower cytotoxicity and oxidative stress during the evaluation time. Therefore, these results indicate that DNA‐PFs are highly biocompatible and provide a suitable microenvironment for dermal fibroblasts to maintain their activity, helping build new and more complex biomaterials suitable for future tissue repair applications.

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DNA‐Based Materials Inspired by Natural Extracellular DNA

Qin

Wang

Zhang

et al. 2023

Adv Funct Materials

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The advent of biotechnology has expedited the understanding of the biochemistry of deoxyribonucleic acids (DNA). In the past, DNA are thought to be present only in cell nucleus as bearers of the genetic code. With the identification of extracellular DNA in circulating body fluids, DNA are now utilized, at least experimentally, for diagnosis and treatment of diseases. Extracellular DNA of host origin trigger immune responses, and are closely linked to autoimmune disease, cancer-related inflammation, bacteria adhesion and thrombosis. Recent advancements in DNA nanotechnology have led to the development of a series of DNA-based materials for treating diseases because of their structural programmability. Current discussions on biosafety and immunogenicity of artificial DNA materials are insufficient. This issue severely restricts the clinical translation of these novel biotechnologies. The present review attempts to bridge the gap between natural extracellular DNA and their derivatives, DNA-based materials. The pathological attributes of endogenous extracellular DNA motivate the design of targeting DNA materials. In addition, the fate of exogenous DNA in the host inspires the optimization of DNA materials in reducing immune rejection. These bioinspired strategies provide the blueprint for utilizing DNA materials in the management of diseases that are currently challenging to diagnose or treat.

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Mineralized DNA-collagen complex-based biomaterials for bone tissue engineering

Cited by 20 publications

References 75 publications

Biomineralization of Collagen-Based Materials for Hard Tissue Repair

Biomineralization of Collagen-Based Materials for Hard Tissue Repair

Tailoring the growth and proliferation of human dermal fibroblasts by DNA‐based polymer films for skin regeneration

DNA‐Based Materials Inspired by Natural Extracellular DNA

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