Mineralized nodule formation by cultures of human dental pulp-derived fibroblasts

Tsukamoto, Yoshihiko; Fukutani, Sachiko; Shin‐ike, Tsutomu; Kubota, Takao; Sato, Sunao; Suzuki, Yuzuru; Mori, Masakazu

doi:10.1016/0003-9969(92)90037-9

Cited by 141 publications

(104 citation statements)

References 34 publications

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“…Previous studies have looked at the odontogenic potential of adult human dental pulp by using a number of organ, explant, and cell-culture methods and noted the ability of such cultures to mineralize, at least in part (4,19,20,31). Our data also demonstrates the potential of DPSCs to form calcified deposits in vitro, as do BMSCs (5,6,32).…”

Section: Discussionsupporting

confidence: 61%

“…Other growth factors thought to regulate the proliferation and differentiation of odontoblast precursors include basic fibroblast growth factor, platelet-derived growth factor, epidermal growth factor, insulinlike growth factor I tumor necrosis factor-␣, and IL-␤1 (16)(17)(18), all of which influence osteoblastic cells as well. Furthermore, odontoblasts and osteoblasts express similar mineralized matrix proteins, such as dentin matrix protein 1, fibronectin, collagen type I, alkaline phosphatase, osteonectin, osteopontin, bone sialoprotein, and osteocalcin (15,16,(19)(20)(21). Two notable exceptions are the odontoblast-specific gene products, dentin sialoprotein (22) and dentin phosphoprotein (23), encoded by a single gene known as DSPP (24).…”

Section: Discussionmentioning

confidence: 99%

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Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo

Gronthos

Mankani

Brahim

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

4,106

112

3,866

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Dentinal repair in the postnatal organism occurs through the activity of specialized cells, odontoblasts, that are thought to be maintained by an as yet undefined precursor population associated with pulp tissue. In this study, we isolated a clonogenic, rapidly proliferative population of cells from adult human dental pulp. These DPSCs were then compared with human bone marrow stromal cells (BMSCs), known precursors of osteoblasts. Although they share a similar immunophenotype in vitro, functional studies showed that DPSCs produced only sporadic, but densely calcified nodules, and did not form adipocytes, whereas BMSCs routinely calcified throughout the adherent cell layer with clusters of lipidladen adipocytes. When DPSCs were transplanted into immunocompromised mice, they generated a dentin-like structure lined with human odontoblast-like cells that surrounded a pulp-like interstitial tissue. In contrast, BMSCs formed lamellar bone containing osteocytes and surface-lining osteoblasts, surrounding a fibrous vascular tissue with active hematopoiesis and adipocytes. This study isolates postnatal human DPSCs that have the ability to form a dentin͞pulp-like complex.odontoblast ͉ dentin ͉ in vivo transplantation

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo

Gronthos

Mankani

Brahim

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

4,106

112

3,866

View full text Add to dashboard Cite

show abstract

“…Primary human dental pulp cells were isolated from explanted healthy pulp of impacted third molars, following an established protocol (42,43). The cells were cultured in a growth medium containing Dulbecco's modified Eagle's medium (Gibco) with 10% fetal bovine serum, 1% L-glutamine, 10,000 IU/ml penicillin G, 100,000 mg/ml streptomycin sulfate, and 25 mg/ml amphotericin B at 37˚C, 5% CO 2 condition.…”

Section: Methodsmentioning

confidence: 99%

miRNA expression profiling identifies DSPP regulators in cultured dental pulp cells

Huang

Xu²,

Gao³

et al. 2011

Int J Mol Med

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Abstract. Dentin sialophosphoprotein (DSPP), an important marker of odontoblast differentiation, is a prerequisite for tooth development and mineralization; however, the molecular mechanisms of both temporal and spatial regulation remain unknown. MicroRNAs (miRNAs) provide an additional level of control beyond that of transcription factors, which regulate post-transcriptional control of gene expression. The present study was designed to provide a first attempt at an in-depth analysis of dental pulp cells at various odontoblastic differentiation stages to obtain miRNA differential expression patterns, and to determine the contribution of miRNAs in the expression of DSPP during odontoblast differentiation. Dual luciferase reporter assays and qRT-PCR were used to validate miRNAs identified from bioinformatic analyses to determine whether they were able to regulate the DSPP gene in dental pulp cells cultured in a mineralizing medium. The results presented here suggest that DSPP is regulated post-transcriptionally by mir32, mir885-5p and mir586 during odontoblast differentiation.

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“…19,20 Easy obtained potential with minimum pain & morbidity make human DPSCs as a valuable source of MSCs compared to the ordinary sources, such as bone marrow mesenchymal stem cells 21 . In general, DPSCs have been isolated by either outgrowth method, i.e., migration of stem cells from pulp tissue explants (DPSC-OG) [22][23][24] , and/or by enzymatic digestion (DPSC-ED) 4,6,25 . Previous studies have shown that the isolation method and culture conditions can induce different populations or lineages under in vitro passages 26,27 .…”

Section: Introductionmentioning

confidence: 99%