Miniaturisation of a peptide-based electrochemical protease activity sensor using platinum microelectrodes

Uçar, Ahmet; González‐Fernández, Eva; Staderini, Matteo; Avlonitis, Nicolaos; Murray, Alan F.; Bradley, Mark; Mount, Andrew R.

doi:10.1039/c9an02321f

Cited by 25 publications

(15 citation statements)

References 39 publications

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“…Such miniaturized electrodes allow to perform very local measurements, improve the signal-to-noise ratios, obtain faster response times, and decrease the impact of the iR drop [28]. A wide variety of materials have been used for the manufacture of microelectrodes, but the most used electrode materials are noble metals usually, platinum [32,33] or gold [34][35][36][37][38][39], and carbon materials [40][41][42][43][44][45][46][47][48], even some metal oxides [49,50] or semiconductors [51,52] can also be used.…”

Section: Electrodes and Microelectrodesmentioning

confidence: 99%

Electrochemical Biosensing of Dopamine Neurotransmitter: A Review

2021

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Neurotransmitters are biochemical molecules that transmit a signal from a neuron across the synapse to a target cell, thus being essential to the function of the central and peripheral nervous system. Dopamine is one of the most important catecholamine neurotransmitters since it is involved in many functions of the human central nervous system, including motor control, reward, or reinforcement. It is of utmost importance to quantify the amount of dopamine since abnormal levels can cause a variety of medical and behavioral problems. For instance, Parkinson’s disease is partially caused by the death of dopamine-secreting neurons. To date, various methods have been developed to measure dopamine levels, and electrochemical biosensing seems to be the most viable due to its robustness, selectivity, sensitivity, and the possibility to achieve real-time measurements. Even if the electrochemical detection is not facile due to the presence of electroactive interfering species with similar redox potentials in real biological samples, numerous strategies have been employed to resolve this issue. The objective of this paper is to review the materials (metals and metal oxides, carbon materials, polymers) that are frequently used for the electrochemical biosensing of dopamine and point out their respective advantages and drawbacks. Different types of dopamine biosensors, including (micro)electrodes, biosensing platforms, or field-effect transistors, are also described.

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Section: Electrodes and Microelectrodesmentioning

confidence: 99%

Electrochemical Biosensing of Dopamine Neurotransmitter: A Review

2021

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“…An alternative to nanostructured macroscopic electrodes could be microelectrodes [90]. Pt-based microelectrodes of 25 µm diameter were recently applied by Mount's group for the detection of trypsin in a peptide-based biosensor, demonstrating their applicability for the determination of clinically relevant concentrations of proteases [17].…”

Section: Peptides As Substrate and Signal Development Agents In Catalytic Biosensorsmentioning

confidence: 99%

“…Nonelectroactive peptides can be successfully combined with impedance/voltammetric transducers [14,15]. Furthermore, peptides can be easily labelled with redox-active probes, such as ferrocene (Fc) or methylene blue [16][17][18] for signal-on/signal-off detection. Selected applications in clinical diagnostics, in which these kinds of detection strategies are exploited, are discussed in the following sections.…”

Section: Introductionmentioning

confidence: 99%

The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

et al. 2021

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Peptides represent a promising class of biorecognition elements that can be coupled to electrochemical transducers. The benefits lie mainly in their stability and selectivity toward a target analyte. Furthermore, they can be synthesized rather easily and modified with specific functional groups, thus making them suitable for the development of novel architectures for biosensing platforms, as well as alternative labelling tools. Peptides have also been proposed as antibiofouling agents. Indeed, biofouling caused by the accumulation of biomolecules on electrode surfaces is one of the major issues and challenges to be addressed in the practical application of electrochemical biosensors. In this review, we summarise trends from the last three years in the design and development of electrochemical biosensors using synthetic peptides. The different roles of peptides in the design of electrochemical biosensors are described. The main procedures of selection and synthesis are discussed. Selected applications in clinical diagnostics are also described.

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“…1), creating a localised drop in pH at the electrode surface below the pK a of the dipeptide gelator. 36,37 Therefore, gelation can be achieved exclusively at the electrode surface, leaving the pH of the bulk solution unaffected.…”

Section: Introductionmentioning

confidence: 99%

Electrofabrication of large volume di- and tripeptide hydrogels via hydroquinone oxidation

et al. 2022

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Miniaturisation of a peptide-based electrochemical protease activity sensor using platinum microelectrodes

Abstract: A miniaturised sensor displayed efficient analytical performance with differences in kinetics from the macroelectrodes, attributed to the structural differences in probe accessibility.

Cited by 25 publications

References 39 publications

Electrochemical Biosensing of Dopamine Neurotransmitter: A Review

Electrochemical Biosensing of Dopamine Neurotransmitter: A Review

The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

Electrofabrication of large volume di- and tripeptide hydrogels via hydroquinone oxidation

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