Miniaturized Biosensors to Preserve and Monitor Cultural Heritage: from Medical to Conservation Diagnosis

Sciutto, Giorgia; Zangheri, Martina; Anfossi, Laura; Guardigli, Massimo; Prati, Silvia; Mirasoli, Mara; Nardo, Fabio Di; Baggiani, Claudio; Mazzeo, Rocco; Roda, Aldo

doi:10.1002/ange.201713298

Cited by 14 publications

(6 citation statements)

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“…To enable easy handling of sample and reagents, including the addition of the two-component CL substrate for HRP, microfluidic cartridges have recently been proposed for CL-LFIA. The cartridges contain the reagents required for the analysis and deliver them to the LFIA strip in the appropriate time sequence [ 63 , 68 ]. In this context, Zangheri et al, also proposed a portable CL-LFIA biosensor that was successfully used aboard the International Space Station (ISS) by the astronaut Paolo Nespoli (VITA mission, July–December 2017) to monitor salivary cortisol, a stress marker ( Figure 3 ) [ 69 ].…”

Section: Enzyme-labeled Conjugates In Lfiamentioning

confidence: 99%

Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

Calabria

Calabretta

Zangheri

et al. 2021

Sensors

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Paper-based lateral-flow immunoassays (LFIAs) have achieved considerable commercial success and their impact in diagnostics is continuously growing. LFIA results are often obtained by visualizing by the naked eye color changes in given areas, providing a qualitative information about the presence/absence of the target analyte in the sample. However, this platform has the potential to provide ultrasensitive quantitative analysis for several applications. Indeed, LFIA is based on well-established immunological techniques, which have known in the last year great advances due to the combination of highly sensitive tracers, innovative signal amplification strategies and last-generation instrumental detectors. All these available progresses can be applied also to the LFIA platform by adapting them to a portable and miniaturized format. This possibility opens countless strategies for definitively turning the LFIA technique into an ultrasensitive quantitative method. Among the different proposals for achieving this goal, the use of enzyme-based immunoassay is very well known and widespread for routine analysis and it can represent a valid approach for improving LFIA performances. Several examples have been recently reported in literature exploiting enzymes properties and features for obtaining significative advances in this field. In this review, we aim to provide a critical overview of the recent progresses in highly sensitive LFIA detection technologies, involving the exploitation of enzyme-based amplification strategies. The features and applications of the technologies, along with future developments and challenges, are also discussed.

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Section: Enzyme-labeled Conjugates In Lfiamentioning

confidence: 99%

Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

Calabria

Calabretta

Zangheri

et al. 2021

Sensors

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“…This biosensor was successfully used by the Italian astronaut Paolo Nespoli during the VITA mission (July–December 2017), thus demonstrating the possibility of performing sensitive CL-LFIA analyses directly onboard the International Space Station even in microgravity conditions [ 73 ]. CL-LFIA portable devices were also developed to simultaneously detect two proteins (collagen and ovalbumin) for diagnostic campaigns on paintings [ 76 ]. Since the immunological method required a multistep analytical protocol with sequential additions of sample and reagents, a disposable analytical cartridge was developed to streamline the procedure.…”

Section: Progress In Reagents Storage and Self-contained Devices For Poc Applicationmentioning

confidence: 99%

“…Recently, several detectors combining adequate sensitivity with portability have been proposed in order to overcome the main limitation of the relatively weak CL signal and to allow the development of ultrasensitive POCT assays [ 33 ]. For example, CL-based biosensors were implemented with a new generation of (thermally cooled) back-illuminated (BI) CCD integrated with LFIA technologies [ 73 , 75 , 76 , 80 ]. In these examples, the CL measures were conducted in the “contact imaging” approach which consists in placing the LFIA strip directly in contact with CCD sensors through a fiber optic faceplate in order to maximize the photon collection efficiency [ 81 ].…”

Section: Light Detection Technologies Integrated With CL Paper-based Immunoassay Devicesmentioning

confidence: 99%

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Paper-Based Immunosensors with Bio-Chemiluminescence Detection

Calabretta

Zangheri

Calabria

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Since the introduction of paper-based analytical devices as potential diagnostic platforms a few decades ago, huge efforts have been made in this field to develop systems suitable for meeting the requirements for the point-of-care (POC) approach. Considerable progress has been achieved in the adaptation of existing analysis methods to a paper-based format, especially considering the chemiluminescent (CL)-immunoassays-based techniques. The implementation of biospecific assays with CL detection and paper-based technology represents an ideal solution for the development of portable analytical devices for on-site applications, since the peculiarities of these features create a unique combination for fitting the POC purposes. Despite this, the scientific production is not paralleled by the diffusion of such devices into everyday life. This review aims to highlight the open issues that are responsible for this discrepancy and to find the aspects that require a focused and targeted research to make these methods really applicable in routine analysis.

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“…47 A recently developed lateral flow immunoassay (LFI) demonstrated the possibility to detect the presence of two proteins (ovalbumin and collagen) in artworks at the same time, based on the use of disposable cartridges and a chemiluminescent detection system. 48 The extraordinary positive results achieved by means of SERS make it an optimum candidate as a substitute detection technique in such an approach. Indeed, a SERS substrate immunochemically functionalized and labeled with a Raman-probe could represents a promising biosensor to be employed on site, taking advantage of the strong Raman enhancement produced by anisotropic Ag nanostars, 33 able to create a larger number of SERS hot spots than spherical NPs.…”

Section: Sers Sensors For the Detection Of Protein-based Bindersmentioning

confidence: 99%

Review—Electrochemical and SERS Sensors for Cultural Heritage Diagnostics and Conservation: Recent Advances and Prospects

Zalaffi

Karimian

Ugo

2020

J. Electrochem. Soc.

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This review presents the last advances concerning the application to cultural heritage of analytical sensors based on surface enhanced Raman scattering (SERS) and electrochemical transduction. Analytical diagnostics on a work of art is indeed fundamental for dating, restoration and conservation purposes and analytical sensors can provide reliable information at a competitive cost, being usable by non-specialized personal in raw samples and therefore suitable for on-site analysis. After a brief overview on the problem and specificities related to the analysis of works of art, principles and application of SERS for sensing pigments and dyes in paintings is reviewed. Concerning electrochemical sensors, the use of the so-called voltammetry of microparticles is introduced and discussed. For the analysis of macromolecules of biological origin such as protein binders in ancient paintings, the development of immunosensors which exploit SERS or electrochemical detection is presented together with the discussion of the role that advanced nanomaterials plays in improving the functioning of such devices. Finally, prospects and limits derived from the application of the above sensing devices for diagnostics and monitoring of cultural heritage are discussed.

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Miniaturized Biosensors to Preserve and Monitor Cultural Heritage: from Medical to Conservation Diagnosis

Abstract: Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

Cited by 14 publications

References 25 publications

Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

Paper-Based Immunosensors with Bio-Chemiluminescence Detection

Review—Electrochemical and SERS Sensors for Cultural Heritage Diagnostics and Conservation: Recent Advances and Prospects

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