Miniaturized sensor for electroanalytical and electrochemiluminescent detection of pathogens enabled through laser-induced graphene electrodes embedded in microfluidic channels

Gerstl, Florian; Pongkitdachoti, Uma; Unob, Fuangfa; Baeumner, Antje J.

doi:10.1039/d2lc00593j

Cited by 11 publications

(9 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The obtained LOD is similar to those generated in significantly more sophisticated systems (Table ). Microfluidic liposome-based systems, in which active pumping of the solution and more complex assay conditions are required, showed a comparable, but better LOD . The systems that used liposomes as labels with ECL as detection strategy show a better LOD, which is expected since the redox molecules can be electrochemically regenerated and lead to a redox cycling amplification process .…”

Section: Resultsmentioning

confidence: 99%

“…Microfluidic liposome-based systems, in which active pumping of the solution and more complex assay conditions are required, showed a comparable, but better LOD. 28 The systems that used liposomes as labels with ECL as detection strategy show a better LOD, which is expected since the redox molecules can be electrochemically regenerated and lead to a redox cycling amplification process. 31 However, compared to other, more similar systems, 29,32 the LCNF-LFA has a better LOD, except when more sensitive detection strategies such as SWASV 33 are used.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Flow-Through Carbon Nanofiber-Based Transducer for Inline Electrochemical Detection in Paper-Based Analytical Devices

Perju,

Holzhausen,

Lauerer

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Point-of-care (POC) devices are rapid, simple, portable, inexpensive, and convenient, but typically they only deliver qualitative results when used in the form of a lateral flow assay (LFA). Electrochemical detection could improve their sensitivity and ensure quantitative detection; however, a breakthrough in material-based technology is needed. We demonstrate a new concept in which electrodes are directly embedded within the lateral flow, enabling flow-through and hence interaction with the entire sample. This is accomplished through laser-induced carbon nanofibers (LCNFs) made by electrospinning Matrimid into nanofiber mats with subsequent pyrolyzing of electrode structures through a CO 2 laser. Their highly porous 3D structure and superior graphene-like electrochemical properties are ideally suited for flow-through electrochemical LFA (EC-LFA), where the LCNFs are simply added in line with the other membranes. After optimization of the setup, biological binding assays typical for LFA diagnostics were successfully implemented, enabling the highly sensitive and quantitative detection of 137 pM DNA target sequences of a pathogenic organism that rivals the performance of pump-controlled microfluidic bioassays. This demonstrates that LCNF-based transducers can transform paper-based diagnostic tests to enable precise, quantitative analysis without reliance on cost-intensive read-out systems.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Flow-Through Carbon Nanofiber-Based Transducer for Inline Electrochemical Detection in Paper-Based Analytical Devices

Perju,

Holzhausen,

Lauerer

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…Very recently, researchers have begun to explore the direct conversion of insulating polymers, such as polyimide (Kapton) [18] and a variety of commercial polymers, [19] to conductive graphenic materials using CO 2 laser cutters/engravers. In particular, laser-pyrolized polyimide substrates have been used in a number of biosensing applications, including small molecule detection, [20][21][22] protein-based immunoassays, [23,24] and DNA [25] or miRNA [24] recognition. Laser-induced pyrolysis of cellulose is a comparatively nascent field and to date has been exploited for artistic depictions, [26] simple electric circuits, [27] mechanical sensing, [28,29] and more recently small molecule detection.…”

Section: Introductionmentioning

confidence: 99%

Paper‐Based Laser‐Pyrolyzed Electrofluidics: An Electrochemical Platform for Capillary‐Driven Diagnostic Bioassays

et al. 2023

View full text Add to dashboard Cite

Microfluidic paper-based analytical devices (μPADs) are indispensable tools for disease diagnostics. The integration of electronic components into μPADs enables new device functionalities and facilitates the development of complex quantitative assays. Unfortunately, current electrode fabrication methods often hinder capillary flow, considerably restricting μPAD design architectures. Here, laser-induced graphenization is presented as an approach to fabricate porous electrodes embedded into cellulose paper. The resulting electrodes not only have high conductivity and electrochemical activity, but also retain wetting properties for capillary transport. Paper-based electrofluidics, including a lateral flow device for injection analysis of alkaline phosphatase in serum and a vertical flow device for quantitative detection of HPV16 with a CRISPR-based assay are demonstrated. It is expected that this platform will streamline the development of diagnostic devices that combine the operational simplicity of colorimetric lateral flow tests with the added benefits and possibilities offered by electronic signaling.

show abstract

“…Very recently, researchers have begun to explore the direct conversion of insulating polymers, such as polyimide (Kapton) (18) and a variety of commercial polymers (19), to conductive graphenic materials using CO2 laser cutters/engravers. In particular, laser-pyrolized polyimide substrates have been used in a number of biosensing applications, including small molecule detection (20,21), protein-based immunoassays (22,23), and DNA (24) or miRNA (23) recognition. Laser-induced pyrolysis of cellulose is a comparatively nascent field and to date has been exploited for artistic depictions (25), simple electric circuits (26), mechanical sensing (27,28), and more recently small molecule detection (29)(30)(31)(32).…”

Section: Introductionmentioning

confidence: 99%

Paper-based laser-pyrolyzed electrofluidics: an electrochemical platform for capillary-driven diagnostic bioassays

Bezinge

Lesinski

Suea‐Ngam

et al. 2023

Preprint

View full text Add to dashboard Cite

Microfluidic paper-based analytical devices (uPADs) are indispensable tools for disease diagnostics. The integration of electronic components into uPADs enables new device functionalities and facilitates the development of complex quantitative assays. Unfortunately, current electrode fabrication methods often hinder capillary flow, considerably restricting uPAD design architectures. Here, we present laser-induced graphenization as an approach to fabricate porous electrodes embedded into cellulose paper. The resulting electrodes not only have high conductivity and electrochemical activity, but also retain wetting properties for capillary transport. We demonstrate paper-based electrofluidics, including (i) a lateral flow device for injection analysis of alkaline phosphatase in serum and (ii) a vertical flow device for quantitative detection of HPV16 with a CRISPR-based assay. We expect that this platform will streamline the development of diagnostic devices that combine the operational simplicity of colorimetric lateral flow tests with the added benefits and possibilities offered by electronic signaling.

show abstract

Miniaturized sensor for electroanalytical and electrochemiluminescent detection of pathogens enabled through laser-induced graphene electrodes embedded in microfluidic channels

Abstract: High performance, laser-induced graphene (LIG) electrodes were integrated into adhesive tape-based microfluidic channels to realize both electrochemical (EC) and electrochemiluminescent (ECL) detection approaches. This provides strategies for low limits of...

Cited by 11 publications

References 43 publications

Flow-Through Carbon Nanofiber-Based Transducer for Inline Electrochemical Detection in Paper-Based Analytical Devices

Flow-Through Carbon Nanofiber-Based Transducer for Inline Electrochemical Detection in Paper-Based Analytical Devices

Paper‐Based Laser‐Pyrolyzed Electrofluidics: An Electrochemical Platform for Capillary‐Driven Diagnostic Bioassays

Paper-based laser-pyrolyzed electrofluidics: an electrochemical platform for capillary-driven diagnostic bioassays

Contact Info

Product

Resources

About