Minimal Invasive Cystometry and Intra-Abdominal Pressure Assessments in Rodents: A Novel Animal Study

Abstract

BackgroundThe abdominal straining pattern can act as a novel parameter for improving the prediction of bladder outlet obstruction (BOO). To preserve detrusor function in the early stage of urinary system impairment, such as BOO, we establish a novel method for cystometry and Intra-abdominal pressure (IAP) assessments in rodents without cystostomy.Material/MethodsTwenty mice and rats were divided into three groups (control, sham-operated and BOO group) respectively. The cystometry and IAP assessments were measured by the pediatric venous indwelling sheath and coronary dilatation catheter connected to Laborie urodynamic system on postoperative day 7. Data was collected simultaneously through urethra and rectum in each group. In addition, bladder histology was assessed to confirm BOO.ResultsThe novel method can collect the urodynamic parameters successfully, including the BLPP, IAP, MBC, etc. IAP was elevated in BOO rats, but no significantly difference was found between the sham-operated rats and the control rats. The hypertrophy of detrusor muscle in bladder section was observed by Masson trichrome staining in BOO group compared with other groups.ConclusionsOur novel method based on innovative research implement for cystometry and IAP assessments in rodents is a reliable and replicable approach for evaluating the lower urinary tract function. Especially it provides detailed information to evaluate lower urinary tract structures and function in the early stage of BOO.

Suture causing urethral meatus stricture: A novel animal model of partial bladder outlet obstruction

“…We used polypropylene suture to create the UMS model to ensure scientific truthfulness because this suture is primarily used in cardiovascular surgery, exhibits low tissue reactivity, and improved biostability properties, and is not corroded by urine . In addition, different saline infusion rates have been described for mouse urodynamics, ranging from 0.6 to 3 mL/h . A moderate rate of infusion (2 mL/h) was used in our studies because this rate simulates the process of urine formation.…”

METTL3‐dependent m6A modification mediates bladder remodeling after partial bladder outlet obstruction through CCN2 activation

“…The cystometry and pressure-flow parameters were acquired as described previously. 24,25 Briefly, cystometric investigations were conducted 4 weeks postsurgery in urethane-anesthetized (1.2 g/kg, i.p.) rats.…”