Minimally invasive skin tape strip RNA sequencing identifies novel characteristics of the type 2–high atopic dermatitis disease endotype

Dyjack, Nathan; Goleva, Elena; Rios, Cydney; Kim, Byung Eui; Bin, Lianghua; Taylor, Patricia A.; Bronchick, Caroline; Hall, Clifton F.; Richers, Brittany; Seibold, Max A.; Leung, Donald Y.M.

doi:10.1016/j.jaci.2017.10.046

Cited by 95 publications

(179 citation statements)

References 51 publications

Supporting

Mentioning

170

Contrasting

Unclassified

Order By: Relevance

“…Therefore, studies that compare findings of cells from tape strips with those obtained from skin biopsies are needed. In line with this, a study from 2018 compared the RNA transcripts from cells obtained by tape stripping with cells from skin biopsies in patients with atopic dermatitis, and showed that the expression profiles from cells obtained by tape stripping were different from those found in skin biopsies, and can be used to identify novel endotypes of atopic dermatitis . The methodology, however, needs to be further developed.…”

Section: Discussionmentioning

confidence: 97%

“…In line with this, a study from 2018 compared the RNA transcripts from cells obtained by tape stripping with cells from skin biopsies in patients with atopic dermatitis, and showed that the expression profiles from cells obtained by tape stripping were different from those found in skin biopsies, and can be used to identify novel endotypes of atopic dermatitis. 8 The methodology, however, needs to be further developed. Moreover, the large interindividual variation that we observed Black dots represent the number of tape strippings until complete removal of the stratum corneum (SC).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Skin tape stripping: Which layers of the epidermis are removed?

et al. 2019

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Skin tape stripping: Which layers of the epidermis are removed?

et al. 2019

View full text Add to dashboard Cite

“…In these studies, skin emollients reduced AD occurrence by approximately 50%. Transcriptomic studies found that nonlesional, normal-appearing skin of AD patients is distinct from the skin of healthy control subjects, with evidence of type 2 inflammatory responses and reduced skin barrier function in nonlesional AD (64,140,154,204). It would be interesting to determine whether addition of intermittent proactive topical antiinflammatory therapies, including low-potency topical corticosteroids (205), which are known to reduce eczema relapse down to 3 months of age, further reduces AD and FA occurrence.…”

Section: Strategies To Improve the Epithelial Barrier And Prevent Allmentioning

confidence: 99%

“…In addition to TEWL measurements at the skin surface, TEWL measurements can be combined with STS to measure skin barrier integrity. With STS, the uppermost layers of the skin are peeled away using adhesive discs (154,204). Skin with compromised skin barrier already present in nonlesional, normal-appearing AD skin (64,154,204), and are further aggravated in AD lesional skin.…”

Section: Noninvasive Approaches For Assessing Skin Functionmentioning

confidence: 99%

Epithelial barrier repair and prevention of allergy

Goleva¹,

Berdyshev

Leung

2019

Journal of Clinical Investigation

Self Cite

176

173

View full text Add to dashboard Cite

Allergic diseases such as atopic dermatitis (AD), food allergy (FA), asthma, and allergic rhinitis affect more than 30% of the population (1-3). These diseases have in common a dysfunctional epithelial barrier, resulting in the penetration of allergens and microbes, accompanied by the release of epithelial-derived cytokines (e.g., thymic stromal lymphopoietin [TSLP], IL-25, IL-33), which drive type 2 immune responses. Although other immune pathways can modify the course of illness, cytokines including IL-4, IL-13, IL-31, TSLP, and IL-33 play a key role in allergic diseases (4-8), eliciting local tissue injury and repair (5,(9)(10)(11)(12)(13)(14).AD is the most prevalent chronic inflammatory skin disease (3,(15)(16)(17)(18)(19). In a subset of allergic patients, it is thought that ADrelated skin epithelial dysfunction contributes to the atopic march, which starts with AD and often leads directly to FA (20-23). The link from AD to respiratory allergy is more controversial; however, atopic march progression is facilitated in patients who develop IgE to both food and inhalant allergens (24).In this Review, we describe formation of the skin barrier, review the link between altered skin barrier formation and AD, discuss evidence for epithelial barrier dysfunction in other allergic diseases, and explore epithelial barrier intervention/repair strategies with the goal of preventing AD and the atopic march. The cornified envelope and keratinocyte differentiation in the skinThe skin's barrier function primarily depends on the outermost epidermal layer, stratum corneum (SC). To form SC, keratinocytes pass through a tightly regulated differentiation program and sequentially form stratum basale (SB), stratum spinosum (SS), stratum granulosum (SG), and SC layers of the skin (Figure 1). In human skin, keratinocytes irreversibly exit the cell cycle after mitosis in the basal layer and differentiate progressively across the epidermis toward the SC. Each layer is defined by its expression of characteristic morphological and biochemical features indicating its state of differentiation (25-27). The keratins KRT5 and KRT14 are predominantly expressed by basal keratinocytes. The markers of early differentiation KRT1 and KRT10 are made in SS. The cornified envelope precursors involucrin (IVL) and transglutaminase-1 (TGM1) are also present in SS. Late differentiation markers including the cornified envelope protein loricrin (LOR) and the precursor of the keratin cross-linking protein filaggrin (FLG) are expressed in SG keratinocytes. TGM1 cross-links IVL, LOR, and other structural proteins to form the cornified envelope.Calcium forms a steep gradient across the human epidermis, increasing from the SB (5 μM) to the outer SG (>20 μM) (27, 28). Importantly, there is cell-to-cell heterogeneity in average Ca 2+ concentrations in the epidermis (28). Formation of the Ca 2+ gradient coincides with key developmental milestones of skin barrier formation and differentiation into the SC.Primary human keratinocyte cultures are an excellent model for...

show abstract

“…(B) Top‐50 down DEG ( LS vs NL ). The three studies included are Tape: Tape‐stripped skin (not in GEO ) ; LCM : GSE 120721 ; Shave: GSE 60709 . For the genes that are up in LS / NL epidermis, the overlap in DEG between LCM and epidermal shave is 50% (25 out of 50 genes).…”

Section: From Disease Understanding To Biomarkers Endotypes and Tarmentioning

confidence: 99%

Personalized medicine—concepts, technologies, and applications in inflammatory skin diseases

Litman

2019

APMIS

View full text Add to dashboard Cite

show abstract

Minimally invasive skin tape strip RNA sequencing identifies novel characteristics of the type 2–high atopic dermatitis disease endotype

Abstract: Minimally invasive expression profiling of nonlesional skin reveals stratification in AD molecular pathology by type 2 inflammation that correlates with disease severity.

Cited by 95 publications

References 51 publications

Skin tape stripping: Which layers of the epidermis are removed?

Skin tape stripping: Which layers of the epidermis are removed?

Epithelial barrier repair and prevention of allergy

Personalized medicine—concepts, technologies, and applications in inflammatory skin diseases

Contact Info

Product

Resources

About