Minimization of protein adsorption on poly(vinylidene fluoride)

Ademović, Zahida; Klee, Doris; Kingshott, Peter; Kaufmann, R.; Höcker, Hartwig

doi:10.1016/s1389-0344(02)00020-5

Cited by 41 publications

(38 citation statements)

References 13 publications

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“…Poly(ethylene glycol) (PEG) is widely used as coating material due to its known biocompatibility and potential to minimize unspecific protein adsorption . It has been shown that high grafting densities resulting in high surface coverage of the polymer is a determining factor for protein resistance of PEG . Furthermore, it has been predicted that branched polymer architectures should be superior compared to linear ones for the prevention of unspecific protein adsorption .…”

Section: Introductionmentioning

confidence: 99%

“…[45][46][47][48] It has been shown that high grafting densities resulting in high surface coverage of the polymer is a determining factor for protein resistance of PEG. 49,50 Furthermore, it has been predicted that branched polymer architectures should be superior compared to linear ones for the prevention of unspecific protein adsorption. 51,52 Earlier, we investigated the protein repelling properties of linear and sPEG using isocyanate-terminated, star-shaped poly(ethylene-co-propylene) (NCO-sP(EO-stat-PO)) statistical copolymer showing the great potential of the NCO-sP(EO-stat-PO) prepolymer for coating applications.…”

Section: Introductionmentioning

confidence: 99%

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Dexamethasone released from cochlear implant coatings combined with a protein repellent hydrogel layer inhibits fibroblast proliferation

Wrzeszcz

Dittrich

Haamann

et al. 2013

J Biomedical Materials Res

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The insertion of cochlear implants into the inner ear often causes inflammation and fibrosis inside the scala tympani and thus growth of fibrous tissue on the implant surface. This deposition leads to the loss of function in both electrical and laser-based implants. The design of this study was to realize fibroblast growth inhibition by dexamethasone (Dex) released from the base material of the implant [polydimethylsiloxane (PDMS)]. To prevent cell and protein adhesion, the PDMS was coated with a hydrogel layer [star-shaped polyethylene glycol prepolymer (sPEG)]. Drug release rates were studied over 3 months, and surface characterization was performed. It was observed that the hydrogel slightly smoothened the surface roughened by the Dex crystals. The hydrogel coating reduced and prolonged the release of the drug over several months. Unmodified, sPEG-coated, Dex-loaded, and Dex/sPEG-equipped PDMS filaments were cocultivated in vitro with fluorescent fibroblasts, analyzed by fluorescent microscopy, and quantified by cell counting. Compared to the unmodified PDMS, cell growth on all modified filaments was averagely 95% ±standard deviation (SD) less, while cell growth on the bottom of the culture dishes containing Dex-loaded filaments was reduced by 70% ±SD. Both, Dex and sPEG prevented direct cell growth on the filament surfaces, while drug delivery was maintained for the duration of several months.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dexamethasone released from cochlear implant coatings combined with a protein repellent hydrogel layer inhibits fibroblast proliferation

Wrzeszcz

Dittrich

Haamann

et al. 2013

J Biomedical Materials Res

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“…Because of the fact that PVDF does not possess functional groups that allow for surface modification and its intrinsic inertness, the surface must be activated for chemical binding by a plasma treatment. [28][29][30][31][32][33] In the presented work, two-and three-dimensional PVDF samples have been treated by low-pressure MW-induced ammonia-plasma to induce the creation of amine groups on the PVDF surface [ Fig. 1(a)].…”

Section: Coating Of Two-and Three-dimensional Pvdf Substrates For Chamentioning

confidence: 99%

Bio‐functionalized star PEG‐coated PVDF surfaces for cytocompatibility‐improved implant components

Heuts¹,

Salber²,

Goldyn³

et al. 2009

J Biomedical Materials Res

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Unmodified and GRGDS peptide-modified six arm PEG star based hydrogels (Star PEG) have been applied as a multifunctional, easy to handle coating system for textile polyvinylidene fluoride (PVDF) structures, which prevent unspecific protein and cell adsorption and control-specific cell adhesion. The reactive isocyanate-terminated Star PEG has been successfully applied to ammonia-plasma treated two- and three-dimensional PVDF surfaces. Easy modification of the surface hydrogel by mixing in of GRGDS peptide during the coating step or subsequent coupling of GRGDS was determined by TOF-SIMS. Unmodified and GRGDS-functionalized hydrogel surfaces show distinct protein repellency, as demonstrated by fluorescence microscopy after incubation with fluorescent labeled proteins and Surface MALDI-TOF-Mass Spectroscopy. Cell culture experiments with primary human dermal fibroblasts, primary fetal rat fibroblasts, and human osteoblasts on GRGDS and/or KRSR Star PEG-modified two- and three-dimensional substrates show advancement in cell adhesion and proliferation compared with untreated PVDF surfaces, whereas pure star PEG-coated surfaces show no cell adhesion. The combination of protein and cell repellent properties with specific biofunctionality and easy application of the coatings will enable their application for 3D-scaffolds.

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“…Therefore, synthetic polymers require continuous improvement in terms of hemocompatibility, with enhanced suppression of protein adsorption and clot formation. To improve the anti-fouling property of hemopurification membranes, researchers have developed various strategies that may control hydrophilia in hydrophobic synthetic polymer materials at the structural and surface-active levels (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic monomers suppress the adsorption of plasma protein onto a poly(vinylidene fluoride) membrane

Takahashi

Higuchi²

2009

Mol Med Rep

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Abstract. dialysis is the single most important therapy for chronic kidney disease. However, protein adsorption onto hemodialysis membranes promotes clot formation. The aim of the present study was to develop a surface-modified membrane which suppresses protein adsorption. using plasma polymerization, hydrophilic n-vinyl pyrrolidone (VP) and acryloyl morpholine (acMo) were polymerized on hydrophobic poly(vinylidene fluoride) (PVDF) surfaces. PVDF is employed as a biomaterial owing to its extraordinary durability. These membranes were then characterized using static contact angle measurement, ATR-FTIR spectra and polyacrylamide gel electrophoresis. The surface-modified PVDF membranes showed a transition of the surface from relatively hydrophobic to hydrophilic. less proteins were adsorbed on the VP and ACMO grafted PVDF surfaces than on the unmodified membrane and the commercially available hemodialysis membrane. In conclusion, VP and ACMO grafted PVDF membranes have a high potential for the inhibition of protein adsorption in high-performance hemopurification devices.

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Minimization of protein adsorption on poly(vinylidene fluoride)

Cited by 41 publications

References 13 publications

Dexamethasone released from cochlear implant coatings combined with a protein repellent hydrogel layer inhibits fibroblast proliferation

Dexamethasone released from cochlear implant coatings combined with a protein repellent hydrogel layer inhibits fibroblast proliferation

Bio‐functionalized star PEG‐coated PVDF surfaces for cytocompatibility‐improved implant components

Hydrophilic monomers suppress the adsorption of plasma protein onto a poly(vinylidene fluoride) membrane

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