Mining, Isolation and Identification of Siderophore Synthesis Gene from Brevibacillus brevis GZDF3

Abstract

Objective of this paper is to excavate the siderophore synthesis gene from Brevibacillus brevis GZDF3 and verify its type and antibacterial effects. The method is using genome mining technology to analyze the siderophore synthesis genes and the phylogenetic tree of each synthesis gene was constructed separately. Iron free medium was utilized to induce the synthesis of siderophore and CAS liquid detection method was used for qualitative and quantitative analysis on siderophore. The type of siderophore was preliminaries identified by Arnow and its antibacterial effects were analyzed according to the agar punching method. The results show that a siderophore synthesis gene cluster with 83% similarity to Petrobactin was found in Brevibacillus brevis GZDF3 genome. Iron free medium could induce siderophore synthesis and the optimal incubation time cultured in iron free medium was 30 h and 48 h. Antagonistic strain GZDF3 had the capacity to synthesize catechol-type siderophore. Also, GZDF3 had a powerful antibacterial effect on pathogenic fungus Fusarium oxysporum of rotted root on Pinellia ternata. Therefore, Brevibacillus brevis GZDF3 can produce catechol-type siderophore in an iron-deficient culture medium, which was also a main antifungal active substance.

Siderophore Production by Rhizosphere Biological Control Bacteria Brevibacillus brevis GZDF3 of Pinellia ternata and Its Antifungal Effects on Candida albicans

“…The siderophore production was determined by CAS liquid test. The cell growth and siderophore production were measured as a function of fermentation time at 0, 6,12,18,24,30,36,42,48,54, and 60 h. The pH was adjusted to 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, and 10.0, respectively. Fermentation temperature was set from 23°C to 37°C.…”

“…Our previous research reported that B. brevis GZDF3 can produce catechol-type siderophores and has a strong antagonistic effect on F. oxysporum . However, factors that affect the production of siderophores and the antifungal effect against Candida albicans of B. brevis GZDF3 have not yet been reported [ 12 ].…”

Consortium of plant growth-promoting rhizobacteria enhances oilseed rape (Brassica napus L.) growth under normal and saline conditions

“…We found that B14 strain carried entD gene which encodes a product necessary for the synthesis of the iron-chelating and transport siderophore enterobactin (Khan et al 2018), while the genome of B19 contained asbF gene involved in 3,4-dihydroxybenzoic acid synthase that is necessary for petrobactin production (Hotta et al 2010). Previous studies indicated that Brevibacillus brevis showed strong antagonistic effect against F. oxysporum and siderophore synthesis gene cluster with 83 % similarity to petrobactin was found in the genome of this strain (Sheng et al 2018).…”

Physiological properties and genomic insights into the plant growth‐promoting rhizobacterium Brevibacillus laterosporus K75 isolated from maize rhizosphere

“…Furthermore, both KEGG Mapper and antiSMASH analysis of the K75 genome revealed the presence of a gene involved in the synthesis of petrobactin, and in vitro assay showed a strong ability to produce siderophores by B. lateropsorus K75. Sheng et al 47 reported that B. brevis GZDF3 had strong antifungal activity, and a siderophore synthesis gene cluster that was 83% similar to petrobactin was annotated in the genome. Moreover, other biosynthetic gene clusters involved in antimicrobial compounds synthesis were found.…”