Mining metagenomic whole genome sequences revealed subdominant but constant <i>Lactobacillus</i> population in the human gut microbiota

Rossi, Maddalena; Martínez-Martínez, Daniel; Amaretti, Alberto; Ulrici, Alessandro; Raimondi, Stefano; Moya, Andrés

doi:10.1111/1758-2229.12405

Cited by 66 publications

(52 citation statements)

References 47 publications

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“…Taking into account the plausible presence of the same strains over > 6 months, it is possible to hypothesize the occurrence of a promiscuous microbiota able to survive in the plant, despite the strong sanitization procedures, and in the meat. This is consistent with the wide distribution of LAB species in the environment, which goes far beyond the attribution of each species to specific habitats (Rossi et al, 2016).…”

Section: Identification Of the Isolates At The End Of The Shelf Lifesupporting

confidence: 83%

Bacterial community of industrial raw sausage packaged in modified atmosphere throughout the shelf life

Raimondi

Nappi

Sirangelo

et al. 2018

International Journal of Food Microbiology

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Ten lots of industrial raw sausages in modified atmosphere (CO 30%, O 70%), produced in the same plant over 7 months, were analyzed at the day after production (S samples) and at the end of shelf life (E samples), after 12 days storage at 7 °C to simulate thermal abuse. Quality of the products was generally compromised by storage at 7 °C, with only 3 E samples without alterations. During the shelf life, the pH decreased for the accumulation of acetic and lactic acids. A few biogenic amines accumulated, remaining below acceptable limits. The profile of volatile compounds got enriched with alcohols, ketones, and acids (e.g. ethanol, 2,3-butanediol, 2,3-butandione, butanoic acid) originated by bacterial metabolism. Throughout the shelf life, aerobic bacteria increased from 4.7 log to 6.6 log cfu/g, and lactic acid bacteria (LAB) from 3.7 to 8.1 log cfu/g. Staphylococci, enterobacteria, and pseudomonads passed from 3.7, 3.0, and 1.7 to 5.5, 4.8, and 3.0 log cfu/g, respectively. Dominant cultivable LAB, genotyped by RAPD-PCR, belonged to the species Lactobacillus curvatus/graminis and Lactobacillus sakei, with lower amounts of Leuconostoc carnosum and Leuconostoc mesenteroides. Brochothrix thermosphacta was the prevailing species among aerobic bacteria. The same biotypes ascribed to several different species where often found in E samples of diverse batches, suggesting a recurrent contamination from the plant of production. Profiling of 16S rRNA gene evidenced that microbiota of S samples clustered in two main groups where either Firmicutes or Bacteroidetes prevailed, albeit with taxa generally associated to the gastro-intestinal tract of mammals. The microbial diversity was lower in E samples than in S ones. Even though a common profile could not be identified, most E samples clustered together and were dominated by Firmicutes, with Lactobacillaceae and Listeriaceae as the most abundant families (mostly ascribed to Lactobacillus and Brochothrix, respectively). In a sole E sample Proteobacteria (especially Serratia) was the major phylum.

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Section: Identification Of the Isolates At The End Of The Shelf Lifesupporting

confidence: 83%

Bacterial community of industrial raw sausage packaged in modified atmosphere throughout the shelf life

Raimondi

Nappi

Sirangelo

et al. 2018

International Journal of Food Microbiology

Self Cite

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“…Wide diversity in the adhesion properties has been recorded among the putative probiotic bacterial species, including strains of Lactobacillus plantarum , that is one of the most predominant species of the intestinal microbiota . Recently, Mukherjee et al have reported that some L. plantarum strains exhibited superior adhesion ability to ECM proteins as compared with other LAB strains and Staphylococcus aureus .…”

Section: Introductionmentioning

confidence: 99%

Pyruvate dehydrogenase subunit β of Lactobacillus plantarum is a collagen adhesin involved in biofilm formation

Salzillo¹,

Vastano²,

Capri³

et al. 2016

J Basic Microbiol

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Multi-functional surface proteins have been observed in a variety of pathogenic bacteria, where they mediate host cell adhesion and invasion, as well as in commensal bacterial species, were they mediate positive interaction with the host. Among these proteins, some glycolytic enzymes, expressed on the bacterial cell surface, can bind human extracellular matrix components (ECM). A major target for them is collagen, an abundant glycoprotein of connective tissues. We have previously shown that the enolase EnoA1 of Lactobacillus plantarum, one of the most predominant species in the gut microbiota of healthy individuals, is involved in binding with collagen type I (CnI). In this study, we found that PDHB, a component of the pyruvate dehydrogenase complex, contributes to the L. plantarum LM3 adhesion to CnI. By a cellular adhesion assay to immobilized CnI, we show that LM3-B1 cells, carrying a null mutation in the pdhB gene, bind to CnI - coated surfaces less efficiently than wild-type cells. Moreover, we show that the PDHB-CnI interaction requires a native state for PDHB. We also analyzed the ability to develop biofilm in wild-type and mutant strains and we found that the lack of the PDHB on cell surface generates cells partially impaired in biofilm development.

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“…According to Walter [33], Lactobacillus brevis is one of Lactobacillus species commonly detected in human feces. Lactobacilli are still regarded as dominant microorganisms of the humans and animals gut [34][35][36].…”

Section: B Phenotype Characteristics Of Lab Isolated From Civet's DImentioning

confidence: 99%

Phenotypic Identification of Lactic Acid Bacteria From Civet (Paradoxorus Hermaphroditus)

Muzaifa¹,

Hasni²,

Patria³

et al. 2019

Int. J. Adv. Sci. Eng. Inf. Technol.

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Kopi luwak (civet coffee) is widely known as the most expensive coffee in the world. As quality is known, the popularity of kopi luwak also increases, which leads to the increasing demands for this coffee. Kopi luwak has high economic value due to its rarity, unique flavor. and a distinctive production process. An alternative for kopi luwak production is employing an in vitro fermentation to imitate the natural fermentation on the digestive system of civet. Fermentation is usually carried out by indigenous microflora, the existence of the microflora thought to have a role in civet coffee production. A type of bacteria suspected a role in the digestive tract is lactic acid bacteria (LAB). The study aims to identify LAB from the digestive tract of civet. The isolation of LAB was carried out by using MRS agar, and their characterization by phenotypic analysis. The results showed that the characteristic of LAB varied. Based on API analysis, the study successfully identified LAB, which are Pediococcus pentosaceus, Lactococcus lactis spp. lactis, Lactobacillus fructivorans, and Lactobacillus brevis. LAB with protease and pectinase activity might be used as a starter culture for the production of artificial kopi luwak. Hence, a further study related to their technical characteristics must be done to maintain kopi luwak availability.

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Mining metagenomic whole genome sequences revealed subdominant but constant Lactobacillus population in the human gut microbiota

Cited by 66 publications

References 47 publications

Bacterial community of industrial raw sausage packaged in modified atmosphere throughout the shelf life

Bacterial community of industrial raw sausage packaged in modified atmosphere throughout the shelf life

Pyruvate dehydrogenase subunit β of Lactobacillus plantarum is a collagen adhesin involved in biofilm formation

Phenotypic Identification of Lactic Acid Bacteria From Civet (Paradoxorus Hermaphroditus)

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