Mining the global diversity of barley for Fusarium resistance using leaf and spike inoculations

Bedawy, Ismail M.; Dehne, H. W.; Léon, Jens; Naz, Ali Ahmad

doi:10.1007/s10681-017-2103-1

Cited by 8 publications

(5 citation statements)

References 32 publications

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“…While the study identified QTL for reduced FHB and DON, as seen in a previous study, negative associations with agronomic characters were not present, indicating that this germplasm could be useful in breeding programs without the confounding effects related to the heading date or plant height. Bedawy et al (2018) conducted leaf and spike disease assays with a diversity panel comprised 140 genotypes in combination with DArT and iSelect SNP marker assays, identifying QTL for each character on chromosomes 1 H and 5 H, respectively. They also identified positive epistatic interactions contributing to disease reduction.…”

Section: Modern Biotechnologies and How They Facilitate Fhb Researchmentioning

confidence: 99%

Building on a foundation: advances in epidemiology, resistance breeding, and forecasting research for reducing the impact of fusarium head blight in wheat and barley

Fernando

Oghenekaro

Tucker

et al. 2021

Canadian Journal of Plant Pathology

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Section: Modern Biotechnologies and How They Facilitate Fhb Researchmentioning

confidence: 99%

Building on a foundation: advances in epidemiology, resistance breeding, and forecasting research for reducing the impact of fusarium head blight in wheat and barley

Fernando

Oghenekaro

Tucker

et al. 2021

Canadian Journal of Plant Pathology

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“…of the disease in the field caused by the interaction of phenotypic characteristics and environmental conditions (Dahl and Wilson, 2018). Developing in vitro methods that are simple to be conducted and less time consuming aimed to better monitor disease intensity is critical for the control of FHB (Hestbjerg et al, 2002;Kumar et al, 2011;Opoku et al, 2011;Bedawy et al, 2018;Sakr, 2018aSakr, , b, 2019bSakr, , 2020bSakr and Shoaib, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…A small amount of plant material is required for each in vitro assay. However, some studies referenced herein did not investigate correlations with the head blight reaction in adult barley plant (Hestbjerg et al, 2002;Kumar et al, 2011;Opoku et al, 2011;Bedawy et al, 2018). Sakr (2018a) observed a similar range of aggressiveness in FHB isolates recovered from diseased wheat heads on Arabi Aswad (AS) barley and durum wheat (Triticum durum) plants in vitro.…”

Section: Introductionmentioning

confidence: 88%

A simple and reliable assay to quantify host resistance and aggressiveness of the pathogen in the Fusarium head blight-barley pathosystem

Sakr

Al-Attar

2022

APhyt

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A simple and reliable method for quantifying Fusarium head blight (FHB), a widespread disease of barley, would enhance our capacity in identifying resistance sources and highly aggressive isolates. A detached head assay (DHA) was used to reliably assess: (i) resistance of two barley cultivars, Arabi Aswad (AS) and Arabi Abiad (AB) with different susceptibility to FHB and (ii) aggressiveness in a set of 16 fungal isolates of four Fusarium species. The two inoculated cultivars showed different responses in FHB incidence (DI) and severity (DS) using spray and point inoculation on detached barley heads, respectively. On AB, susceptible under several experimental conditions, inoculation with different Fusarium species resulted in significantly higher DI and DS, compared with AS, which showed Fusarium resistance. Furthermore, the values of DI and DS were significantly correlated with the previous findings generated under several experimental conditions. The use of this simple and reliable method in barley breeding programs can speed up the process of identification of sources of resistance to multiple FHB isolates. To our best knowledge, this is the first in-depth report investigating the usefulness of DHA for distinguishing susceptibility of barley plants and aggressiveness of diverse Fusarium species from a breeder's point of view.

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“…Barley leaf inoculation experiment was performed as described by Bedawy et al (2018) with minor modifications. Fully developed second leaves from 12-day-old plants were briefly washed with 75% (v/v) ethanol for 5 s, and 3 times with sterile distilled water.…”

Section: Methodsmentioning

confidence: 99%

Knockout of MITOGEN-ACTIVATED PROTEIN KINASE 3 causes barley root resistance against Fusarium graminearum

et al. 2022

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The roles of mitogen-activated protein kinases (MAPKs) in plant-fungal pathogenic interactions are poorly understood in crops. Here, microscopic, phenotypic, proteomic and biochemical analyses revealed that roots of independent transcription activator-like effector nuclease (TALEN)-based knockout lines of barley (Hordeum vulgare L.) MITOGEN-ACTIVATED PROTEIN KINASE 3 (HvMPK3 KO) were resistant against Fusarium graminearum infection. When co-cultured with roots of the HvMPK3 KO lines, F. graminearum hyphae were excluded to the extracellular space, the growth pattern of extracellular hyphae was considerably deregulated, mycelia development was less efficient, and number of appressoria-like structures and their penetration potential were substantially reduced. Intracellular penetration of hyphae was preceded by the massive production of reactive oxygen species (ROS) in attacked cells of the wild type, but ROS production was mitigated in the HvMPK3 KO lines. Suppression of ROS production in these lines coincided with elevated abundance of catalase and ascorbate peroxidase. Moreover, differential proteomic analysis revealed downregulation of several defense-related proteins in wild type, and the upregulation of pathogenesis-related protein 1 (PR-1) and cysteine proteases in HvMPK3 KO lines. Proteins involved in suberin formation, such as peroxidases, lipid transfer proteins, and the GDSL esterase/lipase were differentially regulated in HvMPK3 KO lines after F. graminearum inoculation. Consistent with proteomic analysis, microscopic observations showed enhanced suberin accumulation in roots of HvMPK3 KO lines, most likely contributing to the arrested infection by F. graminearum. These results suggest that TALEN-based knockout of HvMPK3 leads to barley root resistance against Fusarium root rot.

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Mining the global diversity of barley for Fusarium resistance using leaf and spike inoculations

Cited by 8 publications

References 32 publications

Building on a foundation: advances in epidemiology, resistance breeding, and forecasting research for reducing the impact of fusarium head blight in wheat and barley

Building on a foundation: advances in epidemiology, resistance breeding, and forecasting research for reducing the impact of fusarium head blight in wheat and barley

A simple and reliable assay to quantify host resistance and aggressiveness of the pathogen in the Fusarium head blight-barley pathosystem

Knockout of MITOGEN-ACTIVATED PROTEIN KINASE 3 causes barley root resistance against Fusarium graminearum

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