Mining the prospective of Candida tropicalis YES3 in Napier biomass saccharification

Current advances in Candida tropicalis: Yeast overview and biotechnological applications

“…After optimizations of nitrogen and minerals content in the media, as well as some operational parameters (pH 6.5, 30°C, and 72 h), this strain reached 34.20 U/mL cellulase activity using carboxymethyl cellulose (CMC) as substrate and 25.49 U/mL using Napier grass biomass waste powdered. After 5 days of saccharification, 72.51% of cellulose content in Napier biomass was converted to glucose 91 . C. tropicalis W2 isolated from sites with a high level of plant cells decomposition was also a cellulase producer able to grow in several substrates such as CMC, filter paper, rice straw, and wheat bagasse 92 .…”

“…After 5 days of saccharification, 72.51% of cellulose content in Napier biomass was converted to glucose. 91 C. tropicalis W2 isolated from sites with a high level of plant cells decomposition was also a cellulase producer able to grow in several substrates such as CMC, filter paper, rice straw, and wheat bagasse. 92 This strain produced cellulase with an activity of 75 U/mg after 120 h fermentation of saw dust hydrolysate 1% supplemented with mineral salts at pH 7.0 and 37 • C. The yeast produced both intra and extracellular cellulases, with the extracellular form being predominant.…”

Probing cellulolytic yeast from forest ecosystem for the saccharification of Napier fodder biomass

“…Cellulase activity in microbes can be improved by nutritional and process parameters during fermentation (Rasul et al, 2015). Response surface methodology is an efficient technique utilized for optimization of fermentation processes (Valliammai et al, 2020). Mathematical models are proven tools in the optimisation of process parameters during fermentation (Imamoglu and Sukan, 2013).…”

“…Y = +23.87+ +1.26 A + 0.8132 B + 0.0277 C -0.1295 D -0.0195E -0.0135F +0.3186 AB -0.0902AC +0.0392AD - water. The decrease in enzymatic activity with increasing incubation time might be due to depletion of nutrients and production of other inhibitors during the fermentation medium (Ikram-ul-Haq et al, 2005 (Valliammai et al, 2020). RSM provided the individual and interactive effects of components in the medium during fermentation.…”

“…Yeasts like Pichiastipitis was utilized in the saccharification study of sugarcane bagasse (Lau et al, 2010). The saccharification potential of Candida tropicalis isolate YES3 in Napier grass rd biomass was maximum on 3 day (31.93%) (Valliammai et al, 2020).…”