MiniPCR as a portable equipment for the molecular diagnosis of american cutaneous leishmaniasis

Castellanos-González, Alejandro; Cossio, Alexandra; Jojoa, Jimena; Moen, Scott T.; Travi, Bruno L.

doi:10.1016/j.actatropica.2023.106926

Acta Tropica

2023

DOI: 10.1016/j.actatropica.2023.106926

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MiniPCR as a portable equipment for the molecular diagnosis of american cutaneous leishmaniasis

Alejandro Castellanos-González

Alexandra Cossio

Jimena Jojoa

et al.

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2024

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An Ultra-Compact and Low-Cost LAMP-Based Virus Detection Device

Guo,

Ling,

Tang

et al. 2024

Sensors

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Timely and accurate detection of viruses is crucial for infection diagnosis and treatment. However, it remains a challenge to develop a portable device that meets the requirement of being portable, powerless, user-friendly, reusable, and low-cost. This work reports a compact ∅30 × 48 mm portable powerless isothermal amplification detection device (material cost ∼$1 USD) relying on LAMP (Loop-Mediated Isothermal Amplification). We have proposed chromatographic-strip-based microporous permeation technology which can precisely control the water flow rate to regulate the exothermic reaction. This powerless heating combined with phase-change materials can maintain a constant temperature between 50 and 70 ∘C for a duration of up to 49.8 min. Compared with the conventional methods, it avoids the use of an additional insulation layer for heat preservation, greatly reducing the size and cost. We have also deployed a color card and a corresponding algorithm to facilitate color recognition, data analysis, and storage using a mobile phone. The experimental results demonstrate that our device exhibits the same limit of detection (LOD) as the ProFlex PCR for SARS-CoV-2 pseudovirus samples, with that for both being 103 copies/μL, verifying its effectiveness and reliability. This work offers a timely, low-cost, and easy way for respiratory infectious disease detection, which could provide support in curbing virus transmission and protecting the health of humans and animals, especially in remote mountainous areas without access to electricity or trained professionals.

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An Ultra-Compact and Low-Cost LAMP-Based Virus Detection Device

Guo,

Ling,

Tang

et al. 2024

Sensors

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show abstract

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MiniPCR as a portable equipment for the molecular diagnosis of american cutaneous leishmaniasis

Cited by 1 publication

References 30 publications

An Ultra-Compact and Low-Cost LAMP-Based Virus Detection Device

An Ultra-Compact and Low-Cost LAMP-Based Virus Detection Device

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