Minisatellites in genes encoding cell wall proteins: a new way towards wine strain characterisation

Marinangeli, Paola; Angelozzi, Daniele; Ciani, Maurizio; Clementi, Francesca; Mannazzu, Ilaria

doi:10.1016/s1567-1356(03)00172-7

Cited by 43 publications

(24 citation statements)

References 35 publications

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“…Four polymorphic minisatellite loci (Marinangeli et al 2004) were also successfully amplified from the yeast strains and the sizes of the loci estimated from their mobility on agarose gels ( Table 2, right side). The results from these markers were similar to those from the microsatellites, but an additional difference was obtained between Lalvin QA23 and the other four strains.…”

Section: Dna Fingerprinting Of Commercial Yeast Strainsmentioning

confidence: 99%

“…DNA sequence of the internal transcribed spacer (ITS) region was used to identify the species of a subset of strains. Detailed genetic comparisons of the strains were performed using molecular markers, including six published (Field and Wills 1998;Perez et al 2001) and seven novel microsatellite loci, as well as four published minisatellite loci (Marinangeli et al 2004). The approach clearly identified a range of different genetic types, with the exception that five of the strains were unable to be resolved.…”

Section: Introductionmentioning

confidence: 99%

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A homozygous diploid subset of commercial wine yeast strains

Bradbury

Richards

Niederer

et al. 2005

Antonie Van Leeuwenhoek

119

118

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Genetic analysis was performed on 45 commercial yeasts which are used in winemaking because of their superior fermentation properties. Genome sizes were estimated by propidium iodide fluorescence and flow cytometry. Forty strains had genome sizes consistent with their being diploid, while five had a range of aneuploid genome sizes that ranged from 1.2 to 1.8 times larger. The diploid strains are all Saccharomyces cerevisiae, based on genetic analysis of microsatellite and minisatellite markers and on DNA sequence analysis of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA of four strains. Four of the five aneuploid strains appeared to be interspecific hybrids between Saccharomyces kudriavzevii and Saccharomyces cerevisiae, with the fifth a hybrid between two S. cerevisiae strains. An identification fingerprint was constructed for the commercial yeast strains using 17 molecular markers. These included six published trinucleotide microsatellites, seven new dinucleotide microsatellites, and four published minisatellite markers. The markers provided unambiguous identification of the majority of strains; however, several had identical or similar patterns, and likely represent the same strain or mutants derived from it. The combined use of all 17 polymorphic loci allowed us to identify a set of eleven commercial wine yeast strains that appear to be genetically homozygous. These strains are presumed to have undergone inbreeding to maintain their homozygosity, a process referred to previously as 'genome renewal'.

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Section: Dna Fingerprinting Of Commercial Yeast Strainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A homozygous diploid subset of commercial wine yeast strains

Bradbury

Richards

Niederer

et al. 2005

Antonie Van Leeuwenhoek

119

118

View full text Add to dashboard Cite

show abstract

“…A large number of molecular techniques are now available to identify the phenotypic variability between and within the populations of any organism using both protein and DNA markers. They may be multilocus enzyme electrophoresis, MLEE (Regua-Mangia et al, 2004), restriction fragment length polymorphism, RFLP (Patel et al, 2004), ribotyping (Andollina et al, 2004), allele-specific amplification (Jarry et al, 2004), minisatellite targeted PCRs (Marinangeli et al, 2004), simple sequence repeat PCRs, SSR-PCR (Hennequin et al, 2001), inter-simple sequence repeat PCRs, ISSR-PCR (Yamagishi et al, 2002), random amplification of polymorphic DNA, RAPD (Chung et al, 2002;Wang et al, 2004;Welsh and McClelland, 1990;Zhou and Jiao, 2004), and sequence characterized amplified regions, SCAR (Behura et al, 1999;Brisse et al, 2000;Fernandez et al, 2004;Kengne et al, 2001;Manguin et al, 2002;Paran and Michelmore, 1993).…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Tropical Tasar Silkworm (Antheraea mylitta) Ecoraces with RAPD and SCAR Markers

Saha

Kundu

2006

Biochem Genet

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The tropical tasar silkworm, Antheraea mylitta, has several ecoraces, 10 of which are commercially exploited for the production of tasar silk. These ecoraces are identified by morphological markers that are greatly influenced by photoperiod, humidity, altitude, and host plants. The DNA markers, random amplification of polymorphic DNA (RAPD), and sequence-characterized amplified region (SCAR) are identified to complement the existing morphological markers. Seven RAPD bands are selected that identify 8 of the 10 ecoraces. These identified RAPD fragments are sequenced and primers are designed for SCAR markers. Of the seven sets of primers, a single primer pair produced polymorphic SCAR bands that diagnose 5 of the 10 ecoraces. All 10 ecoraces are identified by the use of RAPD and SCAR markers together.

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“…While the microsatellite method has been widely used among non-Saccharomyces species, the minisatellite approach has not. In S. cerevisiae, nuclear genes contain minisatellites that are repeated in blocks as sites of gene recombination that result in gene length polymorphism, and these represent interesting molecular targets for characterisation by PCR fingerprinting (Gonzalez Techera et al, 2001;Marinangeli et al, 2004).…”

Section: Introductionmentioning

confidence: 99%